Unlock instant, AI-driven research and patent intelligence for your innovation.

Preparation and application of polyvinylamine-arginine graft derivatives as gene carriers

A polyvinylamine and arginine technology, applied in the fields of polymer chemistry and biomaterials, can solve problems such as high toxicity and limited application

Active Publication Date: 2022-07-15
FUJIAN MEDICAL UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this type of polymer is highly toxic to normal cells, which limits its clinical application

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation and application of polyvinylamine-arginine graft derivatives as gene carriers
  • Preparation and application of polyvinylamine-arginine graft derivatives as gene carriers
  • Preparation and application of polyvinylamine-arginine graft derivatives as gene carriers

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0049] Preparation of Polyvinylamine-Arginine Grafted Derivatives.

[0050] The preparation reaction is as follows:

[0051]

[0052] 1) Place N-vinylformamide, 2,2-azodiisobutylamidine dihydrochloride and water in a 100 mL reaction flask, where N-vinylformamide monomer and initiator 2,2- The mass ratio of nitrogen diisobutyl amidine dihydrochloride is 100:1, in the constant temperature water bath at 60~80 ℃, the condensation reflux reaction, the reaction becomes gel-like to the solution, after appropriate cooling, it is used with an appropriate amount of. The water is dissolved and poured out, and the poly-N-vinyl formamide is obtained by precipitation with acetone.

[0053]2) After dissolving the poly-N-vinylformamide obtained in step 1) with water, adding 40wt% NaOH for hydrolysis, then transferring it to a 50 mL reaction flask, and performing reflux hydrolysis at 70-80 °C, and the viscous reaction The solution was poured from the reaction bottle into a clean beaker, t...

example 2

[0072] The complexes of PVAm-Arg and siRNA of Example 1 were transfected in human lung cancer cells.

[0073] A549 human lung cancer cells in logarithmic growth phase were plated at 1.2 × 10 per well. 4 The cell density was seeded in 24-well plates, and then incubated in 5% CO. 2 , and cultured at 37°C for 24 h. When the cell density reaches 70%~80%, the old medium in the culture plate is aspirated, and the PVAm-Arg / siRNA complex nanoparticles obtained in step (3) of Example 1 are added. Each well of the complex contains 0.26 μg of siRNA, with anti-mouse melanoma siRNA as the reporter gene and commercial liposome (GP-siRNA-mateplus) transfection reagent as the positive control. After transfection, washed twice with pre-cooled PBS, solidified by adding 4% glutaraldehyde, then washed three times with PBS for 5 min each time, and finally added DAPI dye at a concentration of 1 μg / mL to each well for nuclei. After staining, the cells were washed with PBS and then transferred to ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses the preparation and application of a polyvinylamine-arginine graft derivative as a gene carrier. First, a two-step synthesis method of free radical polymerization and hydrolysis is used to prepare polyvinylamine, and then a condensing agent is used to dehydrate and condensate polyvinylamine and arginine to prepare polyvinylamine-arginine graft derivatives. The polyvinylamine-arginine gene carrier provided by the invention grafts arginine with a guanidyl structure on the polyvinylamine side chain amino group. The prepared polymer has low toxicity and the particle size of the complex formed with siRNA is small. It has good gene transfection effect in a variety of cancer cells and is a cationic polymer gene carrier with good application prospects.

Description

Technical field [0001] The invention belongs to the technical fields of polymer chemistry and biomaterials, and specifically relates to the preparation of polyvinylamine-arginine graft derivatives and their application in gene carriers. Background technique [0002] Gene therapy is a brand-new disease treatment method developed in the 1990s. Its potential value and effectiveness have opened up a new path for the development of modern medical science. However, current gene vectors have problems such as low transfection efficiency in vivo, high toxicity, and low safety, and cannot meet the needs of clinical applications. [0003] Gene therapy can be divided into two modes: in vitro therapy and in vivo therapy. In vitro treatment refers to obtaining certain cells from the patient's body and genetically modifying them, then proliferating and culturing them, and then transplanting them into the patient's body to achieve the purpose of treatment. This therapy has mature technolo...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C08F126/02C08F8/32C08F8/12C12N15/87
CPCC08F8/32C12N15/87C08F8/12C08F126/02
Inventor 林媚林爱珠刘天惠
Owner FUJIAN MEDICAL UNIV
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com