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A Molecular Marker Co-segregated with Rice Photothermosensitive Genic Male Sterility Trait and Its Application

A nuclear-sensitive and male-sterile technology, applied in the field of plant molecular biology, can solve problems such as little understanding of molecular basis

Active Publication Date: 2021-12-24
HAINAN BOLIAN RICE GENE TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although many rice photothermosensitive genic male sterile genes have been mapped or cloned, the molecular basis for the regulation of rice photothermosensitive genic male sterility traits is still poorly understood

Method used

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  • A Molecular Marker Co-segregated with Rice Photothermosensitive Genic Male Sterility Trait and Its Application
  • A Molecular Marker Co-segregated with Rice Photothermosensitive Genic Male Sterility Trait and Its Application
  • A Molecular Marker Co-segregated with Rice Photothermosensitive Genic Male Sterility Trait and Its Application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Phenotypic and Genetic Analysis of Rice light tms4430 embodiment 1 Example mutation PTSGMS

[0043] Screening 1) tms4430 mutant

[0044] June 2013 93-11 10 kilograms radiation seeds get M0-generation cobalt-60. The radiation seeds planted in experimental plots Lingao County, Hainan Province, mature sub-species of plant closing, M1 generation materials total of about 6,500. The spring of 2014, many election of 3617 the amount of seed planted into the material M1 generation lines, each line 50 plant species. Respectively tillering, booting, heading, flowering, grain filling screening plant type, spike type, fertility, yield and other types of mutants were preserved and closed. Number 4430 in which the system performance is found that a mutant male sterility, is named tms4430.

[0045] Phenotypic 2) tms4430 Mutants

[0046] In cultivation conditions lingao positive quarter with iodine - potassium iodide solution (0.6% KI, 0.3% I 2 , W / w) of different grass pollen were staine...

Embodiment 2

[0049] Preliminary positioning light TMS4430 temperature sensitive male sterility gene of Example 2

[0050] A mixing tank using extreme trait analysis (bulked segregant analysis) and recessive population analysis (recessive-class analysis) of TMS4430 gene targeting. In the present MH63 parent tms4430 we constructed a mutant of F2 population, isolated in 169 sterile plants. Using the 301 pairs of SSR uniformly distributed on the screening of chromosomal markers 12 markers polymorphic between 93-11 and MH63, screened 64 pairs of polymorphic SSR markers, low polymorphism showed clear 63 and 93-11 recovery smaller difference in the genomic sequence. The F2 population were constructed fertile plants and sterile plants mixed pool of genes, polymorphic markers with the 64 pairs of the gene pool of the fertile and sterile gene pool analysis. The results indicated the presence of linkage SSR markers RM243 and RM023 and the mutant phenotype tms4430 on chromosome 1. Genotyping by-sterile pl...

Embodiment 3

[0051] Example 3 Rice Fine Mapping light of temperature sensitive male sterility gene TMS4430

[0052] Based on the above results of the rough positioning, recessive population analysis (recessive-class analysis) for fine positioning. Using markers linked heteroaryl selected tms4430 F2 population developed a laminated F3 plant population, comprising 3827 plant, wherein the isolated 952 sterile plants. Meanwhile, between the marks and the RM10544 RM10495 further developed three polymorphic markers. Wherein the primer for detecting the molecular marker Indel14270 as shown in SEQ ID NO.1-2; molecular marker for detecting Indel14340 primer as shown in SEQ ID NO.3-4; means for detecting the primer shown in SEQ M3193-6 FIG IDNO.5-7.

[0053] Using these markers individually detected F3 populations isolated genotype sterile plants, found Indel14270 sterile plants only mark an expanded band of 305bp ( image 3 ); Indel14340 mark only in the expanded band of 132bp ( Figure 4 ); Only the numer...

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Abstract

The invention provides a molecular marker that is co-separated with the photothermosensitive nuclear male sterility trait of rice and its application, and belongs to the technical field of genetic engineering. In the present invention, tms4430 is finely positioned on rice chromosome 1 by the method of map-based cloning, and is located in the 237.39Kb segment between molecular markers RM10495 and RM10519, and the gene is co-segregated with markers Indel14270, Indel14340 and M3193-6. The tms4430 homozygous mutant showed a photo-temperature-sensitive phenotype of long-day high-temperature sterility and short-day low temperature fertilization. The fine mapping of tms4430 and the development of co-segregation linkage markers provide a new sterile gene source and molecular assisted selection method for the breeding of two-line CMS lines in rice, and have great application potential in the breeding of two-line CMS lines .

Description

Technical field [0001] The present invention is in the field of plant molecular biology, particularly applications involving molecular markers and a co-isolated nuclear male sterility PTGMS Hybrid Rice. Background technique [0002] Rice is the staple food for more than half the world's population, will help increase rice production to help mankind to rid itself of the problem of hunger. The use of hybrid rice heterosis in rice, the yield, resistance and adaptability of rice has been greatly enhanced. Mr. Yuan Longping made from the last century 60's male sterile hybrid rice production began to use rice, Chinese hybrid rice development experience from the three-line to two-line hybrid rice. Using hybrid rice sterile lines Cytoplasmic sterility hybrid rice seed, and the two-line hybrid rice by the light TGMS incubated for Hybrid Seed Production. Relative to the sterile lines, Two Sterile broad-based recovery, with the group more freedom, greatly simplifying the process of producti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/29C12N15/11C12Q1/6895
CPCC07K14/415C12Q1/6895C12Q2600/13C12Q2600/156
Inventor 唐杰龙湍李佳林刘昊韩晓斌曾翔吴永忠黄培劲
Owner HAINAN BOLIAN RICE GENE TECH CO LTD