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A kind of method utilizing microorganism to induce calcium carbonate precipitation

A calcium carbonate and microorganism technology, applied in the field of using microorganisms to induce calcium carbonate precipitation, can solve the problems of slow calcium ion sedimentation rate, reduced reaction rate, waste of calcium resources, etc.

Inactive Publication Date: 2021-05-07
CHINA UNIV OF GEOSCIENCES (BEIJING)
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this process, if the precipitation rate of calcium ions is slow or the amount of precipitation is too small, it will not only reduce the reaction rate, but also waste calcium resources.

Method used

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  • A kind of method utilizing microorganism to induce calcium carbonate precipitation
  • A kind of method utilizing microorganism to induce calcium carbonate precipitation
  • A kind of method utilizing microorganism to induce calcium carbonate precipitation

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Embodiment Construction

[0033] The present invention will be explained in more detail through specific embodiments below, but it is not intended to limit the protection scope of the present invention.

[0034] Bacillus pasteurianus: ATCC11859 (Accession No.), purchased.

[0035] Urea, Calcium Chloride (CaCl 2 ), sodium chloride (NaCl), casein peptone, and soybean peptone were purchased from Shanghai Sinopharm Chemical Reagent Co., Ltd.; 752 UV-Vis Spectrophotometer was purchased from Shanghai Sunny Hengping Scientific Instrument Co., Ltd.

[0036] Sodium montmorillonite was purchased from Zhejiang Fenghong New Material Co., Ltd., and the particle size distribution of the sodium montmorillonite was as follows: figure 1 shown;

[0037] Suspension medium configuration: Add casein peptone (15g / L), soybean peptone (5g / L), NaCl (5g / L) and urea (20g / L) to 1000mL deionized water respectively, adjust the pH value to 7.3, Sterilize at 121°C for 30 minutes.

[0038] Preparation of Bacillus pasteurianus susp...

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Abstract

The invention provides a method for using microorganisms to induce precipitation of calcium carbonate. The method is to mix the bacterial suspension with the calcium reaction solution to obtain the total gel solution, and add sodium montmorillonite to the gel solution to precipitate calcium carbonate; the mass percentage of the sodium montmorillonite in the total gel solution is 0.25%. ~1%. The present invention accelerates the precipitation of calcium ions through the addition of sodium montmorillonite, under the condition of interaction between sodium montmorillonite, reaction solution and urea, and enables calcium ions to achieve complete precipitation, which significantly improves the The efficiency of the reaction has greater practical application value; and under the condition of more reaction liquid, the reaction can be carried out forward, the calcium ions can be completely precipitated, and the precipitation amount of calcium carbonate in the reaction can be obviously increased.

Description

technical field [0001] The invention relates to a method for using microorganisms to induce calcium carbonate precipitation. Background technique [0002] Microbe-induced calcium carbonate precipitation (MICP) is an innovative method for improving soil by urease-catalyzed hydrolysis of urea. With the continuous development of interdisciplinary research in microbiology, geochemistry, civil engineering and other disciplines, microbial-induced calcium carbonate Applied in various fields, this technology has a good effect on improving the strength and stability of soft soil foundations. [0003] At present, the research on MICP mainly focuses on three aspects. One is to focus on improving the strength and durability of cementitious materials through the biomineralization of MICP. MICP gels in situ through calcium-carbon precipitation; one focuses on the precipitation of calcium carbonate minerals induced by MICP in the form of three non-hydratable calcium carbonate crystals: ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00B09C1/10C12R1/07
CPCB09C1/105B09C2101/00C12P3/00
Inventor 唐国旺王贵和贾苍琴王新杰唐俊
Owner CHINA UNIV OF GEOSCIENCES (BEIJING)
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