Immobilized enzyme used for nitrogen removal of sewage and preparation method and application thereof

A technology of immobilized enzymes and denitrification, applied in biochemical equipment and methods, chemical instruments and methods, immobilized on/in organic carriers, etc., can solve the problems of low wastewater treatment efficiency and long water treatment time, etc. Reach the effect of high removal efficiency of ammonia nitrogen

Inactive Publication Date: 2020-07-28
北京博泰至淳生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the process of wastewater treatment using the above method, although the removal efficiency of nitrate nitrogen or ammonia nitrogen is relatively high in the wastewater treatment process, there is also the problem of long water treatment time. Generally, the qualified time for wastewater treatment for more than 3 days
This results in less efficient wastewater treatment

Method used

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  • Immobilized enzyme used for nitrogen removal of sewage and preparation method and application thereof
  • Immobilized enzyme used for nitrogen removal of sewage and preparation method and application thereof
  • Immobilized enzyme used for nitrogen removal of sewage and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A preparation method for immobilized enzymes for sewage denitrification, said method comprising the following steps:

[0039] S1 prepares nitrifying bacteria and denitrifying bacteria cells for use, specifically including the following steps:

[0040] S1-1 Take the return sludge from the secondary sedimentation tank of the sewage plant, filter it through a 30-mesh nylon net to remove impurities such as larger particles, then wash and centrifuge it with normal saline for 2-3 times, and inoculate the concentrated activated sludge separately Carry out strain culture in the culture medium of nitrifying bacteria and denitrifying bacteria;

[0041] The sample inoculated into the nitrifying bacteria medium was cultured for 30 days at a temperature of 30°C and a pH of 7.5; the sample inoculated into a denitrifying bacteria medium was cultured at a temperature of 30°C, dissolved oxygen of 0.3mg / L, and a pH of 7.0 10 days of cultivation time under the condition;

[0042] S1-2 C...

Embodiment 2

[0049] A preparation method for immobilized enzymes for sewage denitrification, said method comprising the following steps:

[0050] S1 prepares nitrifying bacteria and denitrifying bacteria cells for use, specifically including the following steps:

[0051] S1-1 Take the return sludge from the secondary sedimentation tank of the sewage plant, filter it through a 30-mesh nylon net to remove impurities such as larger particles, then wash and centrifuge it with normal saline for 2-3 times, and inoculate the concentrated activated sludge separately Carry out strain culture in the culture medium of nitrifying bacteria and denitrifying bacteria;

[0052] The sample inoculated into the nitrifying bacteria medium was cultured for 25 days at a temperature of 25°C and a pH of 7.0; the sample inoculated into a denitrifying bacteria medium was cultured at a temperature of 25°C, dissolved oxygen of 0.3mg / L, and a pH of 6.5 The culture time under the condition of 7 days;

[0053] S1-2 Ce...

Embodiment 3

[0060] A preparation method for immobilized enzymes for sewage denitrification, said method comprising the following steps:

[0061] S1 prepares nitrifying bacteria and denitrifying bacteria cells for use, specifically including the following steps:

[0062] S1-1 Take the return sludge from the secondary sedimentation tank of the sewage plant, filter it through a 30-mesh nylon net to remove impurities such as larger particles, then wash and centrifuge it with normal saline for 2-3 times, and inoculate the concentrated activated sludge separately Carry out strain culture in the culture medium of nitrifying bacteria and denitrifying bacteria;

[0063] The sample inoculated into the nitrifying bacteria medium was cultured for 35 days at a temperature of 35°C and a pH of 8.0; the sample inoculated into a denitrifying bacteria medium was cultured at a temperature of 35°C, dissolved oxygen of 0.48mg / L, and a pH of 7.5 15 days of cultivation time under the condition;

[0064] S1-2 ...

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PUM

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Abstract

The invention discloses an immobilized enzyme for nitrogen removal of sewage and a preparation method and application thereof, and relates to the technical field of sewage treatment. The preparation method for the immobilized enzyme comprises the following steps: S1, preparing nitrifying bacteria and denitrifying bacteria for later use; S2, performing ultrasonication and then centrifugation on thebacteria to obtain a crude enzyme extract; S3, adding active carbon to the crude enzyme solution for mixed adsorption; and S4, performing enzyme immobilization: adding a carrier solution containing polyvinyl alcohol and sodium alginate to the concentrated enzyme solution, then, dropwise adding the mixed solution into a saturated boric acid solution containing 2% anhydrous CaCl2 to obtain immobilized beads after standing, and performing washing with deionized water to prepare the immobilized enzyme for nitrogen removal. The immobilized enzyme can be used for removing ammonia nitrogen in the sewage, and has the advantages of a simple preparation method, reusability, low production cost, and the like.

Description

technical field [0001] The invention relates to the technical field of sewage treatment, more specifically, it relates to an immobilized enzyme for denitrification of sewage and its preparation method and application. Background technique [0002] At present, one of the main factors of surface water pollution is that the total nitrogen content in the water body is too high (total nitrogen includes ammonia nitrogen and nitrate nitrogen), which leads to eutrophication of the water body, and the growth of green algae in the water body increases. The amount of dissolved oxygen in water is small, which is not conducive to the growth of aquatic animals and plants. Secondly, the ammoniacal nitrogen (NH 3 ) is too high not only to prevent the ammonia in the organism from being discharged from the body, but also to penetrate into the body from the water, reduce or stagnate the metabolism of aquatic organisms, damage some important organs including gills, inhibit their growth and dev...

Claims

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Application Information

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IPC IPC(8): C12N11/10C12N9/00C12N1/20C12N1/02C02F3/34
CPCC02F3/342C12N1/02C12N1/20C12N9/00C12N11/10
Inventor 朱琳琳汪翠萍郑淑文张阳阳秦梓耕王战台潘建通陈凯华迟金宝
Owner 北京博泰至淳生物科技有限公司
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