Method for determining content of six polyphenol compounds in compound red skin blood-enriching oral liquid

A technology of polyphenolic compounds and determination methods, which is applied in the field of content determination of six polyphenolic compounds in compound Hongyi Buxue Oral Liquid, can solve the problems of lack of content determination of polyphenolic components, and achieve simple and repeatable methods Good results

Inactive Publication Date: 2020-08-07
白青山
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, the quality research of peanut red coat is evaluated by the content of single components of catechin and resveratrol, and the content determination of other polyphenolic components is lacking.

Method used

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  • Method for determining content of six polyphenol compounds in compound red skin blood-enriching oral liquid
  • Method for determining content of six polyphenol compounds in compound red skin blood-enriching oral liquid
  • Method for determining content of six polyphenol compounds in compound red skin blood-enriching oral liquid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Methodological Experiment of the Content Determination Method of 6 Kinds of Polyphenol Compounds in Compound Hongyi Buxue Oral Liquid

[0032] 1 material

[0033] 1.1 Instrument

[0034] 1290 type ultra-high performance liquid chromatography, including online vacuum degassing device, quaternary gradient pump, automatic sampler, column thermostat and DAD detector (Agilent, USA); Mettler-Toledo XP 205 electronic analytical balance (Mettler- Toledo Corporation).

[0035] 1.2 Drugs and reagents

[0036]Caffeic acid reference substance (batch number: 110885-201703, purity: 99.7%), gallic acid reference substance (batch number: 110831-201605, purity: 90.8%), ferulic acid reference substance (batch number: 110773-201614, purity: 99.0%) ), catechin reference substance (batch number: 110877-201604, purity: 99.2%), and epicatechin reference substance (batch number: 110878-201703, purity: 99.7%) were all purchased from China National Institutes for Food and Drug Contr...

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Abstract

The invention belongs to the technical field of chemical detection, and particularly relates to a method for determining the content of six polyphenol compounds in a compound red skin blood enrichingoral liquid. The polyphenol compounds are caffeic acid, gallic acid, p-hydroxycinnamic acid, ferulic acid, catechinic acid and epicatechin, and the content determination method is a UPLC method. Methodology experiment results show that the linear ranges of the caffeic acid, gallic acid, p-hydroxycinnamic acid, ferulic acid, catechinic acid and epicatechin are respectively 2.35 to 235.00 mu g/mL, 0.20 to 20.00 mu g/mL, 0.27 to 27.00 mu g/mL, 0.63 to 63.00 mu g/mL, 1.80 to 180.00 mu g/mL and 0.26 to 260.00 mu g/Ml, the limits of quantitation are 105 ng/mL, 100 ng/mL, 135 ng/mL, 315 ng/mL, 90 ng/mL and 130 ng/mL respectively, and the limits of detection are 36 ng/mL, 33 ng/mL, 45 ng/mL, 105 ng/mL, 30 ng/mL and 43 ng/mL respectively; the RSD of precision, stability and repeatability tests is less than 2.0%; and the average sample adding recovery rate is 98.07% respectively.

Description

technical field [0001] The invention belongs to the technical field of chemical detection, and in particular relates to a method for determining the content of six polyphenolic compounds in Compound Hongyi Buxue Oral Liquid. Background technique [0002] Compound Hongyi Buxue Oral Liquid is refined from peanut Hongyi, fungus, jujube and wolfberry as raw materials. It has the effects of nourishing blood, nourishing qi and invigorating spleen, and is used as an adjuvant treatment for iron deficiency anemia. Among them, peanut red coat is its main component. Peanut red coat, also known as peanut skin, long fruit coat, and peanut coat, is the seed coat of Arachis hypogaea Linn., a plant of the legume family Arachis hypogaeaLinn. . Polyphenols are a general term for a class of compounds with polyphenolic hydroxyl groups, which have various physiological and pharmacological activities such as antibacterial, anti-inflammatory, anti-oxidation, radiation protection, and blood press...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8675G01N2030/047
Inventor 白青山
Owner 白青山
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