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Application of Cotton ghpsat2 Gene in Promoting Plant Flowering

A gene and plant technology, applied in the field of application of GHPSAT2 gene in promoting plant flowering, can solve problems such as few studies

Active Publication Date: 2021-12-14
INST OF COTTON RES CHINESE ACAD OF AGRI SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The PSAT2 gene belongs to the aspartate aminotransferase family and is currently poorly studied in plants

Method used

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  • Application of Cotton ghpsat2 Gene in Promoting Plant Flowering
  • Application of Cotton ghpsat2 Gene in Promoting Plant Flowering
  • Application of Cotton ghpsat2 Gene in Promoting Plant Flowering

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0023] The following examples are used herein to demonstrate preferred embodiments of the invention. It should be appreciated by those of skill in the art that the techniques disclosed in the examples which follow represent techniques discovered by the inventors to be employed in the practice of the invention, and thus can be considered preferred modes for its practice. However, those skilled in the art should understand from this specification that many modifications can be made to the specific embodiments disclosed herein, and the same or similar results can still be obtained without departing from the spirit or scope of the present invention.

[0024] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which this invention belongs, and the disclosures cited herein and their cited materials are all incorporated by reference .

[0025] Those skilled in the art will recognize, or ...

Embodiment PBI121

[0035] The construction of embodiment PBI121-GhPSAT2 expression vector

[0036] 1 Obtain the gene sequence of GhPSAT2 (Gh_D07G1721) from CottonFGD, use Oligo7 software to design primers, and use PCR method to amplify from No. 50 of upland cotton Zhongmian Institute. Its CDS sequence is 1263bp long and encodes 420 amino acids. The relative molecular weight of the protein It is 46.26213kDa, and its isoelectric point is 8.52.

[0037] Amplification primers are as follows:

[0038] Upstream primer F (SEQ ID No.1): 5'-ATGGCAGCAACATCTCTAAAT-3'

[0039] Downstream primer R (SEQ ID No.2): 5'-TCAAGCATGCTTTGCCTGGAA-3'

[0040] The open reading frame sequence is (SEQ ID No.3):

[0041]ATGGCAGCAACATCTCTAAATGCCCCTAACGCTCCTCTCCTTCAAAAGACCCATCAAACTCATGTCTTTCTCAAACCCATCTCCACCATTCCTTGTCAAACCTCTGCCAAGCGCTTCTCCATCTCTTGTTCCGCAACTACCCAAGATCGCCTCTCCGTCCAATCCCAATCTCAAGATCGGGTCTTTAACTTCGCCGCCGGTCCCGCCACCTTACCCGAGAACGTCCTCCTCAAAGCCCAATCCGAGCTTTACAACTGGCACGGATCTGGCATGAGCGTTATGGAAATGAGCCACCGTGGTAAGGA...

Embodiment 2

[0145] Transformation of Arabidopsis thaliana mediated by embodiment 2 Agrobacterium

[0146] Transformation of Arabidopsis thaliana by inflorescence dipping method

[0147] 1) Inoculate 20 μL of the Agrobacterium liquid cultured at -20°C into 1 mL of LB liquid medium and culture overnight at 28°C with shaking at 180 rpm. Take 200 μL of the activated bacteria liquid and add it to 50 mL of LB liquid medium for shaking at 28°C at 180 rpm.

[0148] 2) OD of the bacteria solution 600 When the value is about 1.2, centrifuge at 5,000rpm for 5min to collect the bacteria.

[0149] 3) Discard the supernatant, and use 50-70ml transformation medium to reselect the bacterial solution to make the OD 600 Infection begins after the value is 0.8-1.0 (transformation medium formula: 0.217g 1 / 2MS, 5g sucrose, 100mL water dissolved and then added 20μL silwetL-77).

[0150] 4) Select unflowered Arabidopsis thaliana, place the Arabidopsis inflorescences in the transformation medium for 30-50 sec...

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Abstract

The invention discloses cotton GHPSAT2 The application of genes in promoting plant flowering belongs to the technical field of plant genetic engineering. GHPSAT2 The gene has the nucleotide sequence shown in SEQ ID No.3 and can encode the amino acid sequence shown in SEQ ID No.4. The transgene obtained by the present invention through transgenic technology GHPSAT2 Arabidopsis plants with genes that lead to a significantly earlier flowering time of Arabidopsis, indicating that GHPSAT2 Gene plays key role in promoting flowering in cotton. The invention provides favorable gene resources for short-season cotton cultivation.

Description

technical field [0001] The invention belongs to the technical field of plant genetic engineering, and in particular relates to the application of GHPSAT2 gene in promoting flowering of plants. Background technique [0002] Cotton is an important economic crop and textile raw material in my country, and plays an irreplaceable role in the development of China's national economy. However, my country has a large population and a relatively small area of ​​arable land, and the contradiction between grain and cotton competing for land is prominent. Early-maturing cotton is a cotton type that is suitable for alleviating the current situation of land competition for grain and cotton and the gradual reduction of vegetation area. Taking advantage of its short growth period and late sowing characteristics, it can achieve double cropping of grain and cotton in one year with winter crops, improve the multiple cropping index of land, expand cotton planting areas, avoid seedling pests and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/54C12N9/10C12N15/82A01H5/00A01H5/02A01H6/20
CPCC12N9/1096C12N15/827C12Y206/01001
Inventor 喻树迅程小倩魏恒玲李林王寒涛马亮付小康
Owner INST OF COTTON RES CHINESE ACAD OF AGRI SCI
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