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Perfusion bioreactor system and perfusion cell culture method

A bioreactor and cell technology, applied in bioreactor/fermenter combination, specific-purpose bioreactor/fermenter, biochemical equipment and methods, etc., can solve problems such as cumbersome use of HyQHarvestainer

Pending Publication Date: 2020-07-14
CORNING INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Understandably, the steps required make using HyQHarvestainer cumbersome

Method used

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  • Perfusion bioreactor system and perfusion cell culture method
  • Perfusion bioreactor system and perfusion cell culture method
  • Perfusion bioreactor system and perfusion cell culture method

Examples

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Embodiment Construction

[0016] Embodiments of the disclosure are described in detail below, examples of which are illustrated in the accompanying drawings. Wherever possible, the same reference numbers will be used throughout the drawings to refer to the same or like parts.

[0017] The singular forms "a", "an" and "the" include plural referents unless the context clearly dictates otherwise. The endpoints of all ranges stating the same feature are independently combinable and inclusive. All references are incorporated herein by reference.

[0018] As used herein, "having", "having", "containing", "including", "comprising", "including" and the like are used in their open sense and generally mean "including but not limited to".

[0019] Unless otherwise specified, all technical and scientific terms used herein have the meaning commonly used in the art. The definitions provided herein are used to help understand certain terms frequently used herein, and do not limit the scope of the present disclosur...

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Abstract

A perfusion bioreactor system is provided herein. The perfusion bioreactor system includes a vessel having a wall at least partially defining an interior compartment for receiving fluid, at least oneport fluidly connected to at least one of a plurality of external sources and at least one outlet fluidly connected to a small pore membrane device.

Description

[0001] Cross References to Related Applications [0002] This application claims the benefit of priority to U.S. Provisional Application Serial No. 62 / 589,669, filed November 22, 2017, the content of which is based upon and incorporated in its entirety herein as if fully set forth below. technical field [0003] The present disclosure relates generally to perfusion bioreactor systems and, more particularly, to perfusion bioreactor systems including components for isolating cells from microcarriers and replacing fluid in the perfusion bioreactor system. Background technique [0004] Microcarrier cell culture is usually performed in bioreactors. During culture, cells grow on the surface of the microcarriers. Once the cell culture process is complete, the cultured cells are detached from the microcarriers, and the cultured solution containing the cells is then separated from the microcarriers for further processing. [0005] One conventional technique for separating the micro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/12C12M1/00C12M1/26
CPCC12M25/14C12M25/16C12M29/10C12M33/14C12M47/02C12M23/28C12M27/02C12M29/18C12M37/00C12M21/08
Inventor R·L·鲁瑟尔
Owner CORNING INC