Preparation method of photonic crystal microspheres based on multi-shell nanoparticles
A technology for photonic crystal microspheres and nanoparticles, which is used in the preparation of microspheres, biochemical equipment and methods, nanotechnology for materials and surface science, etc. problem, to achieve the effects of excellent color contrast and brightness, simple preparation method, and high biomedical research value
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Embodiment 1
[0031] Construction of photonic crystal microspheres based on graphene oxide (GO) and polydopamine (PDA)-wrapped multi-shelled silica nanoparticles and structural color comparison of photonic crystal microspheres with different nanoparticle compositions
[0032] 1. Preparation of PDA-wrapped silica nanoparticles: prepare the washed unmodified silica nanoparticles into a dispersion with a concentration of 0.1g / mL; the total volume of the entire reaction system in this step is 10mL, including 5mL concentration Tris base of 0.1mol / L, 1.47mL of hydrochloric acid with a concentration of 0.1mol / L, 2mL of silica nanoparticle dispersion, and the rest is dopamine solution (to ensure that the dosage of dopamine in the entire system is 20mg). Put 10mL of the mixed solution in a glass bottle, and place the whole system on a magnetic stirrer to mix and react for 10h. During the reaction, it can be observed that the originally clear and transparent reaction system turns into a dark gray opaq...
Embodiment 2
[0038] Based on molybdenum disulfide (MoS 2 ) and polydopamine (PDA)-wrapped multi-shell polystyrene (PS) nanoparticles to construct photonic crystal microspheres
[0039]1. Preparation of PDA-wrapped PS nanoparticles: Prepare the washed unmodified PS nanoparticles into a dispersion with a concentration of 0.1g / mL; the total volume of the entire reaction system in this step is 10mL, including 5mL with a concentration of 0.1mol / mL L of Trisbase, 1.47 mL of hydrochloric acid with a concentration of 0.1 mol / L, 2 mL of PS nanoparticle dispersion, and the rest of the dopamine solution (to ensure that the amount of dopamine in the entire system is 20 mg). Put 10mL of the mixed solution in a glass bottle, and place the whole system on a magnetic stirrer to mix and react for 8 hours. During the reaction, it can be observed that the originally clear and transparent reaction system turns into a dark gray opaque solution; , take out the reaction system, centrifuge at 7000rpm, and remove...
Embodiment 3
[0044] Photonic crystal microspheres based on GO and PDA-wrapped silica nanoparticles are used for multiple detection of nucleic acids (the principle of nucleic acid detection is as follows: Figure 5 shown)
[0045] 1. Select unmodified silica nanoparticles (260nm, 230nm, 200nm) of three different particle sizes, and make photonic crystal microspheres (red, green, blue) of three different colors according to the method in example 1.
[0046] 2. Coupling photonic crystal microspheres of three different colors with three different probes (red-probe 1, green-probe 2, blue-probe 3), the coupling method is as follows : Thoroughly wash the photonic crystal microspheres with PBS buffer, and then incubate the microspheres with the ten-fold diluted probe solution for 6 hours. cleaning.
[0047] 3. Divide the probe-coupled photonic crystal microspheres into three groups, and each group contains a certain number of red, green and blue microspheres; in the first group, three kinds of p...
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