Detection method and application of correlation between garnl1 gene and rooster comb and wattle traits

A correlation and rooster technology, which is applied in the field of detection of the correlation between GARNL1 gene and rooster comb and wattle traits, achieving the effect of low cost, strong repeatability and accurate results

Active Publication Date: 2021-11-16
NANCHANG NORMAL UNIV
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Problems solved by technology

Shen et al. (2012) found that the rs16492031 locus was extremely significantly correlated with the hen's first laying age (P<0.01), but whether this locus is related to the cockscomb and wattle traits of roosters has not been reported

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  • Detection method and application of correlation between garnl1 gene and rooster comb and wattle traits
  • Detection method and application of correlation between garnl1 gene and rooster comb and wattle traits
  • Detection method and application of correlation between garnl1 gene and rooster comb and wattle traits

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Embodiment Construction

[0019] Various exemplary embodiments of the present invention will now be described in detail. The detailed description should not be considered as a limitation of the present invention, but rather as a more detailed description of certain aspects, features and embodiments of the present invention.

[0020] It should be understood that the terminology described in the present invention is only used to describe specific embodiments, and is not used to limit the present invention. In addition, regarding the numerical ranges in the present invention, it should be understood that each intermediate value between the upper limit and the lower limit of the range is also specifically disclosed. Each smaller range between any stated value or intervening value in a stated range and any other stated value or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded from the range. ...

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Abstract

The invention discloses a detection method and application of the correlation between GARNL1 gene and rooster comb and wattle traits, belonging to the field of genetic engineering. The method first extracts the genomic DNA of the chicken species to be detected, and then amplifies the GARNL1 gene intron 32 The 752bp fragment upstream and downstream of the rs16492031 site, and then use the RFLP type of the obtained PCR product, and then statistically analyze the correlation between the different genotypes of the GARNL1 gene site rs16492031 and the cock comb and wattle traits of the rooster; The relationship between the polymorphism of the rs16492031 site in the GARNL1 gene and the correlation between the rooster comb and wattle traits, and using this relationship and method as a means of breeding and marking chicken breeds. Compared with the current generation selection, this breeding method is not only low in cost, easy to operate, but also accurate in results.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a detection method and application of the correlation between GARNL1 gene and rooster comb and wattle traits. Background technique [0002] In domestic chicken breeding, the traits of precocity are mainly selected from the age at first production, the development of comb and wattle. Zhang Dexiang (1999) researched that it is feasible to select the early maturity of high-quality chickens with the comb area as the index. Wei Fengying et al. (2002) used comb area as the main indicator to carry out early-maturity trait selection in Yinxiang Ma chickens, and found that the breeding age of roosters after 5 generations was advanced by 23 days, and the age of female hens was advanced by 15 days. 400 Day-old egg production increased by 20 eggs. Ji Congliang et al. (2008) found that there is a correlation between early comb height and sexual maturity, and there is a correlation between...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 周敏谭玉文朱学农许继国贡继尚文正亚
Owner NANCHANG NORMAL UNIV
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