Application of PTPMT1 as lung cancer diagnosis marker and/or treatment target

A technology for the diagnosis and treatment of lung cancer, which can be used in medical preparations containing active ingredients, drug combinations, and biomaterial analysis.

Active Publication Date: 2020-08-11
青海省人民医院 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The biological characteristics of SCLC are poor differentiation, rapid proliferation, and high invasiveness of cancer cells, so it is found clinically that it is prone to early metastasis, and the 5-year survival rate is less than 10%. Although it is highly sensitive to radiotherapy and chemotherapy, most patients have remission after first-line treatment After a short period of time, the disease relapses, and drug resistance is prone to appear after relapse
At present, there are not many studies on the regulation and function of PTPMT1 expression

Method used

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  • Application of PTPMT1 as lung cancer diagnosis marker and/or treatment target
  • Application of PTPMT1 as lung cancer diagnosis marker and/or treatment target
  • Application of PTPMT1 as lung cancer diagnosis marker and/or treatment target

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Experimental program
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Effect test

Embodiment 1

[0028] 1. Experimental method

[0029] 1.1. Verify the expression of interference PTPMT1 in A549 cells

[0030] (1) A549 cells by 2×10 5 Spread the cells in a six-well plate, and add interference PLKO.1-PTPMT-shRNA, PLKO.1-Control recombinant lentivirus, high-expression PTPMT and control recombinant lentivirus according to 10MOI after the cells adhere to the wall, and then add 4μl polybrene / well , continue culturing after cross-mixing;

[0031] (2) GFP green fluorescence was observed under a fluorescent microscope after virus infection for 48 hours;

[0032] (3) trypsinize the cells, centrifuge and collect;

[0033] (4) Flow cytometric detection of A549 infection interference PLKO.1-PTPMT, PLKO.1-Control recombinant lentivirus and high-expression PTPMT and control recombinant lentivirus cells GFP green fluorescence expression efficiency.

[0034] 1.2.Q-PCR method to verify the expression of interference ShPTPMT and highly expressed PTPMT in A549 cells:

[0035] (1) A549 c...

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Abstract

The invention provides application of PTPMT1 as a lung cancer diagnosis marker and / or a treatment target, and belongs to the technical field of tumor diagnosis and treatment. By inhibiting PTPMT1, theproliferation activity of A549 cells can be obviously reduced. PTPMT1 is an important energy regulation and control molecule for maintaining lung cancer cell proliferation and survival. The inhibition of PTPMT1 can obviously reduce the glucose metabolism of A549 cells. PTPMT1 participates in glycolysis and regulation of mitochondria in an anoxic reaction. PTPMT1 affects the mechanism of cell glycometabolism and may secrete insulin, promote the utilization of sugar, regulate Sirt1 and other mechanisms. After the PTPMT1 is knocked out, the function of influencing cell mitochondria is possibly related to the reduction of the membrane function of the mitochondria. It is indicated that PTPMT1 is a diagnosis molecule and a possible treatment target of lung cancer.

Description

technical field [0001] The invention belongs to the technical field of tumor diagnosis and treatment, and in particular relates to the application of PTPMT1 as a diagnostic marker and / or therapeutic target for lung cancer. Background technique [0002] Worldwide, lung cancer ranks first among cancer-related deaths among men and ranks second after breast cancer among women. Lung cancer includes non-small cell lung cancer (NSCLC) and small cell lung cancer (SCLC), of which the latter accounts for about 20%. SCLC is characterized by poor differentiation, rapid proliferation and high invasiveness of cancer cells, so it is found clinically that it is prone to early metastasis , the 5-year survival rate is less than 10%. Although it is highly sensitive to radiotherapy and chemotherapy, most patients will relapse within a short time after remission of first-line treatment, and drug resistance is prone to appear after relapse. Because traditional radiotherapy and chemotherapy do not...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573G01N33/574A61K45/00A61P11/00A61P35/00
CPCG01N33/573G01N33/57423G01N33/57496A61K45/00A61P35/00A61P11/00G01N2333/916
Inventor 徐芹芹王立生高川成阚捷王新国赞梅
Owner 青海省人民医院
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