Detection method of cancer blank antigen based on uio-66(nh2)-silver sulfide composite material

A composite material and antigen detection technology, applied in the direction of material inspection products, analytical materials, biological material analysis, etc., can solve the problems of low reliability of detection results, high detection limit, narrow linear range, etc., and achieve good photoelectrochemical stability The effect of high stability, low detection limit and wide linear range

Active Publication Date: 2022-06-21
DONGGUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the photoelectrochemical detection of CEA has the following disadvantages. On the one hand, the photoelectric activity and stability of the sensor need to be improved, and the reliability of the detection results is not high; on the other hand, the detection limit is high and the linear range is low. relatively narrow

Method used

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  • Detection method of cancer blank antigen based on uio-66(nh2)-silver sulfide composite material
  • Detection method of cancer blank antigen based on uio-66(nh2)-silver sulfide composite material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Synthesis of UiO-66(NH2)-silver sulfide composite material

[0024] S1: Synthesize UiO-66 (NH2), specifically, a, using solvothermal reaction method, ultrasonically dissolving zirconium tetrachloride and 2-aminoterephthalic acid in DMF solution, transfer to the reaction kettle, at 120 ℃ Under the reaction for 24h; b. Centrifuge the light yellow product obtained after the reaction in the reaction kettle in step a, wash it three times with a washing solvent, vacuum dry at 80 ° C, and let stand to obtain UiO-66 (NH 2 ) solid powder.

[0025] S2: modify a layer of UiO-66 (NH2) on the FTO glass, specifically: a. Cut the FTO glass into sheets, and ultrasonically clean the FTO glass in anhydrous ethanol and deionized water for 3 minutes in turn; b. The cleaned flake FTO glass was blown dry with nitrogen, and 2.5 mg mL was taken. -1 UiO-66(NH 2 ) dropwise evenly on the surface of the FTO glass, and air-dry naturally at room temperature.

[0026] S3: Ag2S is grown...

Embodiment 2

[0028] Example 2: Determination of CEA with UiO-66(NH2)-silver sulfide composite

[0029] Take the UiO-66(NH2)-silver sulfide in Example 1, install the FTO glass loaded with UiO-66(NH2)-silver sulfide composite material on the electrode, place it in the PBS buffer solution of ascorbic acid, energize, carry out Electrochemical reaction to detect carcinogenic antigen levels in ascorbic acid.

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Abstract

The present invention relates to the field of biological detection, in particular to a cancer blank antigen detection method based on UiO-66(NH2)-silver sulfide composite material, comprising the following steps: S1: synthesizing UiO-66(NH2); S2: modifying an FTO glass Layer UiO-66(NH2); S3: grow Ag2S on the surface of UiO-66(NH2) compound to obtain UiO-66(NH2)-silver sulfide composite material; S4: load UiO-66(NH2)-silver sulfide composite The material FTO glass is installed on the electrode, placed in the PBS buffer solution of ascorbic acid, electrified, and electrochemical reaction is carried out to detect the level of cancer blank antigen in the ascorbic acid. The detection method of the invention has low detection cost, good detection result reliability, low detection limit and wide linear range.

Description

technical field [0001] The invention relates to the field of biological detection, in particular to a cancer blank antigen detection method based on UiO-66 (NH2)-silver sulfide composite material. Background technique [0002] CEA belongs to one of the embryonic antigens and is a normal component because it is produced by embryonic tissue during embryonic development. As the embryo grows, the content of late embryonic antigens in the embryo decreases, and it gradually disappears or only exists in very small amounts after birth. When the cells become cancerous, such antigens can be re-synthesized, expressed on the surface of tumor cells, or secreted into the blood. Their content is often positively correlated with the degree of cell malignancy. Therefore, CEA is often used in clinical diagnosis to detect tumors. Important indicators. At present, the immunological methods for the determination of CEA mainly include enzyme-linked immunosorbent assay, fluorescence immunoassay,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/327G01N27/36G01N27/30G01N27/38G01N33/574G01N33/68
CPCG01N27/3278G01N27/36G01N27/305G01N27/38G01N33/57473G01N33/6893G01N2333/4746
Inventor 张敏宾倩韵程发良谢永泽谢世磊柳鹏王寿山谢东
Owner DONGGUAN UNIV OF TECH
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