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High-precision urine iodine detection method

A test method and high-precision technology, applied in the field of urine iodine test, can solve the problems of low precision and accuracy, expensive equipment, threats to users and the environment, achieve high precision and accuracy, improve precision, and avoid measurement Effects of Insufficient Precision and Sensitivity

Active Publication Date: 2020-05-19
北京中西医结合医院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Among them, inductively coupled plasma mass spectrometry has the advantages of fast analysis speed, wide linear range, and less matrix interference. However, the instruments used in this method are expensive, and the detection and operation costs are high. The equipment is simple and the detection cost is low, but it has strict requirements on the detection conditions, and the actual sodium arsenite used is highly toxic, which poses a great threat to users and the environment
[0004] The patent with publication number CN110006881A provides a method for detecting iodine content in urine, which uses low concentration of 3,3',5,5'-tetramethylbenzidine and peracetic acid as the color reaction system instead The traditional arsenic-cerium color development system has less environmental pollution; however, the color change of the display reaction system provided by the invention is small, and it is difficult to detect trace changes in iodine content, and the sensitivity is low, which leads to the use of this method for urine iodine detection. There are problems of relatively low precision and accuracy in detection, it is difficult to achieve high-precision detection of urinary iodine, and the application is limited

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Experimental program
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Embodiment 1

[0061] The present embodiment provides a high-precision urine iodine test method, comprising the following steps:

[0062] S1, 267mg N-bromosuccinimide is dissolved in 100mL deionized water, the preparation concentration is the N-bromosuccinimide solution of 0.015mol / L; Add 100 μ L concentration to 4mL urine sample to be tested 2mol / L of hydrochloric acid and 60 μL of the N-bromosuccinimide solution are fully mixed and left to stand at room temperature for 2 minutes, so that the iodide ions in the urine sample to be tested are fully oxidized to iodate ions to obtain Solution A;

[0063] S2. Dissolve 9 g of oxalic acid in 100 mL of deionized water to prepare an oxalic acid solution with a concentration of 1 mol / L; add 520 μL of the oxalic acid solution to the solution A obtained in step S1, mix well and let stand at room temperature for 5 minutes. Completely react the remaining N-bromosuccinimide in the solution A to obtain solution B;

[0064] S3. Dissolve 1.5 g of tetrabuty...

Embodiment 2~15

[0083] Examples 2-15 respectively provide a high-precision urine iodine test method. Compared with Example 1, the difference is that the concentration of the reagent used in steps S1-S3 is adjusted, and the remaining steps are consistent with Example 1.

[0084] Wherein, embodiment 2~4 has only adjusted the concentration of N-bromosuccinimide solution in step S1, and the concentration of the N-bromosuccinimide solution corresponding to embodiment 2~4 is respectively 0.005mol / L, 0.01mol / L and 0.02mol / L. The corresponding absorbance of the urine sample to be measured is compared in embodiment 2 ~ 4 and embodiment 1, obtains the corresponding absorbance change curve when the concentration of N-bromosuccinimide solution is different, as image 3 shown.

[0085] Depend on image 3 It can be seen that along with the increase of the concentration of N-bromosuccinimide solution, the absorbance value of the urine sample extract increases first and then decreases. When the concentrat...

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Abstract

The invention discloses a high-precision urine iodine detection method which comprises the following steps: by taking N-bromosuccinimide as an oxidizing agent, oxidizing iodide ions in urine into iodate ions, introducing tetrabutylammonium iodide to react with the iodate ions, and forming an ion association compound by utilizing the electrostatic attraction effect between tetrabutylammonium cations and triiodide anions; and the ionic association compound is subjected to extraction and absorbance detection to establish a linear relationship between the iodine ion concentration and absorbance, thereby implementing high-precision detection on urine iodine. By means of the mode, the urine iodine can be safely and conveniently subjected to high-precision detection, the detection method is simple, convenient and easy to implement, high in detection speed and low in detection cost, the detection process has high sensitivity, precision and accuracy, high-precision detection of the urine iodinecan be achieved, the requirements of practical application can be met, and high application value is achieved.

Description

technical field [0001] The invention relates to the technical field of urine iodine testing, in particular to a high-precision urine iodine testing method. Background technique [0002] Iodine is an essential component for the synthesis of thyroid hormones. Insufficient or excessive iodine intake by the body will affect thyroid function, cause thyroid damage, and lead to thyroid diseases. About 90% of the iodine ingested by the human body is excreted through the urine after being absorbed by the gastrointestinal tract. The iodine content in the urine can roughly reflect the iodine intake and the iodine content in the blood. Therefore, urinary iodine is an important indicator for monitoring and evaluating the iodine nutrition level of the human body. Accurate detection of urinary iodine is of great significance for the prevention and treatment of related diseases, medical care and clinical diagnosis and treatment. [0003] Due to the low content of iodine in urine, complex m...

Claims

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Application Information

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IPC IPC(8): G01N21/31G01N21/01
CPCG01N21/01G01N21/31
Inventor 薛原
Owner 北京中西医结合医院
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