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Fernleaf hedge bamboo extract, and preparation method and application thereof

A kind of bamboo extract and extract technology, which is applied in the field of Pteris cerevisiae extract and its preparation, can solve the problem of low content of isoorientin and vitexin, difficulty in separation and purification of isoorientin and vitexin, and inconvenience Purification of orientin and vitexin monomers, etc.

Inactive Publication Date: 2020-07-10
JIANGI WENIR NUTRITION HIGH TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are many other carbon glycoside flavonoids in the leaves of Pteris cerevisiae, so it brings difficulties to the separation and purification of isoorientin and vitexin
[0004] In addition, the extraction method of traditional bamboo leaf active ingredients has the problem that the extraction effect is poor. The content of isoorientin and vitexin in the extract of Pteris japonica extracted by traditional method is relatively low, so it is not convenient to extract isoorientin. and purification of vitexin monomer

Method used

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  • Fernleaf hedge bamboo extract, and preparation method and application thereof

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Comparison scheme
Effect test

Embodiment 1

[0031] This embodiment provides a kind of preparation method of Pteris japonica extract, it comprises the following steps:

[0032] S1. First, after drying the bamboo leaves of Pteris cerevisiae, pulverize them and pass through a 40-mesh sieve to obtain the bamboo leaves of Pteris cerevisiae powder; then, after mixing 100 g of bamboo leaves of Pteris phoenix powder with 1 L of 50% ethanol solution, Ultrasonic extraction with a frequency of 40 kHz was carried out for 30 minutes, and suction filtration was performed, and the filter residue was repeatedly extracted once, and the extracts were combined to obtain a crude extract.

[0033] S2. Place the crude extract above at a temperature of 50° C. for vacuum concentration, recover ethanol, and obtain 1 L of concentrated solution.

[0034] S3, the above-mentioned concentrated solution flows through H103 macroporous resin bed (column bed volume is 90mL, column bed height is 50cm) to carry out adsorption operation; Then, carry out el...

Embodiment 2

[0038] This embodiment provides a kind of preparation method of Pteris japonica extract, it comprises the following steps:

[0039] S1. First, after drying the bamboo leaves of Pteris cerevisiae, pulverize them and pass through a 40-mesh sieve to obtain the bamboo leaves of Pteris cerevisiae powder; then, after mixing 100 g of bamboo leaves of Pteris phoenix powder with 1 L of 50% ethanol solution, Ultrasonic extraction with a frequency of 50 kHz was carried out for 30 minutes, and suction filtration was performed, and the filter residue was repeatedly extracted once, and the extracts were combined to obtain a crude extract.

[0040] S2. Place the above crude extract at a temperature of 40° C. for vacuum concentration, recover ethanol, and obtain 1 L of concentrated solution.

[0041] S3, the above-mentioned concentrated solution flows through H103 macroporous resin bed (column bed volume is 90mL, column bed height is 50cm) to carry out adsorption operation; Then, carry out el...

Embodiment 3

[0045] This embodiment provides a kind of preparation method of Pteris japonica extract, it comprises the following steps:

[0046] S1. First, after drying the bamboo leaves of Pteris cerevisiae, pulverize them and pass through a 40-mesh sieve to obtain the bamboo leaves of Pteris cerevisiae powder; then, after mixing 100 g of bamboo leaves of Pteris phoenix powder with 1 L of 50% ethanol solution, Ultrasonic extraction with a frequency of 30 kHz was carried out for 30 minutes, and suction filtration was performed, and the filter residue was repeatedly extracted once, and the extracts were combined to obtain a crude extract.

[0047] S2. Place the above crude extract at a temperature of 60° C. for vacuum concentration, recover ethanol, and obtain 1 L of concentrated solution.

[0048] S3, the above-mentioned concentrated solution flows through H103 macroporous resin bed (column bed volume is 90mL, column bed height is 50cm) to carry out adsorption operation; Then, carry out el...

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Abstract

The invention belongs to the technical field of plant extraction and medicine, and provides a fernleaf hedge bamboo extract, and a preparation method and application thereof. The preparation method ofthe fernleaf hedge bamboo extract comprises the following steps: with fernleaf hedge bamboo leaves as a raw material, extracting the fernleaf hedge bamboo leaves to obtain a fernleaf hedge bamboo leaf extract; adding acid into the fernleaf hedge bamboo leaf extract to enable the final concentration of hydrogen ions in the fernleaf hedge bamboo leaf extract to reach 0.25-2 mol / L, and then carryingout an acid hydrolysis reaction to obtain a hydrolysate; and carrying out extraction separation on the hydrolysate to obtain the fernleaf hedge bamboo extract. According to the method, hydrochloric acid or sulfuric acid is added into the fernleaf hedge bamboo leaf extract for acid hydrolysis reaction, so the content of other bamboo leaf flavonoids and other components in the fernleaf hedge bambooextract can be reduced, the content of isoorientin and vitexin in the fernleaf hedge bamboo extract can be increased, and convenience is brought to purification of isoorientin and vitexin monomers.

Description

technical field [0001] The invention belongs to the technical field of plant extraction and medicine, and in particular relates to an extract of Pteris vulgaris and its preparation method and application. Background technique [0002] Bamboo leaf flavonoids are commonly used food antioxidants and new food resources. Among them, the main functional components in bamboo leaf flavonoids are carboglycoside flavonoids such as isorientin and vitexin. Studies have shown that isorientin and vitexin have anti-oxidation, cardiovascular and cerebrovascular protection, blood sugar lowering, and liver protection. And other pharmacological effects, it is a potential raw material for the development of related drugs. [0003] Fernleaf (Bambusa multiplex cv. Fernleaf) is a kind of bamboo, which is cultivated in southern my country. It has been found through research that the leaves of Pteris phoenix are rich in isoorientin and vitexin, the content of which is higher than that in leaves of...

Claims

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Application Information

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IPC IPC(8): A61K36/899C07D407/04A61K31/352A61P39/06A61P9/00A61P9/10A61P3/10A61P1/16A61K127/00
CPCA61K31/352A61K36/899A61K2236/333A61K2236/51A61K2236/55A61P1/16A61P3/10A61P9/00A61P9/10A61P39/06C07D407/04
Inventor 陈波张清峰曹海燕陈小辉阙素云甘小辉
Owner JIANGI WENIR NUTRITION HIGH TECH
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