Application of inhibitor of nlrp3 inflammasome in preparation of medicine for treating pituitary adenoma and medicine for treating pituitary adenoma
An inflammasome and inhibitor technology, which can be used in anti-tumor drugs, drug combinations, and medical preparations containing active ingredients, etc. Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0041] Significant activation of the pituitary NLRP3 inflammasome in estradiol-induced prolactinoma rats.
[0042] 1. Establish a prolactinoma model in rats.
[0043] SD rats were purchased from Hubei Experimental Animal Center, and 20 SD rats were randomly divided into normal group and model group, with 10 rats in each group. Rats in the model group were injected intraperitoneally with estradiol benzoate injection (purchased from Sichuan Jinke Pharmaceutical Co., Ltd., batch number 20170702), with a dose of 20 mg / kg, injected once every 5 days, and continuously injected for 50 days; The same dose of normal saline was injected intraperitoneally.
[0044]2. MRI detection and appearance observation of rat pituitary gland.
[0045] MRI detection and appearance map reference figure 1 shown by figure 1 It can be seen that compared with the normal control group rats, the pituitary gland volume of rats with prolactinoma induced by estradiol injection was significantly increased. ...
Embodiment 2
[0061] This example demonstrates that the expression of PRL significantly increases after the activation of NLRP3 inflammasome in pituitary tumor GH3 cells.
[0062] GH3 cells were purchased from Beijing Beina Chuanglian Biotechnology Research Institute, LPS was purchased from sigma company, and MSU was purchased from sigma company. When the confluence of GH3 cells after passage was 70-80%, three 6-well plates were planted (repeat 3 each time), add 1mL of cell solution to each well, add 1mL of culture medium, put them into a carbon dioxide incubator and cultivate for 24 hours, absorb 1mL of medium from each well and discard, add stimulators respectively, and the stimulators in the 6-well plate The order of adding samples is shown in Table 1 below.
[0063] Table 1 Sampling order.
[0064]
[0065] After adding stimulators and culturing for 24 hours, the GH3 cells in each group were extracted from cell lysates for Western blot detection.
[0066] Western blot test results ...
Embodiment 3
[0068] This example demonstrates that the NLRP3 inflammasome inhibitor MCC950 reduces the expression of PRL in GH3 cells by inhibiting the activation of NLRP3 inflammasome. MCC950 was purchased from MedChemexpress.
[0069] After GH3 cells were plated, stimulators and MCC950 were added. When the fusion degree of the passaged GH3 cells is 70-80%, plant three 6-well plates (repeated 3 times), add 1 mL of cell solution to each well, add 1 mL of medium, and culture in a carbon dioxide incubator for 24 After 1 hour, suck out 1 mL of medium in each well and discard, add LPS solution and Ni solution respectively, and add different concentrations of MCC950 solution after 6 hours, the sequence of adding reagents is shown in Table 2 below.
[0070] Table 2 Reagent loading order.
[0071]
[0072] After adding MCC950 and culturing for 24 hours, the lysate protein was extracted from GH3 cells in each group for Western blot detection.
[0073] Western blot detection results of GH3 ce...
PUM
Login to view more Abstract
Description
Claims
Application Information
Login to view more - R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap