Ester catechin synthase, coding gene and application of ester catechin synthase

A technology of ester catechin and synthetase, which is applied in the directions of application, genetic engineering, plant genetic improvement, etc., can solve the problem that the formation mechanism of catechin gallate is not determined.

Active Publication Date: 2020-08-25
ANHUI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it is known that there are generally two types of enzymes that catalyze the esterification of such organic acids with flavonoids such as flavantriols: BAHD and SC

Method used

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  • Ester catechin synthase, coding gene and application of ester catechin synthase
  • Ester catechin synthase, coding gene and application of ester catechin synthase
  • Ester catechin synthase, coding gene and application of ester catechin synthase

Examples

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Embodiment

[0034] Example Cloning, expression and detection of enzymatic properties of serine carboxypeptidase acyltransferase gene

[0035]1. Cloning of CsSCPL11-IA, CsSCPL13-IA and CsSCPL14-IA genes

[0036] The primer sequences of the three genes are shown below.

[0037] >CsSCPL11-IA-F

[0038] ATGTTTCCACCAAAGTCATA, SEQ ID NO. 7;

[0039] >CsSCPL11-IA-R

[0040] CTAAATAGGATAGTAATGAA, SEQ ID NO. 8;

[0041] >CsSCPL13-IA-F

[0042] ATGGTGCAAGTAGAAGCCAT, SEQ ID NO. 9;

[0043] >CsSCPL13-IA-R

[0044] AACAGGATTATAATTAATCC, SEQ ID NO. 10;

[0045] >CsSCPL14-IA-F

[0046] ATGGTGCAAGTAGAAGCCAT, SEQ ID NO. 11;

[0047] >CsSCPL14-IA-R

[0048] CTAGGAGTGGTGAGTTAGGT, SEQ ID NO. 12;

[0049] According to the instructions of TIANGEN Plant Total RNA Extraction Kit and M-MLV cDNA Synthesis Kit, the total RNA of Shucha early young leaves was extracted and reverse transcribed into cDNA.

[0050] Using reverse-transcribed cDNA as a template, primers and TAKARA ExTaq polymerase were used fo...

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Abstract

The invention belongs to the technical field of bioengineering, and particularly relates to an ester catechin synthase, a coding gene and application of the ester catechin synthase. A recombinant protein is obtained by constructing a recombinant engineered bacteria, with the help of an enzyme reaction method, a recombinant protein of a genetically engineered bacteria is used as a catalytic enzyme,and genes, encoding the serine carboxypeptidase acylase CsSCPL11-IA, CsSCPL13-IA and CsSCPL14-IA, in tea plants are verified. Three acetyltransferase genes that can catalyze the synthesis of ester catechins and the application values in the breeding of excellent tea varieties and tea processing are described for the first time.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to an ester-type catechin synthase, a coding gene and an application thereof. Background technique [0002] Tea polyphenols is a natural polyphenol compound extracted from tea, accounting for about 25% of the dry weight of tea. It is a general term for more than 30 polyphenol compounds in tea. one of the main medicinal ingredients. Catechins are one of the main components of tea polyphenols, accounting for 50% to 70% of its total weight. The catechins in tea contain multiple components, which are mainly divided into ester type and non-ester type catechin. Non-ester catechins include catechin (C, (+)-catechin), epicatechin (EC, (-)-epicatechin), gallate catechin (GC, (+)-gallocatechin, and epicatechin Epicatechin (EGC, (-)-epigallocatechin). Ester catechins include epicatechin gallate (ECG, (-)-epicatechin-3-gallate) and epigallocatechin-3-gallate Ester (EGCG, ...

Claims

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Application Information

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IPC IPC(8): C12N9/10C12N15/54C12P17/06C12Q1/6895A23F3/06A01G17/00
CPCC12N9/1029C12P17/06C12Q1/6895A23F3/06A01G17/005C12Q2600/13
Inventor 赵剑左飞李鹏辉曾祥圣
Owner ANHUI AGRICULTURAL UNIVERSITY
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