A kind of Mucor actinosa gd48 and its fermentation product, bacterial agent and application
A technology of Mucor radiata and fermentation products, applied in the field of microbiology, to reduce the occurrence of tomato fusarium wilt
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Embodiment 1A
[0034] Example 1 Separation, purification and identification of Actinomucor elegans GD48
[0035] Healthy tomatoes grown outdoors were collected in Zhaoyuan County, Heilongjiang (45°48'26.47”N 125°09’20.75”E) / min shaker for 30 minutes, and then take out the root to obtain the rhizosphere soil suspension. Take 1ml of the suspension for gradient dilution, and finally take 10 -4 The soil suspension was used for the isolation of fungi.
[0036] The specific operation of the plate dilution coating method is to draw 30 μL of soil suspension on the PDA plate, spread it evenly with a spreader, so that the liquid is completely absorbed by the plate, and repeat the same sample three times. After culturing in a constant temperature incubator at 30°C for 1 week, fungal colonies of different shapes were picked for further purification.
[0037] Perform plate confrontation experiments on different morphological strains for re-screening. The operation method is as follows:
[0038] A 4m...
Embodiment 2
[0045] The control effect of embodiment 2 tomato sickle wilt
[0046] In this example, a pot experiment was used to verify the antagonistic effect of the strain Actinomucor elegans GD48 on fusarium wilt of tomato in the greenhouse.
[0047] In this example, the GD48 spore suspension was first prepared, that is, the preserved strain was first inoculated on a solid PDA medium, and after the mycelium was overgrown with the medium, a small amount of mycelia was picked in a conical flask containing a liquid PDB medium, and placed at 37°C. , 150r / min shaking culture for 12-16h, remove the mycelia from the obtained culture solution by filtering to obtain a spore suspension, and then count with a hemocytometer under a microscope to count the concentration of the spore suspension. The spore suspension counting method is to use a hemocytometer under a microscope to first measure the number of spores in each small square, and then convert it into the number of spores per milliliter of ba...
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