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A compound Chinese herbal medicine preparation for inhibiting the growth of cyanobacteria and its preparation method

A Chinese herbal medicine, compound technology, applied in the directions of plant growth regulators, botanical equipment and methods, biocides, etc., can solve problems such as adverse effects on the physiological health of aquatic animals, damage to the ecological environment of water bodies, and large microbial aerobic capacity. Spirulina blooms, alleviating toxic effects, increasing immunity

Active Publication Date: 2021-08-03
GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing problems include: (1) Adsorbents and flocculants such as clay, sodium alginate, activated carbon, etc., have problems such as large amount of addition and easy recurrence; (2) Chemical drugs such as chlorine-containing preparations, copper sulfate, glutaraldehyde, etc. , there are the problems of high toxicity of chemical agents, long residual time, and difficulty in degrading, which will damage the original ecological environment of the water body, have adverse effects on the physiological health of aquatic animals, and even affect human health; (3) the existence of various microorganisms is harmful The problem of high oxygen content and difficult colonization

Method used

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  • A compound Chinese herbal medicine preparation for inhibiting the growth of cyanobacteria and its preparation method
  • A compound Chinese herbal medicine preparation for inhibiting the growth of cyanobacteria and its preparation method
  • A compound Chinese herbal medicine preparation for inhibiting the growth of cyanobacteria and its preparation method

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Experimental program
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Effect test

Embodiment 1

[0029] 1. Source of algae species:

[0030] The serial number is FACHB-905, which was purchased from the freshwater algae library of the Institute of Hydrobiology, Chinese Academy of Sciences. Before the experiment, the algae should be cultured to logarithmic phase (about 1.0 × 10 7 cfu / ml) for the experiment.

[0031] 2. Preparation method of compound Chinese herbal medicine preparation for inhibiting the growth of cyanobacteria

[0032] A. Pulverize Astragalus, Banlangen, Cork, and Gardenia to 20 meshes, put them into 80-120 mesh non-woven filter bags and put them in each extraction pot, add sterile pure water with 3 times the quality of Chinese herbal medicine, and heat to Soak at 40°C for 30 minutes.

[0033] B. Add sterile pure water with 8 times the mass of each Chinese herbal medicine, boil for 40 minutes, and filter out the extract. Repeat this step 3 times, and combine the extracts (respectively combine the extracts extracted from each traditional Chinese medicine...

Embodiment 2

[0043] 1. Source of algae species:

[0044] The serial number is FACHB-905, which was purchased from the freshwater algae library of the Institute of Hydrobiology, Chinese Academy of Sciences. Before the experiment, the algae should be cultured to logarithmic phase (about 1.0 × 10 7 cfu / ml) for the experiment.

[0045] 2. Preparation method of compound Chinese herbal medicine preparation for inhibiting the growth of cyanobacteria

[0046] A. Pulverize Astragalus, Banlangen, Cork, and Gardenia to 20 meshes, put them into 80-120 mesh non-woven filter bags and put them in each extraction pot, add sterile pure water with 3 times the quality of Chinese herbal medicine, and heat to Soak at 40°C for 30 minutes.

[0047] B. Add sterile pure water with 8 times the mass of each Chinese herbal medicine, boil for 40 minutes, and filter out the extract. Repeat this step 3 times, and combine the extracts (respectively combine the extracts extracted from each traditional Chinese medicine...

Embodiment 3

[0057] 1. Source of algae species:

[0058] The serial number is FACHB-905, which was purchased from the freshwater algae library of the Institute of Hydrobiology, Chinese Academy of Sciences. Before the experiment, the algae should be cultured to logarithmic phase (about 1.0 × 10 7 cfu / ml) for the experiment.

[0059] 2. Preparation method of compound Chinese herbal medicine preparation for inhibiting the growth of cyanobacteria

[0060] A. Pulverize Astragalus, Banlangen, Cork, and Gardenia to 20 meshes, put them into 80-120 mesh non-woven filter bags and put them in each extraction pot, add sterile pure water with 3 times the quality of Chinese herbal medicine, and heat to Soak at 40°C for 30 minutes.

[0061] B. Add sterile pure water with 8 times the mass of each Chinese herbal medicine, boil for 40 minutes, and filter out the extract. Repeat this step 3 times, and combine the extracts (respectively combine the extracts extracted from each traditional Chinese medicine...

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Abstract

The invention discloses a compound Chinese herbal medicinal preparation for inhibiting the growth of cyanobacteria and a preparation method thereof. Traditional Chinese medicine raw materials: Astragalus, Radix Radix, Phellodendron, and Gardenia; astragalus, Radix Radix, Phellodendron, and Gardenia are crushed, extracted, decocted, and the filtrate is collected by filtration, and then concentrated to obtain extracts of Astragalus, Radix Radix, Phellodendron, and Gardenia respectively ; A compound Chinese herbal medicine preparation for inhibiting the growth of blue-green algae, in terms of mass fraction, including 10-15% of astragalus extract, 8-13% of Radix isatidis extract, 3-8% of Cortex Phellodendri extract, 3-8% of gardenia extract, 56% of water -76%. The invention proves through experiments that it can kill cyanobacteria, reduce the density of cyanobacteria cells, and effectively prevent and control cyanobacteria blooms. The invention can effectively solve the water bloom caused by the explosive growth of cyanobacteria, alleviate the poisonous effect of algae toxins on cultured animals, improve the immunity of animals, and improve the survival rate of cultured animals. Therefore, it has broad application prospects.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a compound Chinese herbal medicine preparation for inhibiting the growth of cyanobacteria and a preparation method. Background technique [0002] In recent years, my country's economy has developed rapidly, and the discharge of domestic sewage, industrial wastewater, and aquaculture wastewater has resulted in a large accumulation of nitrogen and phosphorus in water bodies and natural water bodies, resulting in eutrophication of water bodies. The eutrophication of the water body leads to the overgrowth of algae and causes the outbreak of algal blooms. The algal blooms not only change the water color, landscape and water quality, but also produce algal toxins that endanger the health of farmed animals and even human health. This has become a global problem, causing people of general attention. At present, algae control technologies mainly include (1) physical method: isola...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N65/36A01N65/08A01N65/20A01P13/00
CPCA01N65/00A01N65/08A01N65/20A01N65/36
Inventor 许国焕魏逸峰张丽袁满华李志朋苏奎英
Owner GUANGDONG INST OF MICROBIOLOGY GUANGDONG DETECTION CENT OF MICROBIOLOGY
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