Inorganic-organic hybrid Keggin type polyoxometallate compound as well as preparation method and antibacterial application thereof

A technology of polyoxometalates and organic hybridization, which is applied in organic chemistry, antibacterial drugs, organic active ingredients, etc., can solve the problems of instability of pure inorganic substances, limit cell penetration, etc., and achieve enhanced stability and Penetration ability, excellent antibacterial performance, strong stability effect

Active Publication Date: 2020-05-08
HENAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, pure inorganics exhibit instability under physiological co

Method used

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  • Inorganic-organic hybrid Keggin type polyoxometallate compound as well as preparation method and antibacterial application thereof
  • Inorganic-organic hybrid Keggin type polyoxometallate compound as well as preparation method and antibacterial application thereof
  • Inorganic-organic hybrid Keggin type polyoxometallate compound as well as preparation method and antibacterial application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0034] Example 1

[0035] A method for preparing an inorganic-organic hybrid Keggin type polyoxometalate complex includes the following steps:

[0036] Co(NO 3 ) 2 ·6H 2 O (0.189 g, 1.3 mmol), Na 2 MoO 4 ·2H 2 O (0.48 g, 2.0 mmol), EDTMP (0.35 g, 0.8 mmol) and 2-acetylpyrazine thiosemicarbazone (0.097 g, 0.5 mmol) were added to 60 mL of water and methanol (volume ratio 2:1) In the mixed solvent, stir for 40 minutes until the dissolution is complete. Use dilute hydrochloric acid to adjust the pH value between 2.5 and 3.0, then transfer it to the reaction flask, put it in a polytetrafluoroethylene lining and keep it at 130°C for 3 days, and cool At room temperature, wash with water and dry to obtain black quadrilateral crystals, which are composites.

[0037] The above-mentioned inorganic-organic hybrid Keggin polyoxometalate belongs to the monoclinic crystal system, C2 / c Space group, its unit cell parameters are: a = 29.1829(13) Å, b = 16.5031(7) Å, c = 18.9095(9) Å, α = 90°, β ...

Example Embodiment

[0046] Example 2

[0047] Evaluation method of antibacterial effect 1 Disc diffusion method:

[0048] With DMSO as the blank control group, the test was carried out on agar plates to study the antibacterial activity of the material. Before the test, the complex was diluted with DMSO in a gradient (1 mg / mL, 0.5 mg / mL, 0.25 mg / mL, 0.125 mg / mL, 0.0625 mg / mL, 0.03125 mg / mL...). All materials used in the testing process were autoclaved, and the nutrient agar plate (NA) was sterilized by ultraviolet radiation for 1 hour. Will 10 7 CFU / mL Staphylococcus aureus ( S. aureus ), Bacillus subtilis ( B. subtilis ) And 10 6 CFU / mL Escherichia coli ( E. coli ) And Agrobacterium tumefaciens ( A. tumefaciens ) Fresh bacteria solution was evenly spread on the nutrient agar (NA) plate, and then punched, the prepared complex was sucked into the agar plate wells, and then sealed, the culture was incubated at 37°C for 16 hours , Finally, measure the zone of inhibition (ZOI) of all samples, and rec...

Example Embodiment

[0053] Example 3

[0054] Time killing experiment evaluation 2 colony forming unit (CFU) method:

[0055] The bactericidal ability of the compound was studied by the plate coating culture method. Serially dilute the E. coli and Staphylococcus aureus solution to 10 5 CFU / mL, and prepare a 1 mg / mL complex solution. Incubate the same volume of the inoculum solution and the complex solution for different times at 37°C. The bacterial solution without complexes is used as a control experiment. The mixed solution of the different culture time is evenly spread on the nutrient agar (NA) plate, each time The measurement was repeated three times and cultured in a 37°C constant temperature incubator for 16 hours. The experiment continued until almost no colonies were observed. The experimental results show as Picture 9 with 10 . Bacterial cell survival rate (%)= (number of colonies in the experimental group / number of colonies in the control group)×100%.

[0056] Picture 9 with Picture 1...

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Abstract

The invention discloses an inorganic-organic hybrid Keggin type polyoxometallate compound; the chemical formula of the compound is [Co(L)2]3[PMo12O40], and in the formula, HL is 2-acetylpyrazine thiosemicarbazone; the compound belongs to a monoclinic system and a C2/c space group, and cell parameters comprises a=29.1829(13) angstroms, b=16.5031(7) angstroms, c=18.9095(9) angstroms, alpha=90 degrees, beta=103.465(1) degrees and gamma=90 degrees. According to the compound, the 2-acetylpyrazine thiosemicarbazone and the POM are self-assembled together; the stability and penetrability of the POM are enhanced, the POM has excellent antibacterial performance, and the obtained compound can play a considerable antibacterial role in the biological and food industries and other application fields, for example, the compound can be used as an antibacterial material and an antibacterial reagent or used for preparing antibacterial drugs and the like.

Description

technical field [0001] The invention belongs to the technical field of inorganic-organic functional polyacid. It specifically relates to a combination of 2-acetylpyrazine thiosemicarbazone compound with good bactericidal effect and POM with high redox, anticancer and antimicrobial activities to form an inorganic-organic hybrid Keggin-type polyoxometallic acid salt complex. The complex self-assembles 2–acetylpyrazine thiosemicarbazone with POM, which not only enhances the stability and penetration ability of POM, but also makes it have excellent antibacterial properties, which can be used in biological and food industries and other applications. Fields exert a considerable antibacterial effect. Background technique [0002] Bacterial infections are a serious problem that can threaten human health and the growth of plants and animals. Overuse or even misuse of antibiotics can make bacteria resistant to antimicrobial drugs. Today, millions of people all over the world die f...

Claims

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Application Information

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IPC IPC(8): C07D241/12A61K31/4965A61P31/04A61K33/24A61K47/55
CPCC07D241/12A61P31/04A61K33/24A61K47/55
Inventor 李明雪赵萌刘泰宇方颜刘静
Owner HENAN UNIVERSITY
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