Feed compound additive for abalones as well as preparation and use method of additive
A technology for additives and feeds, applied in the field of compound feed additives for aquaculture, can solve problems such as unsatisfactory effects, and achieve the effects of solving old abalone seedlings, promoting abalone growth and improving immunity
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Embodiment 1
[0017] Thaw the collected fresh sea squirts or frozen sea squirts, drain the sea water, rinse with fresh water, drain the water, and collect the individuals. Crush the collected Ciona with a pulverizer, filter it with gauze, add appropriate amount of pure water and homogenize it with a digital display high-speed dispersing homogenizer, adjust the pH to 6.5, add 0.1% compound protease for enzymatic hydrolysis, and inactivate the enzyme at 100°C, 4500r / min After centrifuging for 30 min, the supernatant was taken to obtain Ciona polypeptide extract, wherein the determined polypeptide content was 1.6%.
[0018] Add probiotics and multivitamins to the sea squirt polypeptide extract, the probiotics are Bacillus, and the ratio of the multivitamins is vitamin E:vitamin A:vitamin B2:vitamin B=2:2:1:1; the weight percentage of the three 80% of ascidian polypeptide extract, 16% of probiotics, and 4% of multivitamins are used to make abalone feed compound additives.
[0019] How to use: ...
Embodiment 2
[0021] The preparation method of the sea squirt polypeptide extract in this example is consistent with the steps in Example 1, the amount of compound protease added during enzymatic hydrolysis is 0.2%, and the polypeptide content in the obtained extract is 1.8%.
[0022] Add probiotics and multivitamins to the polypeptide extract. The probiotics are used in combination with Bacillus aquaticus and plant lactic acid bacteria. The ratio of the two is 2:1. The proportion of each component in the multivitamin is vitamin E: vitamin A: vitamin B2: Vitamin B=2:2:1:1; the percentage by weight of the three is 85% of ascidian polypeptide extract, 12% of probiotics, and 3% of multivitamin, and it is made into abalone feed compound additive.
[0023] How to use: Mix the above-mentioned compound additive with abalone seedling feed, the mixing ratio is: abalone seedling feed: compound additive = 10:1, after mixing evenly, the feed is moist and not agglomerated, loose, ready for use; feed ever...
Embodiment 3
[0031] The preparation method of the sea squirt polypeptide extract in this example is consistent with the steps in Example 1, the amount of compound protease added during enzymolysis is 0.3%, and the polypeptide content in the obtained extract is 1.96%.
[0032] Add probiotics and multivitamins to the polypeptide extract. The probiotics use plant lactic acid bacteria. The proportion of each component in the multivitamins is vitamin E: vitamin A: vitamin B2: vitamin B=2:2:1:1; the ratio of the three The percentage by weight is 82.5% of polypeptide extract, 14% of probiotics, and 3.5% of multivitamins, and is made into a compound additive for abalone feed.
[0033] How to use: Mix the above-mentioned compound additive with abalone seedling feed, the mixing ratio is: abalone seedling feed: compound additive = 8:1, after mixing evenly, the feed will be moist and non-caking, loose, and ready for use; feed every evening Feed once, and the feeding amount accounts for 0.5% of the wei...
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