Triglyceride detection reagent, detection test paper and test paper preparation method

Abstract

The invention relates to a triglyceride detection reagent, detection test paper and a test paper preparation method. The detection reagent is prepared from 0.5 to 1.5 KU / ml of lipoprotein lipase, 0.3to 0.5 KU / ml of diaphorase, 0.32 to 0.94 KU / ml of glycerol dehydrogenase, 5 to 15mmol / L of coenzyme I and 2 to 6mmol / L of a chromogenic substance, and further comprises a TritonX-100 solution with themass fraction being 0.2%-1.0% and a buffer solution with the concentration being 0.01 mol / L to 0.1 mol / L; wherein the chromogenic substance is one of nitro tetrazole blue chloride, dimethyl thiazolediphenyl tetrazole bromine blue, sodium dichlorophenol indophenolate, TNBT and iodonitrotrtrazolium chloride, the detection test paper comprises a reaction bottom layer, a reaction layer, a blood filtering layer, a capillary sample introduction layer and a hydrophilic layer, and the detection reagent is located on the upper surface of the reaction layer. The interference influence of other substances on the test result is small, and the reagent detection is more stable.

Description

technical field

[0001] The invention relates to the technical field of triglyceride detection, in particular to a triglyceride detection reagent, a detection test paper and a preparation method for the test paper. Background technique

[0002] The currently widely used dry chemical test method for triglycerides is to use lipoprotein esterase, glycerol kinase, glycerol phosphate oxidase coupled Trinder reaction. However, the redox chromogen in this system is very unstable when exposed to air or light, and if it is not stored properly, it is easy to oxidize in advance and change color before the product is used, resulting in product failure or inaccurate test results.

[0003] Existing solutions such as patent CN105295441B add various additives such as color developer stabilizer, reducing agent, chelating agent, auxiliary stabilizer, etc. to the formula, so that the color developer can maintain better color rendering ability; patent CN102128919B also uses Two kinds of color s...

Examples

preparation example Construction

[0044] The above-mentioned preparation method of the detection test paper added with the detection reagent of triglycerides, the specific steps are:

[0045] S1. Select the reaction bottom layer 1, and process the reflection detection hole 6 on the reaction bottom layer 1;

[0046] S2. Paste the reaction layer 2 provided with the reaction area 7 on the reaction bottom layer 1 through double-sided adhesive tape, and ensure that the center of the reaction area 7 is aligned with the reflection detection hole 6;

[0047] S3. Prepare the triglyceride detection reagent according to the ratio, and drop the prepared triglyceride detection reagent to the reaction zone 7 of the reaction layer 2 with a liquid dispenser. Drying, the drying temperature is 37°C, and the drying time is 20min;

[0048] S4. Paste the blood filter layer 3 on the reaction layer 2 to ensure that the blood filter area 8 on the blood filter layer 3 is aligned with the reaction area 7;

[0049]S5. Paste the capill...

specific Embodiment 1

[0053] A triglyceride detection reagent includes 0.5KU / ml lipoprotein lipase, 0.3KU / ml diaphorase, 0.32KU / ml glycerol dehydrogenase, 5mmol / L coenzyme I and 2mmol / L chlorine The nitro blue tetrazolium also contains a mass fraction of 0.2% TritonX-100 solution, 0.05mol / L Tris-HCl buffer solution, and the pH of the Tris-HCl buffer solution is 8.9.

specific Embodiment 2

[0055] A triglyceride detection reagent includes 1.0KU / ml lipoprotein lipase, 0.4KU / ml diaphorase, 0.64KU / ml glycerol dehydrogenase, 10mmol / L coenzyme I and 4mmol / L chlorine The nitro blue tetrazolium also contains a mass fraction of 0.2% TritonX-100 solution, 0.05mol / L Tris-HCl buffer solution, and the pH of the Tris-HCl buffer solution is 8.9.

technical field

[0001] The invention relates to the technical field of triglyceride detection, in particular to a triglyceride detection reagent, a detection test paper and a preparation method for the test paper. Background technique

[0002] The currently widely used dry chemical test method for triglycerides is to use lipoprotein esterase, glycerol kinase, glycerol phosphate oxidase coupled Trinder reaction. However, the redox chromogen in this system is very unstable when exposed to air or light, and if it is not stored properly, it is easy to oxidize in advance and change color before the product is used, resulting in product failure or inaccurate test results.

[0003] Existing solutions such as patent CN105295441B add various additives such as color developer stabilizer, reducing agent, chelating agent, auxiliary stabilizer, etc. to the formula, so that the color developer can maintain better color rendering ability; patent CN102128919B also uses Two kinds of color s...

Examples

preparation example Construction

[0044] The above-mentioned preparation method of the detection test paper added with the detection reagent of triglycerides, the specific steps are:

[0045] S1. Select the reaction bottom layer 1, and process the reflection detection hole 6 on the reaction bottom layer 1;

[0046] S2. Paste the reaction layer 2 provided with the reaction area 7 on the reaction bottom layer 1 through double-sided adhesive tape, and ensure that the center of the reaction area 7 is aligned with the reflection detection hole 6;

[0047] S3. Prepare the triglyceride detection reagent according to the ratio, and drop the prepared triglyceride detection reagent to the reaction zone 7 of the reaction layer 2 with a liquid dispenser. Drying, the drying temperature is 37°C, and the drying time is 20min;

[0048] S4. Paste the blood filter layer 3 on the reaction layer 2 to ensure that the blood filter area 8 on the blood filter layer 3 is aligned with the reaction area 7;

[0049]S5. Paste the capill...

specific Embodiment 1

[0053] A triglyceride detection reagent includes 0.5KU / ml lipoprotein lipase, 0.3KU / ml diaphorase, 0.32KU / ml glycerol dehydrogenase, 5mmol / L coenzyme I and 2mmol / L chlorine The nitro blue tetrazolium also contains a mass fraction of 0.2% TritonX-100 solution, 0.05mol / L Tris-HCl buffer solution, and the pH of the Tris-HCl buffer solution is 8.9.

specific Embodiment 2

[0055] A triglyceride detection reagent includes 1.0KU / ml lipoprotein lipase, 0.4KU / ml diaphorase, 0.64KU / ml glycerol dehydrogenase, 10mmol / L coenzyme I and 4mmol / L chlorine The nitro blue tetrazolium also contains a mass fraction of 0.2% TritonX-100 solution, 0.05mol / L Tris-HCl buffer solution, and the pH of the Tris-HCl buffer solution is 8.9.

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