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Preparation method and application of autonomous nano generator pharmaceutical composition based on probiotic spores

A technology of probiotics and generators, applied in the field of preparation of autonomous nano-generator pharmaceutical compositions, can solve problems such as no public reports, achieve huge economic and social benefits, simple preparation methods, and stable gastric acid environment

Inactive Publication Date: 2020-02-21
ZHENGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The construction of this autonomous nanogenerator opens a new chapter in oral drug delivery and improves the bioavailability of oral drug delivery, but there has been no public report so far

Method used

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  • Preparation method and application of autonomous nano generator pharmaceutical composition based on probiotic spores
  • Preparation method and application of autonomous nano generator pharmaceutical composition based on probiotic spores
  • Preparation method and application of autonomous nano generator pharmaceutical composition based on probiotic spores

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] In the specific implementation of the present invention, a preparation method of an autonomous nano generator pharmaceutical composition based on probiotic spores comprises the following steps:

[0027] (1) Cultivate Bacillus coagulans: place Bacillus coagulans in a constant temperature shaking incubator at 37°C with a shaking speed of 150r / min, use nutrient gravy agar to culture Bacillus coagulans, and use Gram staining to observe when the bacteria grow to the logarithmic phase , collecting the obtained bacterial solution for subsequent use;

[0028] (2) Extract spores: first configure 0.005% MnSO 4 ·H 2 Nutrient gravy agar spore-forming medium of O, and then add the 5% volume (V:V) of the bacterial solution obtained in the step (1) culture, and place it in a constant temperature shaking incubator at 37°C, and continue to cultivate for 48h at a shaking speed of 150r / min; Gram staining was used to observe that one end of the bacteria began to expand, and the culture w...

Embodiment 2

[0032] In the specific implementation of the present invention, a preparation method of an autonomous nano generator pharmaceutical composition based on probiotic spores comprises the following steps:

[0033] (1) Cultivate Bacillus coagulans: place Bacillus coagulans in a constant temperature shaking incubator at 37°C with a shaking speed of 160r / min, use nutrient gravy agar to culture Bacillus coagulans, and use Gram staining to observe when the bacteria grow to the logarithmic phase , collecting the obtained bacterial solution for subsequent use;

[0034] (2) Extract spores: first configure 0.006% MnSO 4 ·H 2 Nutrient gravy agar spore-forming medium of O, and then add the 3% volume (V:V) of the bacterial solution obtained in the step (1) culture, and place it in a constant temperature shaking incubator at 37°C, and continue to cultivate for 48h at a shaking speed of 160r / min; Gram staining was used to observe that one end of the bacteria began to expand, and the culture w...

Embodiment 3

[0038] In the specific implementation of the present invention, a preparation method of an autonomous nano generator pharmaceutical composition based on probiotic spores comprises the following steps:

[0039] (1) Cultivate Bacillus coagulans: place Bacillus coagulans in a constant temperature shaking incubator at 37°C with a shaking speed of 180r / min, use nutrient gravy agar to culture Bacillus coagulans, and use Gram staining to observe when the bacteria grow to the logarithmic phase , collecting the obtained bacterial solution for subsequent use;

[0040] (2) Extract spores: first configure 0.008% MnSO 4 ·H 2 O’s nutrient broth agar spore-forming medium, and then add the bacterial solution obtained in step (1) with a volume of 4% (V:V), and place it in a 37°C constant temperature shaking incubator at a shaking speed of 180r / min to continue culturing for 48h; Gram staining was used to observe that one end of the bacteria began to expand, and the culture was continued for 7...

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Abstract

The invention relates to a preparation method and application of an autonomous nano generator pharmaceutical composition based on probiotic spores, can effectively solve the problems that a drug is easy to degrade in the stomach after being orally taken and the dilemma that the drug is easy to take and difficult to transport in oral administration at present, and provides construction of an oral administration system. The method comprises the following steps: firstly culturing Bacillus coagulans to generate spores, then modifying deoxycholic acid which can be combined with a bile acid receptoron the spore surfaces through amidation reaction, and then physically loading anti-tumor drugs adriamycin and sorafenib to obtain the autonomous nano generator pharmaceutical composition based on theprobiotic spores. The composition has the advantages of simple preparation method, low production cost, stable gastric acid environment and the like, retains the regeneration capacity of the drug-loaded spores, integrates a biological carrier and chemotherapy, further efficiently delivers the drugs to systemic circulation, and provides technical support for tumor treatment of oral administration.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, and relates to a preparation method and application of an autonomous nano generator pharmaceutical composition based on probiotic spores. Background technique [0002] Bacillus coagulans ( Bacillus Coagulans, BC) is a "generally recognized as safe" lactic acid bacteria approved by the US Food and Drug Administration, which can colonize the intestinal microenvironment and produce a large amount of lactic acid, amino acids, vitamins, etc., to treat intestinal inflammation and regulate intestinal balance . It is worth noting that the BC's dormant spore (Spore) is wrapped in a thick hydrophobic protein shell, which is one of the most stable static structures in biology and can resist harsh acidic environments, toxic chemicals and extreme temperatures, etc. . In addition, the physiological activity of spores will be triggered by some nutrients in the intestinal microenvironment, so after...

Claims

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Application Information

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IPC IPC(8): A61K47/69A61K31/704A61K31/44A61K47/54A61P35/00B82Y5/00B82Y30/00B82Y40/00C12N1/20C12R1/07
CPCA61K31/704A61K31/44A61K47/6901A61K47/554B82Y5/00B82Y30/00B82Y40/00A61P35/00C12N1/20
Inventor 王蕾宋庆龄张云张振中高慧王珂郑翠霞赵洪娟孙玲玲
Owner ZHENGZHOU UNIV
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