Fusion expression and purification method of cecropin antibacterial peptide

Abstract

The invention discloses a fusion expression and purification method of cecropin antibacterial peptide. The method comprises three steps: fusion expression of cecropin antibacterial peptide; treatmentof fusion expression products; and purification of fusion protein. According to the method, a self-induction fusion expression mode is adopted to replace the current commonly used IPTG induction, theexpression quantity of fusion protein is greatly improved, and a self-induction mode is adopted to reduce the cost due to too high price of IPTG; a mode of fusion protein denaturation direct sample loading purification and elution renaturation is adopted to purify the target protein, the purification steps are simplified, the purification process route is shortened, the time and cost are saved, the purification efficiency and benefits are improved, the purification yield is greater than 50% when the purity is higher than 90%, and the method is suitable for industrial production, and has significant effects.

Description

technical field [0001] The invention relates to a fusion expression of an antibacterial peptide and a purification method thereof, in particular to a fusion expression of a cecropin antibacterial peptide and a purification method thereof. Background technique [0002] With the overuse or even abuse of antibiotics, a series of drug-resistant bacteria have emerged, and even many "super bacteria" have appeared. Under this background, it is urgent to develop a new and efficient antibacterial agent to replace or Partially replace antibiotics. Antimicrobial peptides are a class of small molecular polypeptides produced by the host that can resist external microorganisms and eliminate mutant cells in the body. Its chemical essence is that the protein structure is decomposed into basic unit amino acids in the body, with no residual problems, no immunogenicity, and basically no drug resistance. The antimicrobial peptide itself not only has broad-spectrum antibacterial and bactericid...

AI Technical Summary

Problems solved by technology

The process of extracting antimicrobial peptides from animals and plants is complicated, the purification is difficult, the yield is low, and the production cost is high; the chemical synthesis of antimicrobial peptides also has disadvantages such as complex processes, poor activity, and high cost, which can only be studied in the laboratory, which is not suitable. Industrialized production; the expression of antimicrobial peptides by genetic engineering technology, which has the characteristics of high expression and high activity, has become a hot spot in the research of antimicrobial peptide production

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