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Stem cell gel precursor composition for repairing skin injury and tissue engineering composition using box and application thereof

A technology for repairing skin damage and tissue engineering, which is applied in the field of biomedicine and can solve problems such as stem cells cannot be stably fixed and survived

Inactive Publication Date: 2020-07-03
高尚先
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still many problems in the use of stem cells to treat diabetic foot ulcers. The most important problem is that stem cells cannot be stably fixed and survive on the wound of diabetic foot ulcers. How to stabilize stem cells and survive for a long time through appropriate methods Instead of falling off and inactivating soon, it has become the main bottleneck to solve the problem of stem cells in the local treatment of ulcers (such as diabetic foot ulcers)

Method used

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  • Stem cell gel precursor composition for repairing skin injury and tissue engineering composition using box and application thereof
  • Stem cell gel precursor composition for repairing skin injury and tissue engineering composition using box and application thereof
  • Stem cell gel precursor composition for repairing skin injury and tissue engineering composition using box and application thereof

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Effect test

preparation example Construction

[0063] The embodiment of the present invention also relates to the preparation method of the stem cell gel precursor composition, comprising the following steps:

[0064] When the subcultured stem cells grow to 60%-80%, replace them with serum-free DMEM medium and culture them for 6-12 hours, add trypsin for digestion, then collect the cells, centrifuge and mix with the temperature-sensitive polymer material, The adhesive is mixed with the gel phase transition temperature regulator to obtain the stem cell gel precursor composition.

[0065] The embodiment of the present invention also relates to a tissue engineering composition use kit for repairing skin damage, that is, a kit containing the above-mentioned stem cell gel precursor composition and a film, and the film can fix the gel, moisturize, and isolate the external environment and other effects.

[0066] Specifically, the material of the film can be selected from breathable polymer film. The thickness of the film is pre...

Embodiment

[0071] A stem cell gel precursor composition. The solvent is physiological saline. Under low temperature conditions (1-15° C.), various components are added in proportion. The composition and the mass percentage of each component are shown in Table 1. Wherein, the raw materials used are all commercially available raw materials.

[0072] Table 1

[0073]

Embodiment 9

[0074] Example 9: On the basis of Example 2, a cell-free preparation of Corynebacterium brevis was added, and the added amount was 0.1% of the mass of the stem cell gel precursor composition.

[0075] The effect evaluation method is:

[0076](1) The detection method of phase transition temperature is:

[0077] Take 4mL of the stem cell gel precursor composition solution in a test tube with a thermometer, place the test tube in a constant temperature water bath, the constant temperature water bath starts to heat up slowly from 25°C, reverse the test tube every 15 seconds to observe the gelation situation , when the test tube is reversed but the gel does not flow, record the temperature displayed by the thermometer at this time as the gelation temperature, and measure each sample 3 times in parallel. take the average.

[0078] (2) Adhesion:

[0079] Take commercially available fresh pigskin and wash it in normal saline (37° C.), and use the cleaned skin within 2 hours. Cut a...

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Abstract

The invention relates to the technical field of biological medicine, in particular to a stem cell gel precursor composition for repairing skin injury and a tissue engineering composition using box andapplication thereof. The precursor composition comprises stem cells, a temperature-sensitive polymer material, an adhesive and a gel phase-transition temperature regulator. According to the invention, the temperature-sensitive polymer material is compounded with the stem cells, and the obtained mixture is subjected to phase transformation after being applied to a human body, so that solid gel isformed and adhered to the surface of a wound surface. Meanwhile, the stem cells are stably fixed in the gel. By adding the adhesive, the formed gel can be firmly adhered to the surface of the skin torepair the skin injury.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a stem cell gel precursor composition for repairing skin damage, a tissue engineering composition use box and application thereof. Background technique [0002] Skin damage is a phenomenon of skin damage caused by a variety of diseases or injuries. It is characterized by repeated skin ulceration, loss of partial skin function, and prone to scarring and other skin hyperplasia. Common factors leading to refractory skin damage include skin ulcers, burns, lupus erythematosus, and psoriasis caused by diabetes, microbial inflammation, and immune diseases. [0003] At present, there are more than 425 million diabetic patients in the world. China is the country with the largest number of diabetic patients in the world. Diabetic foot is one of the common serious complications of diabetic patients. In 1999, the World Health Organization defined diabetic foot as diabetes combined with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/18A61L27/20A61L27/38A61L27/50A61L27/52A61L27/54A61L27/60
CPCA61L27/18A61L27/20A61L27/3834A61L27/50A61L27/52A61L27/54A61L27/60A61L2300/406A61L2300/426C08L87/005C08L1/286
Inventor 高尚先
Owner 高尚先
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