Preparation and purpose of MMP response type nanometer adriamycin-indocyanine green carrying hydrogel

A technology of indocyanine green water and indocyanine green, which is used in medical preparations containing active ingredients, drug delivery, photodynamic therapy, etc. Concentration of action, convenience of operation, and effect of reducing cardiotoxicity

Inactive Publication Date: 2020-02-18
温宁
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the past 20 years, the overall survival rate of this type of disease has not improved significantly, and traditional treatment methods have their own limitations

Method used

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  • Preparation and purpose of MMP response type nanometer adriamycin-indocyanine green carrying hydrogel
  • Preparation and purpose of MMP response type nanometer adriamycin-indocyanine green carrying hydrogel
  • Preparation and purpose of MMP response type nanometer adriamycin-indocyanine green carrying hydrogel

Examples

Experimental program
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Embodiment 1

[0026] (1) Dissolve 20 mg of doxorubicin hydrochloride in 4 mL of DMSO, add dropwise 16 uL of triethylamine to remove the hydrochloric acid, stir for 5 min, add 80 mg of Poloxamer188 and stir to fully dissolve it, and obtain doxorubicin dimethylmethylene dehydrochloride Sulfone solution. The raw material solution was poured into the anti-solvent PBS, and fully mixed to obtain a doxorubicin nano-drug suspension, which was dialyzed with deionized water for 4 hours (repeat 3 times), and freeze-dried to obtain adriamycin nano-drug powder.

[0027] (2) Dissolve 20 mg of indocyanine green in 4 mL of DMSO, add 80 mg of Poloxamer 188 and stir to fully dissolve it to prepare a doxorubicin dimethyl sulfoxide dehydrochloride solution. Pour the raw material solution into the anti-solvent PBS, mix well to obtain a doxorubicin nano drug suspension, dialyze with deionized water for 4 hours (repeat 3 times), and freeze-dry to obtain indocyanine green drug powder.

[0028] (3) Accurately weig...

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PUM

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Abstract

The invention relates to MMP response type nanometer adriamycin-indocyanine green carrying hydrogel. A preparation method of the MMP response type nanometer adriamycin-indocyanine green carrying hydrogel comprises the following steps of: (1) preparing nanometer adriamycin granules and nanometer indocyanine green granules by an anti-solvent method, and performing freeze-drying and storage; and (2)accurately weighing a given amount of nanometer adriamycin freeze-dried powder and a given amount of nanometer indocyanine green freeze-dried powder, adding a TEA buffer solution, performing uniform mixing, accurately weighing a given quantity of acrylic ester-hyaluronic acid derivatives (HA-AC), adding the TEA buffer solution, performing uniform mixing, mixing two solutions in a certain proportion, adding an HS-MMP-SH cross-linking agent, performing placing in a thermotank of 37 DEG C, and performing cross-linking to obtain gel. According to the preparation method disclosed by the invention,MMP response type hydrogel is used as a carrier to wrap the nanometer adriamycin and nanometer indocyanine green, the MMP response type nanometer adriamycin-indocyanine green carrying hydrogel is injected into tumor positions in situ, and tumor cells can secrete a large amount of MMP, so that the hydrogel can be responded in the tumor position, degradation is accelerated, and controlled release and slow release of medicines can be realized; and besides, phototherapy and chemotherapy are united, so that the tumor restraining efficiency can be strengthened, and side effects of heart toxicity, skin phototoxicity and the like can be reduced.

Description

technical field [0001] The invention relates to the field of pharmacy, in particular to a drug-loaded hydrogel sensitive to MMP and its preparation method and application. Background technique [0002] Head and neck squamous cell carcinoma is the sixth most common cancer in the world. Over the past 20 years, both new cases and deaths from this disease have shown an increasing trend year by year. In the United States, there are about 50,000 new cases and about 10,000 deaths each year. The incidence of head and neck cancer in my country has leapt to the sixth place in the incidence of malignant tumors, and it has become a type of cancer that seriously threatens the health of Chinese residents. The current treatment methods for head and neck squamous cell carcinoma are still based on traditional surgery, chemotherapy and radiotherapy. However, in the past 20 years, the overall survival rate of this type of disease has not improved significantly, and the traditional treatment...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61P35/00A61K31/704
CPCA61K9/0019A61K31/704A61K41/0057A61P35/00A61K2300/00
Inventor 王欢欢付之光张建军李云霞王宇温宁
Owner 温宁
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