Latex microspheres, application thereof and glycosylated hemoglobin detection kit

A technology of glycosylated hemoglobin and detection kits, which is applied in the direction of anti-animal/human immunoglobulin, biological testing, measuring devices, etc., can solve the problem of inaccurate testing of anemia samples, and achieve long-term storage and use, eliminate influence, and stabilize good sex effect

Active Publication Date: 2020-04-24
SONOSCAPE MEDICAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, anemia samples with Hb content <80g / L still cannot be accurately tested

Method used

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  • Latex microspheres, application thereof and glycosylated hemoglobin detection kit
  • Latex microspheres, application thereof and glycosylated hemoglobin detection kit
  • Latex microspheres, application thereof and glycosylated hemoglobin detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1: Preparation of the detection kit of the present invention

[0039] All components shown in Table 2 below were mixed at the concentrations shown below to prepare Reagent R1 and Reagent R2.

[0040] Table 2

[0041]

[0042] Detection method: Dilute the sample 100 times with hemolyzing agent, add 10uL sample, 260uL latex reagent R1, incubate at 37℃ for 30 seconds, then add antibody reagent R2, react at 37℃ for 3 minutes, and measure the absorbance value at 660nm. The percentage of HbA1c in the sample was calculated according to the standard curve of the percentage concentration of HbA1c.

Embodiment 2

[0043] Example 2: Preparation of the detection kit of the present invention

[0044] All components shown in Table 3 below were mixed at the concentrations shown below to prepare Reagent R1 and Reagent R2.

[0045] table 3

[0046]

[0047] Detection method: Dilute the sample 100 times with hemolyzing agent, add 10uL sample, 260uL latex reagent R1, incubate at 37℃ for 30 seconds, then add antibody reagent R2, react at 37℃ for 3 minutes, and measure the absorbance value at 660nm. The percentage of HbA1c in the sample was calculated according to the standard curve of the percentage concentration of HbA1c.

Embodiment 3

[0048] Example 3: Preparation of the detection kit of the present invention

[0049] All components shown in Table 4 below were mixed at the concentrations shown below to prepare Reagent R1 and Reagent R2.

[0050] Table 4

[0051]

[0052]

[0053] Detection method: Dilute the sample 100 times with hemolyzing agent, add 10uL sample, 260uL latex reagent R1, incubate at 37℃ for 30 seconds, then add antibody reagent R2, react at 37℃ for 3 minutes, and measure the absorbance value at 660nm. The percentage of HbA1c in the sample was calculated according to the standard curve of the percentage concentration of HbA1c.

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Abstract

The invention relates to the technical field of in-vitro diagnosis, and discloses latex microspheres, application thereof and a glycosylated hemoglobin detection kit. The invention provides the latexmicrospheres coupled with hemoglobin antibodies and the glycosylated hemoglobin detection kit containing the latex microspheres. Due to the addition of the latex microspheres, the glycosylated hemoglobin antibody cannot be adsorbed or connected to latex; the influence of hemoglobin content in different blood samples on glycosylated hemoglobin detection can be eliminated, the glycosylated hemoglobin content in anemia samples can be accurately detected, the incubation time is shorter, the stability is good, and long-term storage and use are facilitated.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnosis, in particular to a latex microsphere and its application and a glycosylated hemoglobin detection kit. Background technique [0002] Glycated hemoglobin (HbA1c) is the product of the combination of hemoglobin in red blood cells and blood sugar in human blood. The combination of blood sugar and hemoglobin to generate glycosylated hemoglobin is an irreversible reaction, which is proportional to the blood sugar concentration and lasts for about 120 days. Therefore, the blood sugar concentration between 120 days can be observed, which can reflect the blood sugar control of the patient in the past 8 to 12 weeks. Its characteristics determine that it has great significance in diabetes monitoring: (1) It is parallel to the blood sugar value. The higher the blood sugar, the higher the glycosylated hemoglobin, so it can reflect the level of blood sugar control; (2) the production is slow. Sinc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/18G01N33/72G01N33/577C08J7/12
CPCC07K16/18C08J7/12G01N33/577G01N33/723
Inventor 杨晓光刘玲
Owner SONOSCAPE MEDICAL CORP
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