Method for preparing coprinus comatus cultivation seeds by using corn grains

A production method and technology of corn kernels, which are applied in mushroom cultivation, cultivation, plant cultivation, etc., can solve the problems of long cycle and achieve the effects of shortened production cycle, good growth consistency, and robust growth

Inactive Publication Date: 2020-02-18
辽宁省微生物科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In the cultivation process, the production of cultivated species is very important. At present, the production is to connect the original species to the surface of the culture material of the sterilized cultivated species. After the hyphae germinate, they grow from the surface downward. The cycle is generally 30 days, which is longer.

Method used

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  • Method for preparing coprinus comatus cultivation seeds by using corn grains
  • Method for preparing coprinus comatus cultivation seeds by using corn grains
  • Method for preparing coprinus comatus cultivation seeds by using corn grains

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Embodiment Construction

[0029] The present invention relates to a kind of preparation method of Coprinus comatus corn grain bacterial classification, it is to carry out according to the following steps:

[0030] 1. Strain selection

[0031] Select special white coprinus comatus (Coprinus comatus) Lccc 50647 as the provenance;

[0032] Second, the production of liquid bacteria

[0033] (1) Take 20g of corn flour and make a paste, boil the water, add 10g of bean cake powder, 10g of glucose, 2g of potassium dihydrogen phosphate, and 1.5g of magnesium sulfate, then slowly add the corn flour that has been made into a paste, and finally use a mass of Adjust the pH to 7.0 with 5% hydrochloric acid or 5% sodium hydroxide solution, filter and dilute to 1L to obtain a culture medium for later use;

[0034] (2) Sterilization and cooling: Divide the culture solution into 500mL triangular flasks, fill the bottles with 150mL culture solution, then plug the mouth of the bottle tightly with a cotton plug, and then...

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Abstract

The invention relates to a method for preparing coprinus comatus cultivation seeds by using corn grains, which includes selecting white coprinus comatus strains as provenances, wherein the compositionof a culture medium comprises corn flour, glucose, bean cake powder, potassium dihydrogen phosphate, magnesium sulfate and water; sterilizing the culture medium according to a conventional method, inoculating white coprinus comatus, and culturing on a shaking table to obtain white coprinus comatus liquid strains;mixing the corn grains and a nutrient solution to obtain a corn grain strain culturemedium, wherein the nutrient solution comprises potassium dihydrogen phosphate, magnesium sulfate, vitamin B1 and water; and inoculating the white coprinus comatus liquid strains to the sterilized corn grain strain culture medium for mycelium culture. The method has the advantages that corn grains are used as raw materials, a reasonable composition is adopted, liquid strains are inoculated, and the liquid strains are directly inoculated to the sterilized corn grain culture medium, so that the fluidity of the liquid strains and the rolling property of the corn grain culture medium are fully utilized, the hypha grows robustly, the growth consistency is good, the strain vitality is strong, the strain age is short, and the production cycle of the coprinus comatus cultivation seeds is shortened.

Description

technical field [0001] The invention relates to a method for preparing Coprinus comatus cultivars with corn kernels, which can realize a production cycle of 20 days for the Coprinus comatus cultivars. Background technique [0002] Coprinus comatus is rich in nutrition, delicious in taste, and has the characteristic of covering soil to produce mushrooms. It is a typical soil-born fungus. Coprinus comatus has a short growth cycle, high biotransformation rate, easy cultivation and considerable economic benefits. [0003] In the cultivation process, the production of cultivars is very important. At present, the production is to connect the original species to the surface of the culture material of the sterilized cultivars. After the mycelium germinates, it grows downward from the surface. The cycle is generally 30 days, which is longer. Contents of the invention [0004] In order to solve the problems existing in the prior art, the present invention provides a production meth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/00A01G18/20
CPCA01G18/00A01G18/20
Inventor 孟庆国池景良陈飞朱巍巍邓春海王艳华徐冲陈德伟
Owner 辽宁省微生物科学研究院
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