Influenza virus nucleoprotein antigen protection liquid, and reagent and kit containing same

A technology of influenza virus and nucleoprotein, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of poor stability and achieve good thermal acceleration stability, high reactivity and signal-to-noise ratio

Active Publication Date: 2020-03-24
ZHUHAI LIVZON DIAGNOSTICS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology provides improved solutions for preventing viruses from infecting humans during flu seasonal outbreaks. It involves adding certain substances like tris(hydroxypropyl)ammonium chloride (Tris), which helps keep harmful strains harmless while still allowing them to bind strongly to their proteins inside cells. Additionally, this method uses specific buffers made up mostly of glycerol monomethyl ester instead of water to improve reaction time between different components within the vaccines. Overall, these technical improvements help make the immunization process more effective against emerging pandemics such as COVID-19.

Problems solved by technology

This patented technical solution involves developing new drugs able to improve the effectiveness of vaccination strategies aim at reducing both morbidities associated with current strains of human cold fever like measles and mumps while maintaining their ability to prevent future outbreaks from existing diseases such as pneumonia.

Method used

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  • Influenza virus nucleoprotein antigen protection liquid, and reagent and kit containing same
  • Influenza virus nucleoprotein antigen protection liquid, and reagent and kit containing same
  • Influenza virus nucleoprotein antigen protection liquid, and reagent and kit containing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Dissolve BSA, NaCl, Tween20 and ProClin300 in the following buffers: phosphate (PH7.4), citric acid buffer (PH6.0), 2-morpholineethanesulfonic acid (PH7.4), borate (PH8.0), carbonate (PH9.0), acetate (PH5.0), 4-hydroxyethylpiperazineethanesulfonic acid (PH7.0), Tris buffer (PH7.4), to The concentration of each component is BSA0.5w / v%, NaCl 150mM, Tween20 0.1w / v% and ProClin300 0.05w / v%; the concentration of buffer substances in each buffer is 20mM, and the pH value in the brackets is the buffer pH. Influenza virus nucleoprotein antigen protection solutions prepared in different buffers were diluted to prepare different concentrations of influenza NP antigens as samples, which were detected with fluorescent influenza antigen detection reagents (double antibody sandwich method) and the fluorescent signal values ​​were recorded. The reactivity was tested with influenza antigen detection reagent.

[0058] The results are shown in Table 1.

[0059] Table 1

[0060]

...

Embodiment 2

[0064] Dissolve BSA, NaCl, Tween20 and ProClin300 in Tris buffers of various concentrations and pHs as shown in Table 2, respectively, until the concentration of each component is BSA 0.5w / v%, NaCl150mM, Tween20 0.1w / v% and ProClin300 0.05w / v%, the prepared influenza virus nucleoprotein antigen protection solution was diluted to prepare different concentrations of influenza NP antigen as samples, and it was detected by fluorescent influenza antigen detection reagent (double antibody sandwich method) and analyzed. Record the fluorescent signal value. The results are shown in Table 2.

[0065] Table 2

[0066]

[0067]

[0068] It can be seen from the above that when the pH is 8-8.5, the fluorescence signal value of the influenza virus nucleoprotein antigen protection solution is higher, and the fluorescence signal value of Tris is higher when the concentration of Tris in the influenza virus nucleoprotein antigen protection solution is 20-50mM , wherein 20mM Tris buffer...

Embodiment 3

[0070] Prepare influenza virus nucleoprotein antigen protection solution, comprise 20mM Tris (pH8.0), 150mM NaCl, 0.1w / v%Tween20 and 0.05w / v%ProClin300 and the protein protection agent shown in table 3, protein protection agent comprises bovine Serum albumin (BSA), casein, fetal bovine serum, horse serum, concentration range from 0.5w / v% to 10w / v%. Different concentrations of influenza NP antigens were prepared by diluting each influenza virus nucleoprotein antigen protection solution as a sample to investigate its reactivity with influenza antigen detection reagents, and the results are shown in Table 3:

[0071] table 3

[0072]

[0073]

[0074] From the above, it can be seen that the effect of using BSA and fetal bovine serum as protein protective agents is better than other protein protective agents, of which 0.5w / v% bovine serum albumin, 1w / v% fetal bovine serum and 5w / v% fetal bovine serum As a protein protectant, serum has higher reactivity and signal-to-noise rat...

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Abstract

The invention provides an influenza virus nucleoprotein antigen protection liquid, and a reagent and a kit containing the same, and relates to the technical field of diagnostic reagents. The influenzavirus nucleoprotein antigen protection liquid comprises (a) 20 to 50 mM of Tris; (b) 0.5 to 2.5 mM of aminopyridine; (c) 0.5 to 10 w/v% of bovine serum albumin and/or 0.5 to 10 w/v% of fetal bovine serum; and, optionally, excipients. According to the influenza virus nucleoprotein antigen protection liquid, influenza virus nucleoprotein serving as an antigen has relatively high reactivity and signal-to-noise ratio; the influenza virus nucleoprotein antigen protective solution is used as a diluent of an influenza virus nucleoprotein antigen and a coating diluent for coating the influenza virusnucleoprotein antigen on a carrier, so that the influenza virus nucleoprotein antigen can keep better thermal acceleration stability and real-time stability.

Description

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Claims

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Application Information

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Owner ZHUHAI LIVZON DIAGNOSTICS
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