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Application of anti-inflammatory and analgesic bolus and preparation thereof in preparation of medicine for promoting refractory wound healing

A technique for wound healing and Pien Tze Huang, which is applied in the field of biomedicine to achieve the effects of promoting secretion, wound healing and proliferation.

Inactive Publication Date: 2020-08-25
ZHANGZHOU PIEN TZE HUANG PHARM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The application of Pien Tze Huang and its preparations in the preparation of drugs for promoting the healing of refractory wounds has not been reported

Method used

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  • Application of anti-inflammatory and analgesic bolus and preparation thereof in preparation of medicine for promoting refractory wound healing
  • Application of anti-inflammatory and analgesic bolus and preparation thereof in preparation of medicine for promoting refractory wound healing
  • Application of anti-inflammatory and analgesic bolus and preparation thereof in preparation of medicine for promoting refractory wound healing

Examples

Experimental program
Comparison scheme
Effect test

experiment example 1

[0044] Experimental Example 1 Animal Pharmacodynamics Experiment

[0045] 1. Drugs and reagents

[0046] Preparation of sodium citrate buffer solution: add 2.10g of citric acid to 100ml of double distilled water to make citric acid mother liquor, which is called liquid A; add 2.94g of trisodium citrate to 100ml of double distilled water to make sodium citrate mother liquor, called Solution B: Mix A and B solutions at a ratio of 1:1.32, measure the pH value with a pH meter, adjust the pH of the solution to 4.0 with citric acid, and obtain sodium citrate buffer.

[0047] Preparation of streptozotocin (STZ) solution: Dissolve streptozotocin in the above-mentioned sodium citrate buffer, freshly prepare STZ solution with a concentration of 10 mg / mL, and sterilize by filtration with a 0.22 μm bacterial filter. Pay attention to the preparation in the dark, and use it now.

[0048] The preparation of Pien Tze Huang gel: the Pien Tze Huang of 1 weight part is mixed with the poloxamer...

experiment example 2

[0056] Experimental Example 2 Immunohistology Experiment

[0057] 1. Drugs and reagents

[0058] Sodium citrate buffer preparation: add 2.10g of citric acid to 100ml of double distilled water to make citric acid mother solution, called A solution; add 2.94g of trisodium citrate to 100ml of double distilled water to make sodium citrate mother solution, called B solution; mix A and B solutions at a ratio of 1:1.32, measure the pH value with a pH meter, and adjust the pH of the solution to 4.0 with citric acid to obtain sodium citrate buffer.

[0059] Preparation of streptozotocin (STZ) solution: Dissolve streptozotocin in the above-mentioned sodium citrate buffer, freshly prepare STZ solution with a concentration of 10 mg / mL, and sterilize by filtration with a 0.22 μm bacterial filter. Pay attention to the preparation in the dark, and use it now.

[0060] The preparation of Pien Tze Huang gel: the Pien Tze Huang of 1 weight part is mixed with the poloxamer of 10 weight parts, ...

experiment example 3

[0079] Experimental Example 3 Determination of the total antioxidant capacity of Pien Tze Huang by ABTS method

[0080] 1. Experimental method

[0081] Using the total antioxidant capacity test kit (purchased from Biyuntian Biotechnology Co., Ltd.), the test was carried out according to the method shown in the kit manual, and an appropriate amount of ABTS (2,2-azino-di( 3-ethyl-benzothiazole-6-sulfonic acid) diammonium salt) working solution, first prepare the ABTS working mother solution with 100 μL ABTS solution and 100 μL oxidant solution. After the ABTS working mother solution is prepared, it can be used after being stored at room temperature in the dark for 15 hours. When used, the ABTS working stock solution was diluted 50 times with PBS. 10mM Trolox (water-soluble vitamin E) standard solution was diluted into 0.15, 0.3, 0.6, 0.9, 1.2 and 1.5mM working solution with PBS solution. Add 200 μL ABTS working solution to each detection well of the 96-well plate, add 10 μL d...

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Abstract

The invention belongs to the field of biopharmaceutical research, and in particular relates to application of anti-inflammatory and analgesic bolus and a preparation thereof in preparation of a medicine for promoting refractory wound healing. According to the application of the anti-inflammatory and analgesic bolus and the preparation thereof in preparation of the medicine for promoting refractorywound healing in the invention, due to animal experiments and immunohistological experiments, researches find that the anti-inflammatory and analgesic bolus has the obvious promoting effect on refractory wound healing; and further researches find that the promoting effect of the anti-inflammatory and analgesic bolus on refractory wound healing is mainly related to effects of reducing abnormal senescence of fibroblasts, resisting oxidization, promoting fibroblast proliferation induced by oxidative damage, promoting cell adhesion, reducing apoptosis of fibroblasts, promoting collagen secretion,etc.

Description

technical field [0001] The invention relates to the field of biomedical research, in particular to the application of Pien Tze Huang and its preparations in the preparation of medicines for promoting the healing of refractory wounds. Background technique [0002] Non-healing wounds are also called chronic wounds or chronic wounds. It is generally believed that non-healing wounds refer to wounds that cannot achieve anatomical and functional damage through a normal, orderly and timely repair process. There is no absolute time limit for wound healing. Usually, when the wound cannot shrink by 10-15% per week or 50% for more than one month, it is considered a chronic wound. Participated in the literature: Jiang Yufeng, Fu Xiaobing. Body Surface Research progress of chronic refractory wounds[J]. Infection, Inflammation, Repair, 2011,12(01):59-61. Common refractory wounds include large-area skin defect wounds, large-area skin avulsion wounds, chronic ulcer wounds, immune chronic w...

Claims

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Application Information

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IPC IPC(8): A61K36/258A61P17/02A61K35/413A61K35/55A61K35/583
CPCA61K35/413A61K35/55A61K35/583A61K36/258A61P17/02A61K2300/00
Inventor 刘建顺黄进明洪绯于娟庄毅超陈志亮闫昳姝王世聪
Owner ZHANGZHOU PIEN TZE HUANG PHARM
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