Detection reagent for genotype detection of SNP sites, application of detection reagent in preparation of corresponding kit and kit

Abstract

The invention provides a detection reagent for genotype detection of SNP sites, application of the detection reagent in preparation of a corresponding kit and the kit. The detection reagent includes aprimer group, wherein the primer group includes a No. 1 primer pair for specific amplification of the rs2239063 site of the CACNA1C gene so as to obtain a first amplification product, a No. 2 primerpair for specific amplification of the rs16921385 site of the SLC1A1 gene so as to obtain a second amplification product and a No. 3 primer pair for specific amplification of the rs17022006 site of the CNTN4 gene so as to obtain a third amplification product, the nucleotide sequences of an upstream primer and downstream primer in the No. 1 primer pair are shown in SEQ ID NO. 1 and SEQ ID NO. 2, and the nucleotide sequences of an upstream primer and downstream primer in the No. 2 primer pair are shown in SEQ ID NO. 3 and SEQ ID NO. 4; and the nucleotide sequences of an upstream primer and downstream primer in the No. 3 primer pair are shown in SEQ ID NO. 5 and SEQ ID NO. 6.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to a detection reagent for SNP locus genotype detection, the use of the detection reagent in preparing a kit for medication guidance for schizophrenia patients, the corresponding kit and its use method . Background technique [0002] Mental illness has become a highly prevalent disease in the world. According to statistics, more than 17% of Chinese adults suffer from depression, schizophrenia and other mental illnesses (Phillips.2009). Schizophrenia (SCZ) is one of the most common and serious mental illnesses, and it is prevalent in all populations around the world. Currently, about 1% of the world's population suffers from schizophrenia (Muesser.2004). Schizophrenia not only brings great suffering to the patients themselves and their families, but also brings heavy economic burdens to families, medical care and the whole society. In the Symposium on the Burden of Disease i...

AI Technical Summary

Problems solved by technology

Among them, the first-generation sequencing in the gene sequencing method is the gold standard for genotyping. This method is suitable for high-throughput multi-site detection, but it is time-consuming, complicated to operate, requires high operators and low sensitivity, and is prone to crossover between samples. Polluted and not suitable for rapid clinical testing; and the amount of data obtained by sequencing is large, a lot of information is useless to patients, and result interpretation also requires some professional knowledge, and the cost is relatively high

Denaturing high performance liquid chromatography has the advantages of high automation and high throughput, but the equipment is expensive and not suitable for general medical institutions

The high-resolution melting curve method is fast, simple, economical and practical, but it has special requirements for equipment, and it needs to be installed with special software and a temperature-sensitive machine before it can be used. There are certain difficulties in clinical promotion.

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