Preparation method and quality detection method of lithocarpus polystachyus rehd total flavone extract

A quality detection method, a technology of total flavonoids, applied to medical preparations containing active ingredients, measuring devices, drug combinations, etc., can solve problems such as lack of quality standards

Pending Publication Date: 2020-08-28
GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Dosuike has not been recorded in the herbal medicines of the past dynasties, but it is scattered in some Chinese herbal medicine monographs in my country and Chinese herbal medicine handbooks or botanical monographs in the south, such as "Flora of China", "Illustrated Book of Higher Plants of China", " Flora of Guizhou, Flora of Yunnan, List of Medicinal Plants in Guangxi, National Compilation of Chinese Herbal Medicine, etc., currently there is no statutory quality standard

Method used

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  • Preparation method and quality detection method of lithocarpus polystachyus rehd total flavone extract
  • Preparation method and quality detection method of lithocarpus polystachyus rehd total flavone extract
  • Preparation method and quality detection method of lithocarpus polystachyus rehd total flavone extract

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Embodiment 1

[0093] A preparation method for the total flavonoids extract of Polycarpus flavonoids, comprising the following steps:

[0094] (1) Methanol solution heating and reflux extraction:

[0095] ① 300 g of dried leaves of Duosuike, pulverized into a coarse powder, added 10 times by weight of methanol with a volume concentration of 70%;

[0096] ② Heating and reflux extraction at 90°C for 2 times, each time for 1.5h, filtering, and combining the filtrates;

[0097] ③ Recover methanol from the filtrate, concentrate until it has no alcohol smell, add water to make up to 5000ml, dilute to the concentration of 0.06g crude drug amount / ml of Duosuike extract, and put it on the column;

[0098] (2) Separation and purification of macroporous adsorption resin:

[0099] ① Select D101 macroporous adsorption resin, filter through four layers of gauze to clear liquid, filter, and pass through D101 macroporous adsorption resin column;

[0100] ②Adsorption: The concentration of the extract of t...

Embodiment 2

[0104] A preparation method for the total flavonoids extract of Polycarpus flavonoids, comprising the following steps:

[0105] (1) Methanol solution heating and reflux extraction:

[0106] 1. 100 g of dried leaves of Duosuike, pulverized into a coarse powder, and adding 15 times of methanol with a volume concentration of 80% by weight;

[0107] ② Heat and reflux extraction at 80°C for 4 times, 1 hour each time, filter, and combine the filtrates;

[0108] ③ Recover methanol from the filtrate, concentrate until it has no alcohol smell, add water to dilute to 2000ml, dilute to a concentration of 0.05g crude drug amount / ml of Duosuike extract, and put it on the column;

[0109] (2) Separation and purification of macroporous adsorption resin:

[0110] ① Select non-polar XAD-1600N macroporous adsorption resin column, filter with four layers of gauze to clear liquid, filter, and pass through XAD-1600N macroporous adsorption resin column;

[0111] ②Adsorption: The concentration of...

Embodiment 3

[0115] A preparation method for the total flavonoids extract of Polycarpus flavonoids, comprising the following steps:

[0116] (1) Methanol solution heating and reflux extraction:

[0117] ① 50 g of dried leaves of Duosuike, pulverized into a coarse powder, added 5 times by weight of methanol with a volume concentration of 90%;

[0118] ② Heat and reflux extraction at 80°C for 3 times, each time for 0.5h, filter and combine the filtrates;

[0119] ③ Recover methanol from the filtrate, concentrate until it has no alcohol smell, add water to make up to 1000ml, dilute to 0.05g crude drug amount / ml of the extract of Duosuikee, and wait for column use;

[0120] (2) Separation and purification of macroporous adsorption resin:

[0121] ① Select XAD-18 macroporous adsorption resin column with medium polarity, filter through four layers of gauze to clear liquid, filter, and pass through XAD-18 macroporous adsorption resin column;

[0122] ②Adsorption: The concentration of the extra...

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Abstract

The invention relates to a preparation method and a quality detection method of lithocarpus polystachyus rehd total flavone extract. The preparation method of the lithocarpus polystachyus rehd total flavone comprises the following steps: drying leaves of lithocarpus polystachyus rehd, crushing, heating with methanol or ethanol for reflux extraction, concentrating, recovering methanol or ethanol, adding water into crude extract for suspension, performing macroporous adsorption resin column chromatography, eluting, concentrating and drying to obtain the lithocarpus polystachyus rehd total flavone. The preparation process of the total flavone extract in lithocarpus polystachyus rehd is researched, the quality standard of the total flavonoid extract in lithocarpus polystachyus rehd is researched, and a reference basis is provided for establishing the complete quality standard of lithocarpus polystachyus rehd and developing and utilizing resources in future. Pharmacological experiments prove that the lithocarpus polystachyus rehd total flavone extract prepared by the method has a good hypoglycemic effect and can be applied to preparation of hypoglycemic drugs.

Description

technical field [0001] The invention relates to the technical field of preparation of extracts of traditional Chinese medicines, in particular to a preparation method and a quality detection method of the total flavonoids extract of Polyacopteris flavonoids. Background technique [0002] Duosuike, also known as Mujiangyeke, Duozhenshike, and sweet leaf tree, is a plant of the Fagaceae Mujiangyeke ( Lithocarpus litseifolius (Hance) Chun. ) dry leaves, which are commonly used as tea in Guangxi folks, and have the effects of lowering blood sugar, anti-oxidation, lowering blood pressure, antibacterial, anti-allergic, and anti-cancer. It is not recorded in the herbal medicines of the past dynasties, but it is scattered in some Chinese herbal medicine monographs in my country and Chinese herbal medicine handbooks or botanical monographs in the south, such as "Flora of China", "Illustrated Book of Higher Plants of China", " Guizhou Flora, Yunnan Flora, Guangxi Medicinal Plant Catal...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/90G01N30/94G01N21/33A61P3/10A61K36/185
CPCG01N30/02G01N30/06G01N30/90G01N30/94G01N21/33A61K36/185A61P3/10A61K2236/15A61K2236/333A61K2236/55A61K2236/51G01N2030/027G01N2030/062
Inventor 袁健童刘布鸣柴玲陈明生冯军
Owner GUANGXI INST OF CHINESE MEDICINE & PHARMA SCI
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