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Mass spectrometry method for high-throughput detection of primary metabolites in corn pollen

A technology for corn pollen and metabolites, applied in the field of chemical detection, can solve problems such as no reports, and achieve the effect of high stability and good repeatability

Active Publication Date: 2020-03-13
AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on corn pollen is mostly focused on the determination of vigor, wall breaking, single or several chemical components analysis, health effects, etc., while the qualitative and quantitative analysis of primary metabolites in pollen (primary metabolome ) method research has not been reported yet

Method used

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  • Mass spectrometry method for high-throughput detection of primary metabolites in corn pollen
  • Mass spectrometry method for high-throughput detection of primary metabolites in corn pollen
  • Mass spectrometry method for high-throughput detection of primary metabolites in corn pollen

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Embodiment 1

[0023] The invention provides a technical scheme: a mass spectrometry method for high-throughput detection of primary metabolites in corn pollen, comprising the steps of:

[0024] S1. Pretreatment of pollen samples: Store the collected corn pollen in a liquid nitrogen tank at -196°C for 30 minutes to quench the metabolic reaction, and then transfer it to a -80°C refrigerator for storage. Put the pollen sample in a mortar, add liquid nitrogen to freeze and grind it into a powder to break the wall, put it into a 15mL centrifuge tube and store it in a -80°C refrigerator for analysis;

[0025] S2. Extraction of pollen metabolites: Accurately weigh 50 mg of corn pollen powder sample into a 2 mL centrifuge tube, add 1 mL of pre-cooled extract A and 10 μL 13 C-labeled D-ribitol (1mg / mL) internal standard, mix thoroughly until the sample is completely resuspended, then place the sample on a shaker, shake at 4°C, 300rpm for 10 minutes, shake Set the non-contact ultrasonic instrument t...

Embodiment 2

[0030] The invention provides a technical scheme: a mass spectrometry method for high-throughput detection of primary metabolites in corn pollen, comprising the steps of:

[0031] S1. Pretreatment of pollen samples: Store the collected corn pollen in a liquid nitrogen tank at -196°C for 30 minutes to quench the metabolic reaction, and then transfer it to a -80°C refrigerator for storage. Put the pollen sample in a mortar, add liquid nitrogen to freeze and grind it into a powder to break the wall, put it into a 15mL centrifuge tube and store it in a -80°C refrigerator for analysis;

[0032] S2. Extraction of pollen metabolites: Accurately weigh 50 mg of corn pollen powder sample into a 2 mL centrifuge tube, add 1 mL of pre-cooled extract A and 10 μL 13 C-labeled D-ribitol (1mg / mL) internal standard, mix thoroughly until the sample is completely resuspended, then place the sample on a shaker, shake at 4°C, 300rpm for 10 minutes, shake Set the non-contact ultrasonic instrument t...

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Abstract

The invention discloses a mass spectrometry method for high-throughput detection of primary metabolites in corn pollen. A common corn pollen metabolome extracting solution is firstly quickly analyzedby using a gas chromatography-mass spectrometry technology to obtain a comprehensive metabolic profile spectrum. 67 primary metabolites contained in the pollen are systematically identified for the first time. Then, a target metabolome analysis method is established for 67 primary metabolites, and quantitative analysis can be conducted on the corn pollen primary metabolome at a time. The method comprises the steps of sample pretreatment, chromatography-mass spectrometry separation, metabolite result identification and metabolite quantitative analysis. The method has the advantages of good repeatability, high stability, rapidness and reliability, and is suitable for primary metabolome analysis of corn pollen and other crop pollen samples.

Description

technical field [0001] The invention relates to the technical field of chemical detection, in particular to a mass spectrometry method for high-throughput detection of primary metabolites in corn pollen. Background technique [0002] Metabolism is a life process shared by living things, and the metabolism of plants can be divided into two processes: primary metabolism and secondary metabolism. Primary metabolites are an important metabolite of organisms, which provide energy and intermediate products for the growth, development and reproduction of living organisms, also known as nutrients. Metabolomics is a science that qualitatively and quantitatively quantifies all endogenous metabolites of cells in a biological system under given conditions, and then detects the whole and its response to changes in internal and external factors. Mainly based on common techniques such as GC-MS, LC-MS, NMR. [0003] Pollen is not only the fine particle in the stamen of the plant, but also...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 晏石娟黄文洁陈庄孔谦张群洁
Owner AGRO BIOLOGICAL GENE RES CENT GUANGDONG ACADEMY OF AGRI SCI
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