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Microbial agent for crop take-all disease and preparation method thereof

A technology of microbial inoculum and total erosion, which is applied in the fields of botanical equipment and methods, biocides, plant growth regulators, etc., can solve the problems of livestock and human health threats, crop resistance, and low control effect, etc. To achieve the effect of short cycle, convenient preparation and use, and reasonable ratio

Inactive Publication Date: 2020-03-24
河南农贝得农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Aiming at the defects that the above-mentioned existing chemical control is easy to cause drug resistance in crops, endangers the environment, poses a threat to livestock and human health, and other prevention methods have low control effect and poor imitation, the present invention provides a method for crop take-all disease Microbial agent and preparation method thereof

Method used

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  • Microbial agent for crop take-all disease and preparation method thereof

Examples

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Effect test

Embodiment 1

[0028] A kind of microbial inoculum for crop take-all, in parts by weight, said microbial inoculum includes 15 parts of Trichoderma, 15 parts of Bacillus amyloliquefaciens, 15 parts of yeast, 2000 parts of fish protein; also includes water, In terms of parts by weight, water is 25,000 parts; the spore content of Trichoderma is not less than 100 billion / g; the spore content of P. amyloliquefaciens is not less than 100 billion / g; the spore content of yeast is not less than 5 billion / g.

[0029] A kind of preparation method of the microbial inoculant against crop take-all, comprises the following steps, add trichoderma, bacillus amyloliquefaciens, yeast, fish protein into fermenter, add the water of above-mentioned parts again, after stirring evenly, in 22 It is obtained by aerobic fermentation at ℃ for 60 hours.

[0030] Application method: seed dressing and / or spray.

Embodiment 2

[0032] A kind of microbial inoculum for crop take-all disease, in parts by weight, said microbial inoculum includes Trichoderma 17, Pseudomonas amylolyticus 18, 15-20 parts of saccharomyces, 2500 parts of fish protein; also includes water, In terms of parts by weight, 26500 parts of water; the spore content of Trichoderma is not less than 100 billion / g; the spore content of P. amyloliquefaciens is not less than 100 billion / g; the spore content of yeast is not less than 5 billion / g.

[0033] A kind of preparation method of the microbial inoculant against crop take-all, comprises the following steps, adding Trichoderma, Bacillus amyloliquefaciens, yeast, fish protein to fermenter, adding the above-mentioned parts of water again, after stirring evenly, in 25 It is obtained by aerobic fermentation at ℃ for 48 hours.

[0034] Application method: seed dressing and / or spray.

Embodiment 3

[0036] A kind of microbial inoculum for crop take-all, in parts by weight, said microbial inoculum includes 20 parts of Trichoderma, 20 parts of Bacillus amyloliquefaciens, 20 parts of yeast, 3000 parts of fish protein; also includes water, In parts by weight, 28,000 parts of water; the spore content of Trichoderma is not less than 100 billion / g; the spore content of Bacillus amyloliquefaciens is not less than 100 billion / g; the spore content of yeast is not less than 5 billion / g.

[0037] A kind of preparation method of the microbial inoculant against crop take-all, comprises the following steps, add trichoderma, bacillus amyloliquefaciens, yeast, fish protein into fermenter, add the water of above-mentioned parts again, after stirring evenly, in 27 It is obtained by aerobic fermentation at ℃ for 40 hours.

[0038] Application method: seed dressing and / or spray.

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Abstract

The invention provides a microbial agent for the crop take-all disease and a preparation method thereof, and belongs to the technical field of biological products. The microbial agent comprises the following components in parts by weight: 15 to 20 parts of trichoderma, 15 to 20 parts of bacillus amyloliquefaciens, 15 to 20 parts of saccharomycetes, 2000 to 3000 parts of fish protein and 25,000 to28,000 parts of water. The preparation method of the microbial agent comprises the following steps: adding trichoderma, bacillus amyloliquefaciens, saccharomycetes and fish protein into a fermentationtank, adding the water, stirring the components to a uniform state, and carrying out aerobic fermentation at 22-27 DEG C for 40-60 hours, thereby obtaining the microbial agent. The strain disclosed by the invention generates new metabolites, namely, piperidine acid, floxacin and allobarbital, in an aerobic fermentation process, and the new metabolites can interact with other metabolites to prevent and treat the wheat take-all disease.

Description

technical field [0001] The invention relates to the technical field of biological products, in particular to a microbial agent for crop take-all and a preparation method thereof. Background technique [0002] Take-all disease, also known as blight or blackfoot, is a root disease that only infects 1-2 nodes at the base of wheat roots and stems. The diseased plants at the seedling stage are short, with many yellow leaves in the lower part, and the seed roots and underground stems turn gray-black, and in severe cases, the wheat seedlings die in pieces. In addition, there are corn take-all diseases and so on. [0003] The pathogens are Gaeumannomyces graminis var.graminis (Sacc.) Walker and Gaeumannomyces graminis (Sacc.) Arx et Olivervar.tritici (Sacc.) Walker), which belong to Ascomycetes Subphylum fungi do not produce asexual spores under natural conditions. [0004] Wheat take-all is a soil resident fungus. The fungus mainly survives the winter and summer on the diseased ...

Claims

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Application Information

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IPC IPC(8): A01N63/38A01N63/22A01N63/32A01P3/00
CPCA01N63/00
Inventor 凌新萍孙相龙周平运胡鉴顾腾飞
Owner 河南农贝得农业科技有限公司
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