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A kind of γ-polyglutamic acid-polycation complex and its preparation method and application

A technology of polycation and polyglutamic acid, which is applied in the field of γ-polyglutamic acid-polycation complex and its preparation, can solve the problem of difficult to stably adhere to the surface of medical devices, poor persistent antibacterial effect, and affecting biocompatibility In order to achieve the effects of inhibiting adhesion, non-irritating solvent tissue, and simple and easy preparation conditions

Active Publication Date: 2021-06-15
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the single-constructed hydrophilic coating has the disadvantages of relatively single function and poor persistent antibacterial effect.
[0003] The invention with the publication number CN 105268015 B discloses an antibacterial hydrogel composite material and its preparation method. The antibacterial wound repair material it provides is a kind of chitosan and The mass ratio of -2 is a hydrogel formed by electrostatic interaction, and nano-silver is synthesized in situ on the hydrogel with PVP as a dispersant; the wound repair material has acetic acid added during the gelation process, which affects Biocompatibility, the follow-up purification process is relatively cumbersome, and the formed gel has poor anchoring properties, making it difficult to stably adhere to the surface of medical devices, and there are disadvantages in practical applications

Method used

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  • A kind of γ-polyglutamic acid-polycation complex and its preparation method and application
  • A kind of γ-polyglutamic acid-polycation complex and its preparation method and application
  • A kind of γ-polyglutamic acid-polycation complex and its preparation method and application

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] (1) Dissolve γ-PGA powder (with a molecular weight of 5000 Daltons) in water to obtain a polyanion solution with a concentration of 20 g / L; dissolve ε-PL powder (with a molecular weight of 2500 Daltons) in water to obtain a concentration of It is a 40g / L polycation solution.

[0038] (2) Mix and stir the polyanion solution and the polycation solution according to the mass ratio of γ-PGA to ε-PL of 1:2. The stirring rate of mixing and stirring at room temperature is controlled at 80rpm / min, and the time is 2min. After suction filtration, a white insoluble γ-PGA-ε-PL complex precipitate was obtained.

[0039] (3) Centrifuge the γ-PGA-ε-PL complex precipitate obtained in step (2), wash with water and then vacuum-dry to obtain γ-PGA-ε-PL complex powder.

[0040] (4) Dissolve the γ-PGA-ε-PL composite powder in an ethanol solution to prepare a solution with a mass concentration of 1 wt%, and then apply it to the surface of a commercial medical polyethylene implant, and then ...

Embodiment 2

[0042] (1) γ-PGA powder (molecular weight is 5000 Daltons) is dissolved in water to obtain a polyanion solution with a concentration of 20g / L; carboxymethyl chitosan powder (molecular weight is 2500 Daltons) is dissolved in water , to obtain a concentration of polycation solution of 40g / L.

[0043] (2) Mix and stir the polyanion solution and the polycation solution according to the mass ratio of γ-PGA and carboxymethyl chitosan as 1:2, the stirring rate of normal temperature mixing and stirring is controlled at 80rpm / min, and the time is 2min. Type vacuum water pump suction filtration, obtain white insoluble γ-PGA-carboxymethyl chitosan complex precipitation.

[0044] (3) The γ-PGA-carboxymethyl chitosan complex precipitate obtained in the step (2) is centrifuged, washed with water and then vacuum-dried to obtain γ-PGA-carboxymethyl chitosan complex powder.

[0045] (4) γ-PGA-carboxymethyl chitosan composite powder is dissolved in ethanol solution, is made into the solution t...

Embodiment 3

[0047] (1) γ-PGA powder (molecular weight is 5000 Daltons) is dissolved in water to obtain a polyanion solution with a concentration of 20g / L; chitosan quaternary ammonium salt powder (molecular weight is 2500 Daltons) is dissolved in water , to obtain a concentration of polycation solution of 40g / L.

[0048] (2) Mix and stir the polyanion solution and the polycation solution according to the mass ratio of γ-PGA and chitosan quaternary ammonium salt as 1:2. Type vacuum water pump suction filtration, obtains the white insoluble γ-PGA-chitosan quaternary ammonium compound precipitate.

[0049] (3) Centrifuge the precipitate of the γ-PGA-chitosan quaternary ammonium salt complex obtained in step (2), wash with water and then vacuum-dry to obtain γ-PGA-chitosan quaternary ammonium salt complex powder.

[0050] (4) γ-PGA-chitosan quaternary ammonium salt complex powder is dissolved in ethanol solution, is made into the solution that mass concentration is 1wt%, is coated on the sur...

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Abstract

The invention discloses a γ-polyglutamic acid-polycation complex and its preparation method and application. A polyamino acid complex formed by interaction; the γ-polyglutamic acid is obtained by microbial fermentation, with a molecular weight range of 5000 to 10000 Daltons, preferably 5000 Daltons; the polycationic compound is polylysine, Any one of carboxymethyl chitosan, chitosan quaternary ammonium salt, polylysine hydrochloride. The composite is used as an antifouling coating on the surface of a medical device, which can stably adhere to the surface of the medical device, has a long acting time, and effectively isolates the growth of microorganisms on the surface of the device, and inhibits the adhesion of microorganisms on the surface.

Description

technical field [0001] The invention belongs to the field of antibacterial technology, and in particular relates to a gamma-polyglutamic acid-polycation complex and its preparation method and application. Background technique [0002] Contamination of medical devices during surgery often leads to failure and reduced service life of medical devices. The risk of uncontrollable contamination of medical devices greatly increases the potential risk and cost of surgery during surgery. Implantable medical devices, such as medical catheters and intravenous catheters, are also restricted in use because of the risk of contamination. Studies have found that medical devices are easily adhered by bacteria to form biofilms on their surfaces, and contaminated medical devices are the key cause of bacterial infection and the formation of drug resistance. At present, for the mechanism of biofilm formation, the common antifouling method for medical devices is to graft some antifouling compou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09D177/04C09D105/08C09D5/16
CPCC08L2203/02C08L2203/18C08L2205/025C09D5/1662C09D105/08C09D177/04C08L77/04
Inventor 徐虹王瑞韩伟李莎雷鹏罗正山
Owner NANJING TECH UNIV