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Device for separating high-density lipoprotein from serum, preparation method of device and method for separating high-density lipoprotein from serum

A technology of high-density lipoprotein and low-density lipoprotein, which is applied to the preparation method of peptides, chemical instruments and methods, animal/human peptides, etc., can solve the problems of many experimental steps, expensive, time-consuming, etc., and achieve easy The effect of preparation, low equipment cost and convenient operation

Pending Publication Date: 2020-09-29
LUMIGENEX (SUZHOU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] The currently widely used ultracentrifugation method for separating LDL and HDL requires the use of an expensive ultrahigh-speed centrifuge as the main separation equipment, and there are many experimental steps, the operation is very complicated, and a variety of reagents need to be prepared. Most importantly, it is very expensive. When the total separation time is often more than 6 hours

Method used

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  • Device for separating high-density lipoprotein from serum, preparation method of device and method for separating high-density lipoprotein from serum
  • Device for separating high-density lipoprotein from serum, preparation method of device and method for separating high-density lipoprotein from serum
  • Device for separating high-density lipoprotein from serum, preparation method of device and method for separating high-density lipoprotein from serum

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Embodiment 1: include a pipe body with a pipe and the two ends of the pipe communicate with the outside world, a filter assembly arranged in the pipe of the pipe body, the filter assembly includes a low-density lipoprotein barrier filter membrane:

[0057] (1) Cutting the GFB glass fiber filter membrane into discs with a diameter of 13 mm for subsequent use;

[0058] (2) Prepare 15mL treatment solution, including: dextran sulfate (DS) sodium salt (6,500-10,000): 25mg / ml, MgCl 2 :0.25M, Procline300: mass percentage 0.02%;

[0059] (3) Soak the circular filter membrane produced in the first step in the treatment solution for 1 hour, and dry it in a blast oven for 1 hour at 37 degrees Celsius to prepare a low-density lipoprotein barrier filter membrane 1, which is set aside;

[0060] (4) Insert the prepared four round low-density lipoprotein barrier filter membranes 1 into the 5mL pipette tip 2, and press the low-density lipoprotein barrier filter membrane 1 in the pipett...

Embodiment 2

[0063] Embodiment 2: include a pipe body with a pipe and the two ends of the pipe communicate with the outside world, a filter assembly arranged in the pipe of the pipe body, and the filter assembly includes a low-density lipoprotein barrier filter membrane:

[0064] (1) Cutting the Millipore glass fiber filter membrane into discs with a diameter of 13 mm for subsequent use;

[0065] (2) Prepare 15mL treatment solution, including: sodium phosphotungstic acid: 10mg / ml, MgCl 2 :0.25M, Procline300: mass percentage 0.02%;

[0066] (3) Soak the circular filter membrane produced in the first step in the treatment solution for 1 hour, and dry it in a blast oven for 1 hour at 37 degrees Celsius to prepare a low-density lipoprotein barrier filter membrane 1, which is set aside;

[0067] (4) Insert the prepared four round low-density lipoprotein barrier filter membranes 1 into the 5mL pipette tip 2, and press the low-density lipoprotein barrier filter membrane 1 in the pipette tip 2 ti...

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Abstract

The invention relates to a device for separating a high-density lipoprotein from serum. The device comprises a pipe body and a filter assembly, wherein the pipe body is provided with a pipeline and two ends of the pipeline communicate with the outside; the filter assembly is arranged in the pipe body and comprises a low-density lipoprotein blocking filter membrane; the low-density lipoprotein blocking filter membrane is prepared by soaking the filter membrane in a treating fluid and then drying the filter membrane; and the treating fluid comprises a low-density lipoprotein blocking reagent with the concentration of 20-30 mg / mL, MgCl2 with the concentration of 0.2-0.3 M, a preservative with the mass concentration of 0.016%-0.024% and water. The device is low in equipment cost, high in popularity rate, easy to prepare and obtain, rapid in reaction and convenient to operate.

Description

technical field [0001] The invention relates to a device for separating high-density lipoprotein in serum, a preparation method thereof and a method for separating high-density lipoprotein in serum. Background technique [0002] High-density lipoprotein (High Density Lipoprotein, HDL) is the most lipoprotein in the plasma of most mammals, but the level of HDL in human plasma is lower than that of high-density lipoprotein (LDL), which can be further divided into HDL1, HDL2, HDL according to the density difference Several subclasses such as HDL3. The apolipoproteins in plasma HDL are mainly apoAI and apoAⅡ, the combination of apoAI and HDL is loose, and the combination of apoAⅡ is tight. HDL is a spherical lipoprotein with the highest density, smallest particle size and extremely heterogeneity in plasma. The main function of HDL is to participate in reverse cholesterol transport (RCT) in the body, which can move cholesterol from surrounding tissue cells and transport it to t...

Claims

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Application Information

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IPC IPC(8): C07K14/47C07K1/34
CPCC07K14/47
Inventor 何爱民
Owner LUMIGENEX (SUZHOU) CO LTD
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