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Method for preparing d-lactic acid by fermentation

A lactic acid and lactic acid fermentation technology, applied in the field of microbial fermentation, can solve the problems of equipment, energy consumption and grain consumption, and achieve the effects of short production cycle, high utilization rate, low raw material and production cost

Active Publication Date: 2020-12-25
COFCO NUTRITION & HEALTH RES INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing D-lactic acid by fermentation in order to overcome the problems of high equipment, energy consumption and grain consumption in the D-lactic acid fermentation production process existing in the prior art

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0108] Preparation of saccharification solution: Add 6g of amylase (equivalent to 200U / kg starch dry basis) to 30kg of starch milk with a pH of 5.8 (in which the dry content of starch is 40% by weight), and spray liquefaction with steam at 110°C (the time is about 4s), then flash (pressure difference -0.08±0.02MPa), and keep at 95°C for 60min. Cool down to 60°C by heat exchange to obtain liquefied liquid. Adjust the pH to 5, add glucoamylase, the weight is 0.5‰ of starch dry basis (equivalent to 1000U / kg starch dry basis), stir well, keep at 60°C for 24 hours, and obtain a saccharification solution with a DE value of 99%.

[0109] Tank preparation: add the above-mentioned saccharified liquid directly into the fermenter, and at the same time add sterilized corn steep liquor. The content of carbon element in the liquid is 76g / L). The pH of the fermentation system is controlled by adding 20% ​​of the weight of the fermentation broth to control the pH of the fermentation system ...

Embodiment 2

[0113] Preparation of saccharification solution: Add 6g of amylase (equivalent to 200U / kg starch dry basis) to 30kg of starch milk with a pH of 5.8 (in which the dry content of starch is 40% by weight), and spray liquefaction with steam at 110°C (the time is about 2s), then flash (pressure difference -0.08±0.02MPa), and keep at 95°C for 90min, then cool down to 60°C by heat exchange to obtain liquefied liquid. Adjust the pH to 5, add glucoamylase, the weight is 0.4‰ of starch dry basis (equivalent to 800U / kg starch dry basis), stir evenly, keep at 60°C for 24 hours, and obtain a saccharification solution with a DE value of 96%.

[0114] Tank preparation: add the above-mentioned saccharified liquid directly into the fermenter, and at the same time add sterilized corn steep liquor. The content of carbon element in the liquid is 76g / L). Add 25% milk of lime by weight of the fermentation broth to control the pH to about 6.2±0.2, without monitoring the pH, control the fermentation...

Embodiment 3

[0118] Preparation of saccharification solution: Add 6g of amylase (equivalent to 200U / kg of starch dry basis) to 30kg of starch milk with a pH of 5.8 (wherein the starch dry matter content is 30% by weight), and spray liquefaction with steam at 110°C (the time is about 3s), then flash (pressure difference -0.08±0.02MPa), and keep at 95°C for 60min. Cool down to 60°C by heat exchange to obtain liquefied liquid. Adjust the pH to 5, add glucoamylase, the weight is 0.5‰ of starch dry basis (equivalent to 1000U / kg starch dry basis), stir well, keep at 60°C for 24 hours, and obtain a saccharification solution with a DE value of 99%.

[0119] Tank preparation: add the above saccharification liquid directly into the fermenter, and at the same time add sterilized corn steep liquor, the addition amount makes the nitrogen content in the fermented liquid 3g / L, and controls the glucose content in the fermented liquid to 160g / L (equivalent to So that the content of carbon element in the f...

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Abstract

The invention relates to the field of microbial fermentation, and particularly discloses a method for producing D-lactic acid through fermentation. The method comprises the following step: inoculatinga fermentation culture medium with at least part of a carbon source provided by saccharification liquid against Lactobacillus plantarum with a preservation number of CGMCC No.16835. A production method of the saccharification liquid comprises the steps of mixing a slurry containing a starchy raw material and amylase for spray liquefaction, then conducting flash evaporation, cooling and heat preservation, and then mixing liquefaction liquid and a saccharifying enzyme for saccharification; the dosage of the saccharification liquid makes a content of carbon in the fermentation culture medium equal to 60-80 g / L; and conditions of lactic acid fermentation comprise a temperature of 37-45 DEG C, a pH value of 5.3-6.5, the time of 40-60 h, and the rotating speed of stirring of 50-150 rpm, and theinoculation quantity of the Lactobacillus plantarum makes Lactobacillus plantarum OD600 in a lactic acid fermentation system after inoculation is conducted range from 0.3 to 1. According to the method for producing the D-lactic acid through fermentation, the adopted raw materials have the characters of being wide in source and low in cost, by means of the provided method, production can be conducted in a wide pH value range and a wide temperature range, the workload of raw material pre-treatment is reduced, and the method for producing the D-lactic acid through fermentation has the charactersof being high in sugar utilization rate and conversion rate and short in production cycle.

Description

technical field [0001] The invention relates to the field of microbial fermentation, and specifically discloses a method for preparing D-lactic acid by fermentation. Background technique [0002] D-lactic acid is an important organic acid widely used in food industry, medicine, agriculture, materials and other fields. As a green chemical, it can synthesize polylactic acid, ethyl lactate and other non-toxic and degradable materials, which can be used to manufacture biodegradable plastics, green packaging materials and medical repair materials, etc., to solve the increasingly serious "white pollution" problem, the application prospect is very broad, and it has good social and economic benefits. [0003] The industrial production of D-lactic acid mainly includes chemical synthesis, enzymatic and microbial fermentation. Our country mostly adopts biological fermentation method, using corn, wheat and other starches as raw materials, saccharifying starch through amylase and gluco...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/56C12R1/25
CPCC12P7/56
Inventor 佟毅李义朱威宇周卫强陈博陶进杨凯
Owner COFCO NUTRITION & HEALTH RES INST
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