Quassin compound as well as preparation method and application thereof
A technology of compounds and extracts, applied in the field of bittern compounds and their preparations, can solve the problems of tree vigor decline, leaf shedding, flower and fruit drop, etc., and achieve good poisonous effect
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Embodiment 1
[0036] Example 1: Preparation and structural characterization of compound 1-23
[0037] 1) Take 46 kg of dry stems of bitter wood, soak them in 95v / v% ethanol for 24 hours, heat and reflux for extraction 4 times (the amount of solvent added during each extraction is 5 times the weight of the raw material, and extract 3 hours each time), combine the extracts, The extract was concentrated under reduced pressure to obtain the extract.
[0038] 2) Suspend the extract in water, extract with ethyl acetate, collect the ethyl acetate part, concentrate, and obtain 572.0 g of extract. The extraction flow chart is as figure 1 As shown, the obtained extract is further separated to obtain the flow chart of each compound as figure 2 shown.
[0039] 3) Apply the extract to silica gel column chromatography, and use dichloromethane-methanol system (V / V, 100:1~3:1) gradient elution, and use TLC thin layer chromatography combined with 210nm ultraviolet lamp irradiation detection during the e...
Embodiment 2
[0089] Embodiment 2: the preparation of compound 1-23
[0090] Repeat Example 1, the difference is that acetone is used as solvent in step 1).
[0091] Compound 1-23 obtained in this example was subjected to structural characterization (infrared, NMR, etc.), and it was determined to be the target compound 1-23 of the present invention.
Embodiment 3
[0092] Embodiment 3: the preparation of compound 1-23
[0093] Repeat Example 1, except that 70v / v% methanol is used as the solvent in step 1).
[0094] Compound 1-23 obtained in this example was subjected to structural characterization (infrared, NMR, etc.), and it was determined to be the target compound 1-23 of the present invention.
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