High-throughput method for detecting tooth stain prevention and removal

一种牙渍、污渍的技术，应用在高效测定法领域

Active Publication Date: 2020-07-28
JOHNSON & JOHNSON CONSUMER COPANIES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The above method is labor intensive and therefore not an efficient method for screening test formulations for stain prevention or removal efficacy

Method used

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  • High-throughput method for detecting tooth stain prevention and removal
  • High-throughput method for detecting tooth stain prevention and removal

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: decontamination effect test

[0034] The following general process is used and described in more detail below:

[0035] Decontamination effect test process :

[0036] 1. Formation of a surface coat on hydroxyapatite (HAp) particles by mixing the particles with saliva and incubating the mixture.

[0037] 2. Transfer the HAp particle / saliva suspension to the bulk filter vessel.

[0038] 3. The saliva supernatant was filtered and discarded.

[0039] 4. Obtain stain particles by adding the stain solution directly to the filter vessel and incubating.

[0040] 5. Transfer the membrane-coated fouling HAp particle suspension to the filter vessel.

[0041] 6. Discard the supernatant by filtering out the membrane-coated contaminating HAp particles.

[0042] 7. Resuspend the membrane-coated fouling HAp particles in water and optionally store for future testing.

[0043] 8. Preparation of membrane-coated fouling HAp particles for processing.

[0044] 9. Dis...

Embodiment 2

[0049] Embodiment 2: antifouling test

[0050] The following general process is used and described in more detail below:

[0051] Anti-fouling test process :

[0052] 1. Formation of a surface coat on hydroxyapatite (HAp) particles by mixing the particles with saliva and incubating the mixture.

[0053] 2. Transfer the HAp particle / saliva suspension to the bulk filter vessel.

[0054] 3. The saliva supernatant was filtered and discarded.

[0055] 4. Discard the supernatant by processing the membrane-coated HAp particles.

[0056] 5. Discard the supernatant by filtering the treated membrane-coated HAp particles.

[0057] 6. Stain the membrane-coated pretreated HAp particles by adding the stain solution directly to the filter vessel and incubating.

[0058] 7. Wash any loosely attached stains off the pellicle-coated pretreated HAp particles.

[0059] 8. Use 5% PPi solution to elute the stain on the membrane-coated pre-stained HAp particles.

[0060] 9. Filter and coll...

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PUM

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Abstract

Provided are methods of detecting tooth stain prevention and / or removal comprising the steps of providing pellicle-coated hydroxyapatite particles, contacting the particles with a first solution and then a second solution, wherein the first solution is either a test solution or a stain solution and the second solution is the other of said test solution or stain solution, separating from the resulting particles a liquid comprising stain introduced in the contacting step b), and measuring the UV-Vis absorbance of the separated liquid to determine the amount of stain in the liquid.

Description

technical field [0001] The present invention relates to a highly efficient assay for the identification of extrinsic stain prevention and / or removal molecules. Background technique [0002] There are many reasons for tooth staining. These include the regular consumption of products containing polluting chemicals or colored substances, such as food, beverages (coffee, tea, cola, wine), or smoking tobacco. In other cases, medications, disease, and poor oral hygiene can lead to tooth staining. Staining affects tooth aesthetics and can also be a contributing factor to caries and loss. [0003] A number of product formulations have been developed to prevent or remove stains on teeth. These include bleaches or oxidizing agents, or formulations that do not contain oxidizing agents. In the development of these formulations, a significance test is required to show formulation efficacy. [0004] Some of the efficacy testing procedures developed use bovine or human teeth, or ceram...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61Q11/00A61K8/24A61K8/02G01N1/30G01N21/17G01N33/15
CPCA61K8/24A61K8/0241G01N21/17A61K2800/62A61Q11/00A61K6/52A61K6/17G01N1/30A61L27/12
Inventor M·C·吉亚诺D·奎罗兹S·诺克司
Owner JOHNSON & JOHNSON CONSUMER COPANIES
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