Molecular specificity labeled primer for mutual discrimination of Amaranthus albus L., Amaranthus blitum L. and Amaranthus retroflexus L., and method

A technology of labeling primers and specificity, which is applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc. And other issues

Active Publication Date: 2020-04-03
FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these genomic DNA-based molecular markers are susceptible to genomic DNA degradation, because nuclear genomic DNA has fewer copies than chloroplast DNA and is more likely to be destroyed in processed medicinal materials, making identification by its molecular markers impossible

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular specificity labeled primer for mutual discrimination of Amaranthus albus L., Amaranthus blitum L. and Amaranthus retroflexus L., and method
  • Molecular specificity labeled primer for mutual discrimination of Amaranthus albus L., Amaranthus blitum L. and Amaranthus retroflexus L., and method
  • Molecular specificity labeled primer for mutual discrimination of Amaranthus albus L., Amaranthus blitum L. and Amaranthus retroflexus L., and method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] 1 instrument

[0026] PCR instrument (Eppendorf, model 5332), real-time quantitative PCR instrument (ABI7900), electrophoresis system (Beijing Liuyi Instrument Factory, model DYY-12), low-temperature refrigerated centrifuge (Eppendorf, model 5810R), gel imaging analyzer ( BIO-RAD ChemiDoc XRS), micropipette (Eppendorf).

[0027] 2 reagents

[0028] 2×CTAB extract, 1×TAE buffer, agarose (Promega Company), ethidium bromide (Fluka Company), TransStart®TopTaq DNA Polymerase (Beijing Quanshijin Company), chloroform, absolute ethanol, iso All propanol is of domestic analytical grade.

[0029] 3 materials

[0030] The experimental samples in this study were identified and collected by Wei Yicong of Fujian University of Traditional Chinese Medicine and collected from different origins: 10 samples of green amaranth, amaranth and retroflexus were collected from different origins in China.

[0031] method

[0032] 4.1 Genomic DNA extraction:

[0033]⑴ Take 5 g of fresh green...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a molecular specificity labeled primer for mutual discrimination of Amaranthus albus L., Amaranthus blitum L. and Amaranthus retroflexus L., and a method, and belongs to the technical field of biology. According to matK gene sequences of the Amaranthus albus L., the Amaranthus blitum L. and the Amaranthus retroflexus L., upstream specific forward primers AalF: 5'-TTTCAAAAAGGAATCGAAGATAA-3', AblF: 5'-CGAATCCATTTTTACGGAAACA-3' and AreF: 5'-GGGACTCAATTCTTCAGTAATACCA-3', and a downstream universal reverse primer matKR: 5'-AATAATGAGAAAGATTTCGGCAT-3' are designed. The specific primers are used for respectively building a multiplex PCR system and a fluorescent quantitative PCR system, and the fast and accurate mutual qualitative and quantitative discrimination among the Amaranthus albus L., the Amaranthus blitum L. and the Amaranthus retroflexus L. is realized.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a molecular specific marker primer and a method for mutual identification of green amaranth, amaranthus amaranthus and reflexa reflexa. Background technique [0002] The medicinal plant Amaranth ( Amaranthus albus L. ) The whole plant can be used as medicine, also often called "white amaranth". It has the effect of clearing away heat and detoxification. External application can cure sores, gnats, and insect bites. Amaranth ( Amaranthus blitum L.) The whole herb is used as a medicine for relieving pain, astringent, diuretic and antipyretic. Amaranthus retroflexus ( Amaranthus retroflexus L.) is mainly used to treat diarrhea, dysentery, hemorrhoid swelling, pain and bleeding embolism. The three are similar in shape and have similar distributions, often associated with each other, and are easy to be confused and misused. But they have different properties and medi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156
Inventor 魏艺聪车苏容卢伟
Owner FUJIAN UNIV OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap