Chemiluminiscence kit and method for detecting trace haptoglobin in urine

Abstract

The invention discloses a chemiluminiscence kit for detecting trace haptoglobin in urine. The chemiluminiscence kit is used for detecting the trace haptoglobin in urine by adopting a double monoclonalantibody sandwich method; the chemiluminiscence kit comprises a chemiluminiscence plate, an enzyme-labeled monoclonal antibody solution, a standard substance solution and a luminescent substrate; thechemiluminiscence plate is coated with a diluted monoclonal antibody 5C4 and is sealed by a confining liquid; the enzyme-labeled monoclonal antibody solution is prepared from a monoclonal antibody HRP-1A2 labeled by HRP and an antibody diluent; and the monoclonal antibody 5C4 and the monoclonal antibody 1A2 are monoclonal antibodies capable of recognizing human natural Hp molecular structure epitopes. According to the chemiluminiscence reagent, the double monoclonal antibody sandwich method is adopted to detect a content of Hp in the urine; and the monoclonal antibodies 5C4 and 1A2 are used as double single antibodies, the pair of monoclonal antibodies can specifically recognize human Hp molecular structure epitopes, structural epitope antibodies can specifically detect non-denatured Hp molecules, detection accuracy is high, and a bias value is-6.78%-10.12%.

Description

technical field [0001] The invention relates to a chemiluminescent reagent kit and method for detecting trace amounts of haptoglobin in urine, belonging to the technical field of biomedical diagnosis. Background technique [0002] Haptoglobin (Hp), also known as haptoglobin, is widely present in serum and other body fluids of humans and various mammals. In CAM electrophoresis and agarose gel electrophoresis, haptoglobin is located in the α2 zone, and there are two pairs of peptide chains (α chain and β chain) in the molecule to form a tetramer of α2β2. The molecular structure of Hp is controlled by genetics, and there are two different alleles Hp1 and Hp2 in humans. Thus, three Hp genotypes were formed: Hp1-1, Hp2-1 and Hp2-2. [0003] The main function of haptoglobin is to combine with free hemoglobin to form a complex and present it to the monocyte-macrophage system for processing by combining with CD163 molecules, so as to remove free hemoglobin in the blood circulation...

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Problems solved by technology

However, this detection reagent also has obvious deficiencies: (1) the detection efficiency is low and requires a long detection time; (2) the detection antibody is a polyclonal antibody, which has poor stability, resulting in poor detection consistency between different batches of reagents; (3) it is not compat

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