Method for identifying production place of pericarpium citri reticulatae based on autofluorescence-quenched fluorescence spliced spectrum

An autofluorescence and fluorescence spectrum technology, which is applied in the field of identifying the origin of tangerine peel based on autofluorescence-quenching fluorescence splicing spectrum, can solve the problems of requiring large analytical instruments, consumption, and many harmful organic reagents, and achieves high sensitivity, strong specificity, The effect of improving the recognition rate

Active Publication Date: 2020-07-28
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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Problems solved by technology

Although these methods can accurately distinguish tangerine peel from other origins, they all have the disadvantages of long sample testing time, large and expensive analytical instruments, and more harmful organic reagents.

Method used

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  • Method for identifying production place of pericarpium citri reticulatae based on autofluorescence-quenched fluorescence spliced spectrum
  • Method for identifying production place of pericarpium citri reticulatae based on autofluorescence-quenched fluorescence spliced spectrum
  • Method for identifying production place of pericarpium citri reticulatae based on autofluorescence-quenched fluorescence spliced spectrum

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Embodiment 1

[0049] A method for identifying the origin of tangerine peel based on autofluorescence-quenched fluorescence mosaic spectrum, comprising the steps of:

[0050] 1) Synthesis of CdTe quantum dots:

[0051] Add 0.0457g of cadmium dichloride and 18 μL of mercaptoacetic acid into 50mL of ultrapure water, stir at normal temperature and pressure to fully dissolve it; then add 1.0mol / L of sodium hydroxide solution to adjust the pH value of the resulting solution to 10.3, and then Introduce nitrogen and stir for 20 minutes under ice bath conditions; add 0.0089g sodium tellurite, stir for 15 minutes, then add 0.08g sodium borohydride, continue stirring for 10min; transfer the resulting clear solution to an oil bath, 100 ℃ oil bath refluxed for 1.5h, and the emission wavelength was about 550nm, and the orange-yellow CdTe quantum dot fluorescent probe (its fluorescence spectrum as shown in Fig. figure 2 shown);

[0052] 2) Preparation of dried tangerine peel aqueous extract:

[0053] ...

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Abstract

The invention discloses a method for identifying the production place of pericarpium citri reticulatae based on an autofluorescence-quenched fluorescence spliced spectrum. The method comprises the following steps: measuring a fluorescence spectrum of a pericarpium citri reticulatae sample and a fluorescence quenching spectrum of the pericarpium citri reticulatae sample added with CdTe quantum dots; converting and splicing the obtained spectral data to obtain an autofluorescence-quenched fluorescence spliced spectrum; and establishing an intelligent discrimination model according to the characteristic difference information integrated by the spliced spectrum and the correlation characteristics of different pericarpium citri reticulatae production places, so as to realize effective identification of unknown pericarpium citri reticulatae production places. Compared with the traditional method for identifying the production place of pericarpium citri reticulatae by chromatography and the like, the method has the advantages of low instrument cost, high detection speed, high sensitivity, strong production place identification capability and the like, and has practical application value in the field of traditional Chinese medicine quality control.

Description

technical field [0001] The invention belongs to the technical field of quality control of traditional Chinese medicines, and in particular relates to a method for identifying the origin of tangerine peel based on autofluorescence-quenched fluorescence splicing spectrum. Background technique [0002] Tangerine peel is the dry and mature peel of Citrus Reticulata Blanco and its cultivars of the Rutaceae plant. It has the functions of regulating qi and invigorating the spleen, drying dampness and resolving phlegm. Chenpi medicinal materials are divided into "Guangchenpi" and "Chenpi" due to different cultivars and places of origin. The Chinese Pharmacopoeia (2015 edition) indicates that the source of tangerine peel is four orange cultivars, including Chazhimandarin (Guangdong), Dahongpao (Sichuan), Wenzhou Satsuma (Zhejiang) and Fuju (Fujian). Among them, "Guang Chenpi" is the dry and mature peel of C.Reticulata'Chachi' which has been stored for more than three years. It is of...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/6428G01N2021/0131G01N2021/0143G01N2021/6417G01N2021/6432
Inventor 付海燕兰薇王硕陈亨业时琼
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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