Plant senescence-related protein ghwrky91 and its coding gene and application

A protein and plant technology, applied in the field of plant aging-related protein GhWRKY91 and its coding gene and application, can solve the problems of short-season cotton premature aging

Active Publication Date: 2022-02-11
INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The contradiction between grain and cotton competing for land in our country is prominent, and the selection and promotion of short-season cotton varieties can alleviate the contradiction of grain and cotton competing for land, but the early maturity of short-season cotton is often accompanied by premature aging

Method used

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  • Plant senescence-related protein ghwrky91 and its coding gene and application
  • Plant senescence-related protein ghwrky91 and its coding gene and application
  • Plant senescence-related protein ghwrky91 and its coding gene and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0061] 1. Construction of recombinant expression vector

[0062] 1. Extract the mature leaves of the upland cotton Zhongmiansuo No. 74 plant at the full flowering stage, and extract the total RNA.

[0063] 2. Reverse transcribe the total RNA obtained in step 1 to obtain cDNA.

[0064] 3. Using the cDNA obtained in step 2 as a template, perform PCR amplification with a primer pair composed of F1 and R1, and recover the PCR amplification product.

[0065] F1: 5'-CTAG TCTAGA ATGGACAGCGGTGGTAG-3';

[0066] R1: 5'-TCC CCCGGG TCAGCAGATCCCAACCTG-3'.

[0067] 4. Digest the PCR amplified product obtained in step 3 with restriction endonucleases XbaI and SmaI, and recover the digested product.

[0068] 5. Digest the pBI121 plasmid with restriction endonucleases XbaI and SmaI to recover the vector backbone.

[0069] 6. Ligate the digested product obtained in step 4 with the vector backbone obtained in step 5 to obtain recombinant plasmid pBI121-GhWRKY91. According to the sequenc...

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Abstract

The invention discloses a plant aging-related protein GhWRKY91, its coding gene and application. The protein provided by the present invention is the protein shown in Sequence 1 in the sequence listing. Nucleic acid molecules encoding the protein also belong to the protection scope of the present invention. The invention also protects the application of the protein: regulating the aging process of plants; regulating the aging process of plant leaves; inhibiting the aging process of plants; inhibiting the aging process of plant leaves; regulating the aging process of plants in drought stress environments; regulating the aging process of plant leaves in drought stress environments Senescence process; regulation of drought resistance in plants; enhancement of drought resistance in plants. The present invention also protects a method for preparing a transgenic plant, which includes the following steps: introducing the gene into the starting plant to obtain a transgenic plant with delayed aging process. The invention has great theoretical value for the research on the mechanism of plant aging. It has great application value for breeding anti-premature plants, especially for cultivating anti-premature cotton.

Description

technical field [0001] The invention relates to a plant aging-related protein GhWRKY91 and its coding gene and application. Background technique [0002] Senescence is an integral part of plant development and is the final stage of development. During aging, leaf cells undergo highly coordinated changes in structure, metabolism, and gene expression. The earliest and most visible change in cellular structure is the breakdown of chloroplasts. In terms of metabolism, carbon metabolism (photosynthesis) is replaced by catabolism of chloroplasts and macromolecular substances such as proteins, membrane lipids, RNA, etc. At the molecular level, the changes described above are accompanied by changes in gene expression. [0003] Senescence is programmed cell death. Leaf senescence plays an important role in physiological activities such as recycling and reutilization of nutrient elements. Leaf assimilation function decline caused by senescence limits crop yield. [0004] Cotton ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/12A01H6/20A01H6/60
CPCC07K14/415C12N15/8273C12N15/8261
Inventor 喻树迅顾丽姣魏恒玲王寒涛王聪聪马亮苏政政
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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