A kind of highly organic sulfur-tolerant Thioalkali halophilus and its cultivation method and application in biological desulfurization

A biological desulfurization and organic sulfur technology, applied in the direction of microorganism-based methods, microorganisms, microorganisms, etc., can solve the problems of low tolerance, difficult treatment, and restrictions on the promotion and application of technologies, so as to improve tolerance and expand the scope of application Effect

Active Publication Date: 2022-05-20
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the biological desulfurization process, organic sulfur will seriously inhibit the activity of desulfurization bacteria and reduce the HS - The oxidation rate, resulting in HS - Accumulation leads to the collapse of the desulfurization system
The currently used neutrophil or alkalophilic sulfur-oxidizing bacteria are not highly tolerant to organic sulfur, which makes it difficult for biological desulfurization technology to treat gases with high organic sulfur content, which restricts the popularization and application of the technology

Method used

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  • A kind of highly organic sulfur-tolerant Thioalkali halophilus and its cultivation method and application in biological desulfurization
  • A kind of highly organic sulfur-tolerant Thioalkali halophilus and its cultivation method and application in biological desulfurization
  • A kind of highly organic sulfur-tolerant Thioalkali halophilus and its cultivation method and application in biological desulfurization

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] This example provides a method for screening and isolating Thioalkali halophilus BDH06, which is highly tolerant to organic sulfur, and the operation is as follows:

[0064] (1) Collect the sediments from the shore of the saline-alkali lake in Badain Jaran Desert, Inner Mongolia, take 1g of the sample and place it in 100mL of sodium thiosulfate liquid medium, and culture it on a shaking table at 30°C and 150rpm for 60h;

[0065] (2) Take 1 mL of the suspension and inoculate it into 100 mL of sodium thiosulfate liquid medium, and continue to culture at 30°C and 150 rpm on a shaker for 60 h;

[0066] (3) Take 100 μL of the suspension and spread it on the surface of the sodium thiosulfate solid medium, culture it at 30°C for 60 hours, and select white colonies for re-screening and identification;

[0067] Wherein, sodium thiosulfate liquid culture medium comprises following composition: 0.05mmol / L methyl mercaptan, 30g / L Na 2 S 2 o 3 , 30g / L NaHCO 3 , 5g / L NaOH, 10g / L ...

Embodiment 2

[0076] This example compares the tolerance to methyl mercaptan of Thioalkalibacterium halophilus BDH06 described in the present invention and the sulfoalkali vibrio pluripotent D301 (strain number CGMCC No.8497) disclosed in the patent No. CN201410061877.1, the operation is as follows :

[0077] Take 1 mL of the two activated bacterial solutions and inoculate them in sodium thiosulfate liquid medium (100 mL, pH=9.5, sodium ion concentration: 1.0 M) containing different concentrations of methyl mercaptan respectively, shake on a shaker at 30°C and 150 rpm to cultivate. The final concentrations of methyl mercaptan in the culture medium were 0, 0.01mmol / L, 0.05mmol / L, 0.1mmol / L, 0.2mmol / L, 0.4mmol / L, 1.0mmol / L, 2.0mmol / L. After culturing for 48 hours, samples were taken, and the utilization rate of sodium thiosulfate was determined by ion chromatography.

[0078] The result is as image 3 Shown, the inhibition coefficient IC of methyl mercaptan to Thioalkali halophilus BDH06 ...

Embodiment 3

[0081] In this example, Thioalkali halophilus BDH06 is used for the treatment of hydrogen sulfide-containing gas, and the operation is as follows:

[0082] (1) Prepare the test gas containing hydrogen sulfide, which contains 5000ppm hydrogen sulfide and the rest is nitrogen.

[0083] (2) The hydrogen sulfide-containing gas is countercurrently passed through the absorption tower, during which the gas is in contact with the lye, wherein the hydrogen sulfide is absorbed by the lye to form a high-sulfur solution containing sodium hydrogen sulfide; the high-sulfur solution enters the bioreactor; In the reactor, by controlling the oxidation-reduction potential, Thioalkali halophilus BDH06 oxidizes hydrogen sulfide to elemental sulfur, and at the same time regenerates the lye for recycling;

[0084] The above-mentioned lye is a buffer solution prepared by sodium bicarbonate and sodium hydroxide, and the composition includes 30g / L NaHCO 3 , 5g / LNaOH, 10g / L NaCl, 0.5g / L NH 4 Cl, 0.5g...

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Abstract

The invention relates to a highly tolerant organic sulfur halophilic bacillus, the high organic sulfur tolerant halophilic bacillus is named as the BDH06 strain of Thioalkalibacter halophilus, and the preservation unit is China Microbial Bacteria The General Microbiology Center of the Species Preservation Management Committee, the preservation time is June 10, 2020, the preservation number is CGMCC No. 20058, and the address is: No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. The strain has high tolerance to organic sulfur, and has the ability to oxidize sulfur hydride to generate elemental sulfur under the conditions of high salt, high alkali and high organic sulfur. The yield of elemental sulfur is over 80%, and the removal rate of hydrogen sulfide It reaches more than 99%, which significantly improves the tolerance of the system to organic sulfur and expands the application range of biological desulfurization technology.

Description

technical field [0001] The invention belongs to the technical field of microorganism isolation and cultivation, and in particular relates to a thioalkalibacterium halophilus and its cultivation method and its application in biological desulfurization, in particular to a thioalkalibacterium halophilus with high tolerance to organic sulfur and its cultivation method and Application in biological desulfurization. Background technique [0002] Biological desulfurization technology can remove H in gas at normal temperature and pressure 2 S is removed and elemental sulfur product is generated. Compared with traditional physical and chemical methods, biological desulfurization technology has the advantages of mild conditions, no secondary pollution, and no clogging of the system. It has been used in natural gas, biogas and other desulfurization projects. The desulfurization bacteria used in this technology are mainly chemoautotrophic microorganisms that can utilize CO 2 as a car...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B01D53/48B01D53/52B01D53/78B01D53/96C10L3/10C02F101/10C02F103/18C12R1/01
CPCC12N1/20C02F3/345B01D53/485B01D53/48B01D53/52B01D53/78B01D53/96C10L3/103C02F2101/101C02F2103/18B01D2257/306B01D2258/05C12R2001/01C12N1/205Y02P20/59
Inventor 杨茂华邢建民穆廷桢苗得露
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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