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Gene with Salt Tolerance Function and Its Application
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A salt-tolerant and salt-tolerant technology, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems such as water shortage
Active Publication Date: 2021-04-13
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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Problems solved by technology
For our country, the area of saline-alkali land is huge, the soil has high salt content, but it is often short of water.
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Embodiment 1
[0041] Example 1 Screening of Salt Tolerance Genes
[0042] 1. Experimental materials
[0043] The extremely salt-tolerant variety Gehong (GH, 00000601, Hebei) and the extremely salt-sensitive variety Brown Sorghum (HGL, 00013200, Guizhou) at the seedling stage.
[0044] 2. Experimental method
[0045] 2.1 Handling of materials
[0046] Select the seeds of salt-tolerant varieties (00000601) and salt-sensitive varieties (00013200) at the seedling stage, put vermiculite into the seedling tray, and completely infiltrate it with distilled water. After the seeds are sterilized, they are planted into the seedling tray. In the light incubator, at a constant temperature of 25°C, a day and night light cycle of 12h / 12h, 70% humidity, and light intensity of 200μmol·m -2 ·s -1 Cultivate under conditions, the seeds germinate in about 7 days, and irrigate with 1 / 2 Hoagland nutrient solution. Repeat three times.
[0047] 2.2 Transcriptome sequencing
[0048] The extraction, purificati...
Embodiment 2
[0100] Example 2 Response of Salt-tolerant Genes to Salt Stress
[0101] 1. Material handling
[0102] Select the seeds of the salt-sensitive variety (00013200), put vermiculite into the seedling tray, and completely infiltrate it with distilled water. After the seeds are sterilized, they are planted into the seedling tray at a planting depth of about 1 cm, and placed in a light incubator at a constant temperature of 25°C. , Day and night light cycle is 12h / 12h, 70% humidity, light intensity 200μmol m -2 ·s -1 Cultivate under the same conditions, the seeds germinate in about 7 days, and water with 1 / 2 Hoagland nutrient solution. When the seedlings grow to the stage of four leaves and one heart, start to use -1 The seedlings were treated with NaCl solution stress, and the stress treatment time gradient was set as 0h (CK), 6h, 24h, 48h, respectively, and each treatment was repeated three times. In order to maintain the concentration of salt stress, the NaCl solution was renew...
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Abstract
The present invention relates to a gene with salt tolerance function and application thereof, the gene is LOC8075699. The present invention provides a method for identifying plants with salt tolerance and a method for improving the salt tolerance of plants. The invention provides the application of LOC8075699 in identification of salt-tolerant plants, improvement of plant salt tolerance and improvement of salt-tolerant germplasm resources.
Description
technical field [0001] The invention belongs to the field of biotechnology, and relates to a gene with salt-tolerant function and its application. Background technique [0002] The energy problem is the main problem in the world today, and the energy shortage is becoming more and more serious. Therefore, the research, development and production of biomass energy are of great significance to my country's future economic development, technological innovation and development, and national stability. For our country, the area of saline-alkali land is huge, the soil has high salt content, but it often lacks water. Therefore, how to develop and utilize a large area of salinized land and increase crop yield is an important problem that needs to be solved urgently. In nature, the growth process of plants is affected by many abiotic stresses, among which salt stress is the main abiotic stress and one of the main factors causing crop yield reduction. [0003] Salt stress inhibit...
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