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A kind of bio-organic fertilizer containing methylotrophic bacillus and its preparation

A methyl nutrient type, bio-organic fertilizer technology, applied in the directions of organic fertilizers, microorganism-based methods, biochemical equipment and methods, etc., can solve problems such as the inability of bacillus inoculants to be stored, and achieve the improvement of bacterial growth ability, The effect of improving soil physical and chemical properties and soil microecology

Active Publication Date: 2022-02-08
SHANDONG AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Aiming at the current shortage of tomato fusarium wilt bio-organic fertilizers and the lack of storage resistance of bacillus bacteria, the present invention provides a bio-organic fertilizer containing methylotrophic bacillus, which can not only reduce the ammonium nitrogen content in the soil, but also increase the nitrate nitrogen content. Nitrogen content, also able to promote an increase in the number of bacteria and Bacillus in the tomato rhizosphere soil and reduce the number of fungi and Fusarium oxysporum

Method used

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  • A kind of bio-organic fertilizer containing methylotrophic bacillus and its preparation
  • A kind of bio-organic fertilizer containing methylotrophic bacillus and its preparation
  • A kind of bio-organic fertilizer containing methylotrophic bacillus and its preparation

Examples

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Effect test

Embodiment 1

[0030] Example 1 Toxicity of Methylotrophic Bacillus TA-1 to Tomato Fusarium Wilt

[0031] The methylotrophic Bacillus TA-1 was first disclosed in the Chinese invention patent application CN 108641989 A, the publication date was 2018.10.12.

[0032] 1. Antagonistic activity of TA-1 cells against Fusarium wilt of tomato

[0033] Determination of the effect of TA-1 bacteria on tomato Fusarium wilt ( Fusarium oxysporum f. sp. Lycopersici ) antibacterial activity. Under sterile conditions, activate Fusarium wilt of tomato, use a puncher with a diameter of 5 mm to take a cake at the edge of the colony, inoculate it in the center of a PDA plate (9 cm), dip a toothpick into TA-1 of a single colony, And place it at a distance of 2.5 cm from the bacteria cake of pathogenic bacteria. Take the PDA plate only inoculated with pathogenic bacteria as a control, and culture it at a constant temperature of 28°C. When the mycelium of the pathogenic bacteria in the control group covers the ...

Embodiment 2

[0038] Example 2 The screening of methylotrophic bacillus TA-1 fermentation conditions

[0039] 2.1 Determination of TA-1 growth curve and determination of inoculation age

[0040] A single colony of TA-1 was picked and put into LB liquid medium with a volume of 100 mL / 250 mL, cultured at 30°C and 180 rpm, and the fermentation broth of different periods was collected every 2 h. Using the uninoculated LB liquid medium as a control, measure the OD of the fermentation broth with a spectrophotometer 600 . Taking the culture time as the abscissa and the OD value of the fermentation broth as the ordinate, draw the growth curve of TA-1 to determine the inoculation age of TA-1 fermentation.

[0041] The growth curve of strain TA-1 is as follows Figure 4 shown. From 0 to 6 hours of culture, the OD value rose slowly and was at a low level, indicating that the strain was in the growth delay phase, and the strain reproduced slowly; from 6 h to 12 h, the OD value increased sharply, in...

Embodiment 3

[0060] Example 3 Preparation of Methylotrophic Bacillus TA-1 Biological Organic Fertilizer

[0061] TA-1 bio-organic fertilizer: TA-1 is obtained according to the optimized medium and optimized culture conditions in Example 2 to obtain a fermentation broth containing TA-1 bacteria, and the amount of viable bacteria is adjusted to be no less than 3×10 8 1 L of CFU / mL fermentation broth; add 700 g of humic acid adsorption fermentation broth, then add 300 g of yellow clay, mix evenly, granulate, dry at 50°C, repeat the preparation of 5 batches, and determine the number of viable bacteria by plate colony counting method: In each batch of bio-organic fertilizer, the number of viable bacteria in TA-1 was 3×10 8 CFU / g or more.

[0062] According to the same formula and ratio, the granules of the control organic fertilizer were prepared with sterile water instead of the fermented liquid.

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Abstract

The invention provides a bio-organic fertilizer containing methylotrophic bacillus, comprising methylotrophic bacillus TA-1; the number of effective viable bacteria is not less than 3×10 8 CFU / g. Among the organic fertilizers provided by the present invention, TA-1 has a higher antagonistic activity to tomato wilt indoors, and has the potential to prepare bio-organic fertilizers; the liquid fermentation conditions of TA-1 have been obtained by single factor and response surface method, and in the optimization Under the conditions of greenhouse conditions, it can not only improve the growth ability of the bacteria, but also stimulate the production of more antibacterial secondary metabolites; the bio-organic fertilizer made from TA‑1 fermentation broth can improve the physical and chemical properties of soil and increase the yield of tomato under greenhouse conditions. It can also effectively control tomato wilt and regulate soil microbial flora to improve soil and prevent diseases for a long time.

Description

technical field [0001] The invention belongs to the field of microbial application technology and biological control, and in particular relates to a methylotrophic bacillus bio-organic fertilizer and its preparation. Background technique [0002] my country is the country with the largest tomato planting area and the largest output in the world. In recent years, soil-borne diseases have become more and more serious, and have become a bottleneck limiting the sustainable production of tomato. Among them, Fusarium wilt of tomato (FOL) is caused by Fusarium oxysporum tomato specialization ( Fusarium oxysporum f. sp. Lycopersici ) is a soil-borne disease that seriously affects tomato yield and quality. [0003] At present, the control of tomato soil-borne diseases in my country is still dominated by chemical agents. The frequent and irrational use of fungicides not only easily leads to the outbreak of pathogenic bacteria resistance, but also poses a serious threat to the ecol...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C05F11/08C05G3/00C05G3/60C05G3/80C05G5/12C12N1/20A01G22/05A01N63/22A01P3/00C12R1/07
CPCC05D9/00C05G3/00C05G3/60C05G3/80C05G5/12C12N1/20A01G22/05A01N63/22C05F11/02C05F11/08
Inventor 乔康孟臻姬小雪丁新华
Owner SHANDONG AGRICULTURAL UNIVERSITY
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