Microscopic electrical impedance imaging device and method based on diamond nv color center

Abstract

A microscopic electrical impedance imaging device based on diamond NV color centers, including: a magnetic field imaging module, using diamond ensemble NV as a weak magnetic field detector; a microwave radiation structure module, using an Ω-shaped radiation structure as a microwave antenna to send diamond NV color centers The microwave magnetic field is radiated; the microelectrode module uses a cross-shaped iridium-iridium oxide metal electrode with two pairs of electrode arms to inject an alternating current into the buffer solution. The present invention expands the spatial resolution of the existing magnetic resonance electrical impedance imaging, and improves the spatial resolution of the electrical impedance imaging to the micron to submicron scale, thereby realizing a microscopic electrical impedance imaging method, which is suitable for cells Conductivity imaging of biological samples.

Description

technical field [0001] The invention relates to the technical field of biological cell parameter measurement, in particular to a microscopic electrical impedance imaging device and method based on diamond NV color centers. Background technique [0002] The cell membrane does not conduct electricity, and the cytoplasm and the liquid environment of the cell contain electrolyte solution, which is conductive. Physiological and pathological states of cells will change the permeability of the cell membrane, causing changes in the electrical impedance of the cell (which degenerates into conductivity at low frequency approximations) and its distribution. The electrical impedance of cells or biological tissues reflects the physiological state of cells, and the electrical impedance imaging of biological tissues has important application value in medical diagnosis and biomedical research. Especially in the diagnosis of cancer, imaging the conductivity of biological samples can provide...

AI Technical Summary

Problems solved by technology

[0007] The disadvantage is that the spatial resolution of this conductivity imaging method is usually a few millimeters, which is suitable for conductivity imaging at the macroscopic scale of tissues and organs, but is no longer suitable for microscopic conductivity imaging at the cellular or subcellular level.

This limits the further application of conductivity imaging in disease diagnosis and biomedical research

In addition, according to the actual measurement results in the literature, the image reconstructed by this algorithm has large errors, and blurring and artifacts are prone to appear at the jumps in the conductivity distribution, and the accuracy and stability of the solving algorithm are insufficient.

Since the finite element grid calculation technology used in this algorithm needs to manually adjust the grid boundaries, there is randomness in the boundary setting, and the repeatability of the results is insufficient.

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