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Composition for preventing and treating diabetic nephropathy and application thereof

A technology of diabetic nephropathy and composition, which is applied in the field of medicine and can solve problems such as impacts that have not been explored in depth

Pending Publication Date: 2020-04-21
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although studies in recent years have shown that Salvia miltiorrhiza and stems and leaves of Danshen have a good protective effect on diabetic nephropathy, most of the researches on the activity of the crude extracts of roots or stems and leaves of Salvia miltiorrhiza have not deeply explored the effect of the combination of different molecular components on DN. influences

Method used

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  • Composition for preventing and treating diabetic nephropathy and application thereof
  • Composition for preventing and treating diabetic nephropathy and application thereof
  • Composition for preventing and treating diabetic nephropathy and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Implementation example 1: based on HK-2, HBZY-1 cell model optimal salvia miltiorrhiza molecule compatibility ratio

[0014] 1 Instruments and materials

[0015] 1.1 Instrument

[0016] Forma seriesⅡwater jacket type CO 2 Incubator (Thermo Company); 1300series A2 ultra-clean bench (Thermo Company); PrimoStar inverted microscope (ZEISS Company), Auto VertA1 inverted fluorescence microscope; TOMY SX-500 autoclave (Nanjing Jitian Biotechnology Co., Ltd.) ; BWS-10 constant temperature water bath (Shanghai Yiheng Scientific Instrument Co., Ltd., ultrapure water preparation instrument (Milli-Q, Millipore, the United States); high-speed centrifuge (Allegra X-12R Centrifuge and Microfuge 22R Centrifuge, Backman company); MINI -4K microcentrifuge (Hangzhou Miou Instrument Co., Ltd.); Enspire multi-functional microplate reader (PerkinElmer, USA); pipette (Eppendorf); WGL-230B electric blast drying oven (Tianjin Test Instrument Co., Ltd. Company); 96-well cell culture plate, ce...

Embodiment 2

[0055] Implementation Example 2: Evaluation of the Biological Effects of Improving Diabetic Nephropathy Based on the Danshen Molecular Composition of the db / db Mouse Model

[0056] 1 Materials and reagents

[0057] 1.1 Experimental animals

[0058] Select 7-week-old male spontaneous type 2 diabetic and obese db / db mice, body weight (40±5) g; same genetic background db / m normal mice, body weight (21±2) g, both are BKS.Cg- Dock7 m+ / + Lepr db / Nju strain. The above animals were purchased from the Model Animal Research Institute of Nanjing University, animal license number: SCXK (Su) 2015-0001. All mice were kept in a barrier environment in the SPF experimental animal center of Nanjing University of Traditional Chinese Medicine. Replace bedding.

[0059] All animal experiment protocols were reviewed and approved by the Animal Ethics Committee of the Drug Safety Evaluation Research Center of Nanjing University of Traditional Chinese Medicine, and all experimental operations w...

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Abstract

The invention discloses a composition for preventing and treating diabetic nephropathy. According to the invention, an optimal compound combination for treating diabetic nephropathy is screened from salvia miltiorrhiza bunge through a large number of experiments; wherein the molar ratio of five molecules is (salvianolic acid B: rosmarinic acid : lithospermic acid: salvianic acid A: tanshinone IIA=1: 0.07: 0.04: 0.07: 0.05) and the molar ratio of three molecules is (salvianolic acid B: rosmarinic acid : lithospermic acid =1: 1.16: 0.02) have relatively good activity in HBZY-1 and HK-2 high glucose and hydrogen peroxide damage models, kidney injury caused by diabetes mellitus can be remarkably improved, and fasting blood glucose, triglyceride, urea nitrogen, creatinine, insulin, microalbuminuria, glutamic oxalacetic transaminase and glutamic-pyruvic transaminase levels of db / db mice with spontaneous diabetes mellitus are regulated; and pathological changes of kidney tissues are improved; and a good technical effect is achieved.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a molecular composition in salvia miltiorrhiza that can prevent and treat diabetic nephropathy and its application. Background technique [0002] Salvia miltiorrhiza is the dry root and rhizome of Salvia miltiorrhiza Bge., which is a plant of the genus Salvia in the Lamiaceae family. It was first recorded in "Shen Nong's Materia Medica", and was listed as the top grade. It has the effects of promoting blood circulation and removing blood stasis, dredging menstruation and relieving pain, cooling blood and eliminating carbuncle, clearing heart and eliminating troubles. Irregular menstruation, amenorrhea, upset and insomnia, accumulation of lumps, sores, swelling and pain. The chemical components in Danshen mainly include fat-soluble tanshinones and water-soluble salvianolic acids. More than 30 kinds of phenolic acids have been isolated from Salvia miltiorrhiza, mainl...

Claims

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Application Information

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IPC IPC(8): A61K31/343A61K31/192A61K31/216A61P13/12A61P3/10
CPCA61K31/343A61K31/192A61K31/216A61P13/12A61P3/10A61K2300/00
Inventor 宿树兰段金廒朱悦钱大玮郭盛项想徐卓
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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