Application of spermidine in preparation of medicines for treating colitis

A technology of colitis and nitrile amines, which is applied in the field of application of nitrile amines in the preparation of drugs for treating colitis, and can solve problems such as incomplete understanding of the pathogenesis

Inactive Publication Date: 2020-02-18
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the pathogenesis of inflammatory bowel disease has been extensively studied, its exact pathogenesis is not fully understood

Method used

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  • Application of spermidine in preparation of medicines for treating colitis
  • Application of spermidine in preparation of medicines for treating colitis
  • Application of spermidine in preparation of medicines for treating colitis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 animal treatment

[0025] (1) 7-week-old male C57BL / 6 mice were reared under standard experimental conditions: 25±2°C, 50±5% humidity, 12h / 12h light / dark cycle. After one week of adaptation, it was used for experiments.

[0026] (2) Select male adult (8 weeks) mice with similar body weight in step (1) and divide them into four groups at random, marked as blank control group, experimental model group, low-dose spermidine group and high-dose spermidine group ;

[0027] (3) High-pressure sterilized distilled water was prepared with a mass concentration of 5% DSS solution, and the experimental model group, the low-dose spermidine group and the high-dose spermidine group were given DSS solution to drink, and the mice in the blank control group and the experimental model group normally drank high pressure Sterilized distilled water was used to ensure sufficient daily drinking water for mice during the experiment;

[0028] (4) Prepare an aqueous solution of spe...

Embodiment 2

[0031] Embodiment 2 fecal occult blood test (o-toluidine method)

[0032] Pick up a small amount of feces with a bamboo stick and spread it on a white porcelain plate; add 2-3 drops of 10g / L o-toluidine glacial acetic acid solution on the feces; add 2-3 drops of 3% hydrogen peroxide; observe the results immediately , showing blue within 2 minutes is positive;

[0033] critical result:

[0034] Negative: No color develops within 2 minutes after adding the reagent;

[0035] Positive (+): After adding the reagent for 10s, it changes from light blue to blue gradually;

[0036] Positive (++): After adding the reagent, it initially appears light blue-brown, and gradually becomes obvious blue-brown;

[0037] Positive (+++): After adding the reagent, it turns blue-brown immediately;

[0038] Positive (++++): Immediately after adding the reagent, it turns blue and dark brown.

Embodiment 3

[0039] Embodiment 3 mouse disease activity index score

[0040] The degree of colitis in mice was determined based on the disease activity index (DAI) score. The disease activity index score was composed of three parts: body weight change, blood in the stool score, and feces character score. The specific calculation method of DAI was: DAI = (body weight change score mean + blood in stool mean score + feces score mean) / 3.

[0041] 1. Scoring of body weight change: In Example 1, the body weight change standard of each group of mice was based on the body weight on day 0, and was scored according to the daily body weight change rate. 0 points, no weight loss; 1 point, 20% weight loss.

[0042] 2. Blood in stool score (measured by the method in Example 2)

[0043] 0 points, negative fecal occult blood test; 1 point, positive fecal occult blood test (including the 4 positive cases in Example 2); 2 points, gross bloody stool; 3 points, rectal bleeding.

[0044] 3. Fecal property s...

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Abstract

The present invention discloses an application of spermidine in preparation of medicines for treating colitis. A mouse colitis model is constructed in a model mouse, a phenotypic effect of the spermidine on mice with the colitis is studied, and the spermidine alleviates mouse inflammation-related phenotypes of weight loss, colonic shortening, hematochezia, etc.; a cell inflammatory state stimulated by TNF-alpha is constructed in human colon cancer cells in vitro, and after a spermidine treatment, inflammatory signaling pathway protein expression and downstream gene expression are obviously improved. To sum up, the spermidine improves the phenotype of the inflammation in vivo and also further proves an effect on inhibiting the inflammation through in-vitro cell experiments at a molecular level.

Description

[0001] (1) Technical field [0002] The invention relates to the application of spermidine, a polyamine substance, mainly in the preparation and development of drugs related to inflammatory bowel disease such as colitis and functional food. [0003] (2) Background technology [0004] Inflammatory bowel disease is a common chronic intestinal inflammatory disease arising in the small and large intestines, including Crohn's disease and ulcerative colitis. Crohn's disease can affect any part of the intestine, whereas ulcerative colitis primarily involves lesions in the large intestine. The main symptoms of inflammatory bowel disease include fever, diarrhea, abdominal pain, blood in the stool, and loss of appetite. Although a great deal of research has been done on the pathogenesis of inflammatory bowel disease, its exact pathogenesis is not fully understood. Genetic, environmental, nutritional and infectious factors are all closely related to the occurrence of inflammatory bowel ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/132A61P1/00A23L33/00
CPCA23V2002/00A61K31/132A23L33/00A61P1/00A23V2200/32
Inventor 倪银华马灵燕傅正伟
Owner ZHEJIANG UNIV OF TECH
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