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Method for determining residual quantity of ribavirin and amantadine compounds in poultry food

An amantadine and compound technology, which is applied in the field of determination of ribavirin and amantadine compound residues in poultry food, and achieves the effects of high sensitivity and accurate quality.

Pending Publication Date: 2020-09-11
ANHUI PROVINCIAL CENT FOR DISEASE CONTROL & PREVENTION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few relevant research reports on the detection of total residues of ribavirin and amantadine compounds in animal-derived foods in my country, and most of them use liquid chromatography-tandem mass spectrometry to detect ribavirin or amantadine compounds

Method used

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  • Method for determining residual quantity of ribavirin and amantadine compounds in poultry food
  • Method for determining residual quantity of ribavirin and amantadine compounds in poultry food
  • Method for determining residual quantity of ribavirin and amantadine compounds in poultry food

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Embodiment 1

[0026] Example 1: A method for determining the residues of ribavirin and amantadine compounds in poultry food

[0027] 1. Main instruments and reagents.

[0028] Acquity in Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry TM UPLC Ultra High Performance Liquid Chromatograph, XEVO TM TQMS were purchased from Waters, USA, Milli-Q ultrapure water system (Millipore, USA), vortex mixer, electronic balance, GX-271 solid phase extraction instrument (Gilson, USA), high-speed refrigerated centrifuge, MTN-2800D nitrogen blowing concentration Device, phenylboronic acid (PBA) / polymeric cation exchange resin (PCX) composite solid-phase extraction column (self-made, 200mg / 100mg, 3ml, PBA and PCX filler: American Agilent). A piece of polyethylene sieve plate (pore size: 20 μm) was pressed into the bottom of the empty polypropylene solid-phase extraction column tube (3mL type), and 100 mg of polymeric cation exchange resin filler (PCX) was quantitatively loaded into the col...

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Abstract

The invention discloses a method for determining the residual quantity of ribavirin and amantadine compounds in poultry food. The method comprises: establishing an ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS / MS) method to detect the residual quantity of ribavirin and amantadine compounds in poultry food; performing enzymolysis on a sample, trichloroacetic acid precipitating proteins, performing low temperature high speed centrifugation, adjusting the pH value of the supernatant, and purifying through a PBA / PCX composite solid-phase extraction column; analyzing ribavirin by virtue of an Agilent ZORBAX SB-Aq column (3.0 mm * 100mm, 1.8 mu m), analyzing amantadine by virtue of a Waters BEH C18 chromatographic column (2.1 mm * 100mm, 1.7 mu m), measuring by virtue of tandem mass spectrometry, and quantifying by virtue of an isotope internal standard method. The method has the characteristics of simplicity, convenience, quickness, high sensitivity, accuracy in qualitative analysis and the like.

Description

technical field [0001] The invention belongs to the technical field of detection, and relates to a method for measuring residual amounts of ribavirin and amantadine compounds in poultry food. Background technique [0002] Ribavirin, also known as ribavirin, is a broad-spectrum potent synthetic nucleoside antiviral drug, which has inhibitory effects on DNA and RNA viruses, and is mainly used for human viral influenza, pneumonia, hepatitis A and Treatment of diseases such as viral encephalitis. Amantadine compounds mainly include amantadine, adamantanemethylamine, rimantadine and 3,5-dimethylamantadine, among which amantadine is mainly used for Inhibiting Asian influenza A virus; amantadine is superior to amantadine in anti-A2 virus and parainfluenza virus; amantadine is also stronger in inhibiting type A influenza virus than amantadine, but the central nervous side effects are weak In amantadine; 3,5-dimethylamantadine, also known as memantine, is commonly used clinically i...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/88
CPCG01N30/06G01N30/88
Inventor 谢继安刘柏林赵紫微吴翠玲
Owner ANHUI PROVINCIAL CENT FOR DISEASE CONTROL & PREVENTION
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