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In-situ enzymolysis-direct mass spectrometry analysis system and analysis method

A technology of mass spectrometry analysis and in-situ enzymatic hydrolysis, applied in the field of in-situ enzymatic hydrolysis-direct mass spectrometry analysis system, can solve the problems of limited digestion time, affecting the process and efficiency of digestion, limiting the speed of reaction, etc., to achieve intuitive results, The effect of low cost demand

Pending Publication Date: 2020-07-14
SHANDONG ANALYSIS & TEST CENT
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Problems solved by technology

In addition, due to the small volume of the sample, the evaporation of the solvent will seriously affect the digestion process and efficiency, and the digestion time is very limited
In addition, the volume of samples in micro (nano) liters limits the system from stirring and vortexing. Therefore, the inventors found that protein molecules and enzymes can only act through chemical diffusion in the reaction droplets, which greatly limits speed of reaction

Method used

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  • In-situ enzymolysis-direct mass spectrometry analysis system and analysis method
  • In-situ enzymolysis-direct mass spectrometry analysis system and analysis method
  • In-situ enzymolysis-direct mass spectrometry analysis system and analysis method

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Embodiment Construction

[0033] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0034] It should be noted that the following detailed description is exemplary and intended to provide further explanation of the present invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.

[0035] It should be noted that the terminology used here is only for describing specific embodiments, and is not intended to limit exemplary embodiments according to the present invention. As used herein, unless the context clearly dictates otherwise, the singular is intended to include the plural, and it should also be understood that when the terms "comprising" and / or "comprising" are used in this specification, they mean There are features, steps, operations, means, components and / or combinations thereof.

[0036] In...

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Abstract

The invention belongs to the technical field of protein molecule detection, and particularly relates to an in-situ enzymolysis-direct mass spectrometry analysis system and an analysis method. The in-situ enzymolysis-direct mass spectrometry analysis system comprises a micro-enzymolysis pool, a mass spectrometer, an interface and the like, and the enzymolysis pool comprises a columnar part and a capillary part which are communicated with each other; the columnar part is filled with protease and protein sample molecules, and the protease is used for enzymolysis of the protein sample molecules; the inner surface of the columnar part is coated with an adsorption material, and the adsorption material is used for capturing polypeptide fragments subjected to enzymolysis; the tail end of the capillary tube-shaped part is in butt joint with the mass spectrum sample inlet through a spraying structure, and the spraying structure is used for ionizing polypeptide fragments subjected to enzymolysis,so that a mass spectrum analysis device can directly perform mass spectrum analysis. The method can be used for rapid identification of protein molecules in milk and other food and biological tissuemedia.

Description

technical field [0001] The invention belongs to the technical field of protein molecular detection, and in particular relates to an in-situ enzymatic hydrolysis-direct mass spectrometry analysis system and analysis method. Background technique [0002] The statements in this section merely provide background information related to the present invention and do not necessarily constitute prior art. [0003] At present, there are mainly two strategies for protein analysis: "top-down" and "bottom-up". Among them, the most widely used is the "bottom-up" technical route. Through the enzymatic hydrolysis of proteins, protein molecules are digested into polypeptides, and then the peptides are analyzed by mass spectrometry. In order to ensure the sensitivity of the analysis and the mass spectrometry system will not be contaminated by salt or surfactant, the purification steps of protein purification and enzymatic hydrolysis solution system are essential. Typical protein digestion s...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/28G01N27/62
CPCG01N1/28G01N33/6848
Inventor 陈相峰洪翊凌吴日陈德华
Owner SHANDONG ANALYSIS & TEST CENT
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