An anti-tumor nano-preparation and its application in targeted therapy of malignant tumors

A technology of nano-preparation and targeted therapy, which is applied in the field of anti-tumor nano-preparation and its application in targeted therapy of malignant tumors. It can solve the problems of few and can not achieve the optimal effect, and achieve small trauma area and non-toxicity. Side effects, good biocompatibility

Active Publication Date: 2022-05-27
JIANGXI HERBFINE HI TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage is that photothermal therapy alone cannot achieve the optimal effect, and highly targeting antineoplastic drugs to the tumor site is a major problem to be solved.
At present, there are not many studies on the use of phospholipids to target tumor sites, especially the research and application of targeting tumor sites and combined photothermal therapy, and there are few studies on highly targeted delivery of drugs to tumor sites in combination with chemotherapy. The present invention Made a further contribution in this regard and successfully prepared a new type of high-efficiency anti-tumor drug

Method used

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  • An anti-tumor nano-preparation and its application in targeted therapy of malignant tumors
  • An anti-tumor nano-preparation and its application in targeted therapy of malignant tumors
  • An anti-tumor nano-preparation and its application in targeted therapy of malignant tumors

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The preparation steps of polydopamine nanoparticles and polydopamine nanoparticle core-loaded epirubicin nano-formulations are as follows:

[0047] S1: Take 1 g of L-cysteine ​​and 6 ml of 25% ammonia water and add them to 260 ml of 30% ethanol solution, stir and mix evenly, take a water bath at 35 °C for 10 min in the dark, and add 0.26 mol / 1 dopamine hydrochloride 40 to the mixed solution. m1, continue to stir for 24h to obtain PDA-NPS;

[0048] S2: after dialyzing the PDA-NPS dialysis tube obtained in step S1, continue to dilute it by 2 times with water to obtain solution A;

[0049] S3: Add the adjuvant drug loading agent, aqueous binder, and epirubicin (EPI) in a mass ratio of 12:4:1 to 2 ml of methanol in sequence to fully dissolve to obtain solution B;

[0050] S4: Take the solution B of step S3 and slowly add it dropwise to the solution A of step S2, shake while dripping, fully mix and place the mixture in a 37 °C water bath for 30 min; after the water bath, di...

Embodiment 2

[0053] The preparation steps of polydopamine nanoparticles and polydopamine nanoparticle core-loaded epirubicin nano-formulations are as follows:

[0054] S1: Take 1 g of L-cysteine ​​and 6 ml of 25% ammonia water and add them to 260 ml of 30% ethanol solution, stir and mix evenly, take a water bath at 0 °C for 5 min in the dark, and add 0.26 mol / l dopamine hydrochloride to the mixed solution 60 ml, continue stirring reaction 72h, promptly obtain PDA-NPS;

[0055] S2: after dialyzing the PDA-NPS dialysis tube obtained in step S1, continue to dilute it with water 6 times to obtain solution A;

[0056] S3: Add the adjuvant drug loading agent, aqueous binder, and epirubicin (EPI) in a mass ratio of 6:10:1 to 2 ml of methanol in sequence to fully dissolve to obtain solution B;

[0057] S4: Take the solution B of step S3 and slowly add it dropwise to the solution A of step S2, shake while dripping, fully mix and place the mixture in a water bath at 45 °C for 25 min; after the wate...

Embodiment 3

[0060] The preparation steps of polydopamine nanoparticles and polydopamine nanoparticle core-loaded epirubicin nano-formulations are as follows:

[0061] S1: Take 1 g of L-cysteine ​​and 6 ml of 25% ammonia water and add them to 260 ml of 30% ethanol solution, stir and mix evenly, take a water bath at 40 °C for 7 min in the dark, and add 0.26 mol / l dopamine hydrochloride to the mixed solution 80 ml, continue to stir and react for 0.5 h to obtain PDA-NPS;

[0062] S2: after dialyzing the PDA-NPS dialysis tube obtained in step S1, continue to dilute it with water 8 times to obtain solution A;

[0063] S3: Add the adjuvant drug loading agent, aqueous binder, and epirubicin (EPI) in a mass ratio of 1:2:1 to 2 ml of methanol in sequence to fully dissolve to obtain solution B;

[0064] S4: Take the solution B of step S3 and slowly drop it into the solution A of step S2, shake while dripping, fully mix and place the mixture in a 30 ℃ water bath for 30 min; after the water bath, dia...

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Abstract

The invention discloses an anti-tumor nano-preparation and its application in targeted treatment of malignant tumors. Polydopamine nanoparticles are used as a photothermal conversion material to prepare a bio An anti-tumor nano-preparation with good compatibility and almost no cytotoxicity; at the same time, the therapeutic effect of killing tumor cells can be achieved by combining photothermal therapy. After the tail vein injection of the preparation of the present invention into the body of BALB / C female mice inoculated with 4T1-luc cells, the therapeutic effect of photothermal therapy is significantly better than that of conventional treatment, and the treatment method has a small wound area and almost no toxic side effects. The dosage is reduced, and the pain is reduced compared with surgical treatment.

Description

technical field [0001] The invention relates to the research and application of chemotherapy combined with photothermal treatment of malignant tumors, in particular to an anti-tumor nano preparation and its application in targeted treatment of malignant tumors. Background technique [0002] In recent years, photothermal therapy has received extensive attention in the academic community and has made good progress. The treatment principle is that photothermal materials absorb laser light and convert light energy into heat energy to rapidly heat up the tumor site, making the temperature exceed the tolerance temperature of cancer cells. 50°C to kill cancer cells. The combined treatment of malignant tumors with various methods has significantly increased the effect to a certain extent. The use of photothermal therapy combined with chemotherapy to treat tumors has achieved good results to a large extent. The good application prospect has become a hot research topic. Noble metal ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/24A61K41/00A61K31/704A61P35/00A61K49/00
CPCA61K9/5123A61K9/5146A61K41/0052A61K31/704A61P35/00A61K49/0008A61K2300/00
Inventor 李翔张婧邹茜
Owner JIANGXI HERBFINE HI TECH
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