Detection method of cancer blank antigen based on uio-66(nh2)-silver sulfide composite material
A composite material and antigen detection technology, applied in the direction of material inspection products, analytical materials, biological material analysis, etc., can solve the problems of low reliability of detection results, high detection limit, narrow linear range, etc., and achieve good photoelectrochemical stability The effect of high stability, low detection limit and wide linear range
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Embodiment 1
[0023] Example 1: Synthesis of UiO-66(NH2)-silver sulfide composite material
[0024] S1: Synthesize UiO-66 (NH2), specifically, a, using solvothermal reaction method, ultrasonically dissolving zirconium tetrachloride and 2-aminoterephthalic acid in DMF solution, transfer to the reaction kettle, at 120 ℃ Under the reaction for 24h; b. Centrifuge the light yellow product obtained after the reaction in the reaction kettle in step a, wash it three times with a washing solvent, vacuum dry at 80 ° C, and let stand to obtain UiO-66 (NH 2 ) solid powder.
[0025] S2: modify a layer of UiO-66 (NH2) on the FTO glass, specifically: a. Cut the FTO glass into sheets, and ultrasonically clean the FTO glass in anhydrous ethanol and deionized water for 3 minutes in turn; b. The cleaned flake FTO glass was blown dry with nitrogen, and 2.5 mg mL was taken. -1 UiO-66(NH 2 ) dropwise evenly on the surface of the FTO glass, and air-dry naturally at room temperature.
[0026] S3: Ag2S is grown...
Embodiment 2
[0028] Example 2: Determination of CEA with UiO-66(NH2)-silver sulfide composite
[0029] Take the UiO-66(NH2)-silver sulfide in Example 1, install the FTO glass loaded with UiO-66(NH2)-silver sulfide composite material on the electrode, place it in the PBS buffer solution of ascorbic acid, energize, carry out Electrochemical reaction to detect carcinogenic antigen levels in ascorbic acid.
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